The red blood cell proteome and interactome identify a Band 3-BLVRB axis regulating hypoxic metabolic adaptation

Key Points. Deep proteomics defines a complete, contamination-free RBC proteome (3,775 proteins) and O2-dependent interactome by Xlinking proteomicsThe Ban

ashpublications.org

Photosensitizer proximity labeling captures the lipid and protein interactomes | Nature Chemical Biology

The physical properties of cellular membranes are influenced by protein and lipid interactions. In situ proximity labeling interactomic methods are well suited to characterize these dynamic and often fleeting interactions. Yet, available methods require distinct chemistries for proteins and lipids. Here we establish a singlet oxygen-based photocatalytic proximity labeling platform (POCA) that reports intracellular interactomes for both proteins and lipids using cell-penetrant photosensitizer reagents. Cholesterol-directed POCA captured known and unprecedented cholesterol-binding proteins, including protein complexes sensitive to intracellular cholesterol levels and proteins uniquely captured by physiologically relevant lipoprotein uptake. Protein-directed POCA accurately mapped intracellular membrane complexes, defined sterol-dependent changes to the interactome of the cholesterol transport protein Aster-B and revealed singlet oxygen-mediated domain-specific Aster crosslinking. More br

doi.org

Red Blood Cells Soak Up Sugar at High Altitude, Protecting Against Diabetes

New study shows red blood cells act as hidden glucose sponges in low-oxygen conditions, explaining why people living at high altitude have lower diabetes rates and pointing toward new treatments.

bit.ly

Cracking the Code of Blood - Transfusion Today - October 2025

isbtweb.foleon.com

Glyoxalase 1 is a proadipogenic gene

Diabetes is one of the most prevalent and widespread diseases, with the majority of cases stemming from prolonged obesity. Obesity occurs through the expansion of adipose tissue in an unhealthy and dy...

jbc.org

The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cells - The EMBO Journal

Oxidative stress is a major driver of cardiovascular disease; however, the fast changes in cellular metabolism caused by short-lived reactive oxygen species (ROS) remain ill-defined. Here, we characte...

link.springer.com

Age-related decline of chaperone-mediated autophagy in skeletal muscle leads to progressive myopathy

Nature Metabolism, Published online: 03 December 2025; doi:10.1038/s42255-025-01412-9Chaperone-mediated autophagy declines with age in skeletal muscle of humans and mice, leading to muscle dysfunction characterized by impaired calcium homoeostasis and mitochondrial function.

bit.ly

Deep Red Blood Cell Proteome Defines the Band 3 N-Terminus Interactome as a Regulator of Hypoxic Adaptation via BLVRB-Dependent S-Nitroso Transfer

Red blood cells (RBCs) have long been regarded as passive oxygen carriers, yet growing evidence reveals a complex, dynamic proteome independent of de novo gene expression. Here, we define the erythroc...

doi.org

Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia

Reduced levels of the essential metabolite heme are a common feature of acute myeloid leukemia, and consequently, leukemic cells are highly sensitive to inhibition of de novo heme synthesis. Blockade ...

cell.com

Genetic architecture of the murine red blood cell proteome reveals central role of hemoglobin beta cysteine 93 in maintaining redox balance

Keele et al. uncover the genetic architecture of the proteome of fresh and stored red blood cells using a multi-omics analysis of 350 genetically diverse mice. Their work reveals how a hemoglobin redo...

cell.com

Tumour sampling conditions perturb the metabolic landscape of clear cell renal cell carcinoma - Nature Communications

Yong et al. highlight how sampling conditions affect metabolic profile in renal cancer, showing that prolonged ischemic exposure disrupts tissue metabolome stability and masks important phenotypes, su...

nature.com

Cover - Transfusion Today - October 2025

isbtweb.foleon.com

A cGAS-mediated mechanism in naked mole-rats potentiates DNA repair and delays aging

Efficient DNA repair might make possible the longevity of naked mole-rats. However, whether they have distinctive mechanisms to optimize functions of DNA repair suppressors is unclear. We find that na...

science.org

Reprogramming neuroblastoma by diet-enhanced polyamine depletion - Nature

Enhanced polyamine depletion in neuroblastoma models decreases translation of mRNA codons with adenosine in the third position, reprogramming the tumour proteome away from cell cycle progression and towards differentiation.

nature.com

Tissue transglutaminase drives fibrin β-chain cross-linking: a novel fibrin modification observed in trauma patients

Key Points. Trauma patient plasma clots feature entirely novel fibrin β-chain crosslinking not evident in healthy controlsFibrin β-chain crosslinks evident

ashpublications.org

BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states - Nature Metabolism

Rizzollo et al. show that BDH2 participates in iron distribution between cellular compartments, which sets the threshold for the ferroptosis vulnerability of the melanoma cell phenotypes, ultimately a...

doi.org

Study Reveals Caffeine May Undermine Blood Transfusion Effectiveness

Study Reveals Caffeine May Undermine Blood Transfusion Effectiveness

news.cuanschutz.edu

Evidence that mitochondria in macrophages are destroyed by microautophagy - Nature Communications

Mitophagy, the selective degradation of mitochondria, has been thought to occur via macroautophagy. Here, the authors present data indicating that microautophagy also mediates mitophagy in macrophages...

nature.com

GPX4 regulates lipid peroxidation and ferroptosis of stored red blood cells

Red blood cell (RBC) membrane lipid peroxidation during blood bank storage profoundly impacts transfusion efficacy; however, the genetic determinants …

sciencedirect.com

From Articles to Code: On-Demand Generation of Core Algorithms from Scientific Publications

Maintaining software packages imposes significant costs due to dependency management, bug fixes, and versioning. We show that rich method descriptions in scientific publications can serve as standalon...

arxiv.org