Emmanuele Severi
@emmseveri.bsky.social
Research Fellow at Northumbria University: phage, synthetic biology, sialic acid, transport proteins. He/him.
#phagesky #microsky #phage www.biorxiv.org/content/10.6...
Sequential assembly in vivo of the bacteriophage P22 genome translocation channel
Bacteriophage P22 ejects four internal proteins into infected cells to assemble a channel for genome translocation across the cell envelope. However, how these proteins cooperate to build the trans-en...
biorxiv.org
#microsky #phagesky T7’s phospho-bombing of host proteins overcomes (-whelms) E. coli #phage defence mechanisms www.nature.com/articles/s41...
Pervasive phosphorylation by phage T7 kinase disarms bacterial defences - Nature
The T7 bacteriophage deactivates bacterial defence systems through T7K phosphorylation of nearly all host and phage proteins during infection.
nature.com
My PhD work with my awesome mentor Chris Waters and collaborator Jeff Barrick looking at how phages encode contingency loci to overcome phage defense systems and potentially other stresses via phase variation is out! www.nature.com/articles/s41...
Phage-encoded contingency loci enable bet-hedging against host defence mechanisms - Nature Microbiology
Diverse E. coli phage genomes have hypermutable DNA regions that promote reversible frameshift mutations through DNA polymerase slippage, generating population heterogeneity that enables host exploita...
nature.com
#microsky #phagesky #phage Phase variation FTW www.nature.com/articles/s41...
Phage-encoded contingency loci enable bet-hedging against host defence mechanisms - Nature Microbiology
Diverse E. coli phage genomes have hypermutable DNA regions that promote reversible frameshift mutations through DNA polymerase slippage, generating population heterogeneity that enables host exploita...
nature.com
#microsky #phagesky #phage journals.plos.org/plospathogen...
Intact architectures of myophage phi92 in extended and contracted states
Author summary With the increasing severity of the problem of drug-resistant bacteria, phage therapy is regaining renewed attention as an effective alternative strategy. Myophage phi92 exhibits broad-...
journals.plos.org
#microsky #phagesky #phage defence www.nature.com/articles/s41...
Antiviral defence is a conserved function of diverse bacterial DNA glycosylases - Nature Microbiology
DNA glycosylases, typically used for DNA repair, can also protect bacteria against phages that would otherwise evade host immunity by incorporating modified bases into their genomes.
nature.com
#microsky #phagesky #phage www.science.org/doi/10.1126/...
Generative design of bacteriophages with genome language models
Many important biological functions arise not from single genes but from complex interactions encoded by entire genomes. We report the first generative design of complete bacteriophage genomes using g...
science.org
A novel stress response pathway mediates biofilm architecture in Pseudomonas aeruginosa PLOS Pathogens by Ainelen Piazza et al from Jakob Malone @johninnescentre.bsky.social with @friendlymicrobe.bsky.social journals.plos.org/plospathogen...
A novel stress response pathway mediates biofilm architecture in Pseudomonas aeruginosa
Author summary Biofilm formation by the opportunistic pathogen Pseudomonas aeruginosa represents a major clinical challenge, with biofilms typically associated with chronic infection, increased antibi...
journals.plos.org
#glycotime #glycotimesky Finally, the structure of the #sialic acid-binding SBP from the Streptococcal ABC transporter is determined! And binding alpha-NeuAc no less - interesting! Only one SBP left to solve now… www.nature.com/articles/s42...
nature.com
Hello Bluesky! 👋 We're a research group at Imperial College working at the interface of chemistry, biology & biotechnology. 🔬 #BiosyntheticPathways, #NaturalProducts & #Antibiotics 🧬 #EnzymeStructure, #EnzymeMechanism & #EnzymeEngineering ⚗️ #Biocatalysis & #SustainableSynthesis. News to follow
#glycotime #glycotimesky From Alan Cartmell and collaborators: sulfatases from Akk muciniphila involved in mucin degradation www.nature.com/articles/s41...
Unique adaptations in sulfatases underpin colonic mucin degradation by Akkermansia muciniphila - Nature Microbiology
Structural and biochemical follow-up of a serendipitous discovery reveals that Akkermansia muciniphila carbohydrate sulfatases are uniquely adapted to digest colonic mucins in collaboration with other...
nature.com
#microsky Leu auxotrophy as genetic marker for Mycobacterium abscessus #synbio journals.asm.org/doi/10.1128/...
Leucine auxotrophic marker for genetic manipulation of Mycobacterium abscessus | Applied and Environmental Microbiology
The development of a leucine auxotroph-based genetic system for Mycobacterium abscessus addresses critical challenges in mycobacterial genetics. By avoiding established antibiotic resistance markers, ...
journals.asm.org
Looking for a 𝐩𝐨𝐬𝐭𝐝𝐨𝐜 in phage research? A 𝟐𝟒-𝐦𝐨𝐧𝐭𝐡 position is available at 𝐂𝐘 𝐂𝐞𝐫𝐠𝐲 𝐏𝐚𝐫𝐢𝐬 𝐔𝐧𝐢𝐯𝐞𝐫𝐬𝐢𝐭é starting 𝐉𝐚𝐧𝐮𝐚𝐫𝐲 𝟐𝟎𝟐𝟕 to develop genetically engineered therapeutic phages against 𝘚. 𝘢𝘶𝘳𝘦𝘶𝘴 biofilms. 📄Details in the attached announcement—please share! #PhageResearch #Postdoc #ISVM
#microsky #phagesky Lanthipeptides involved in #phage defence! What a blast!! www.cell.com/cell-host-mi...
A family of lanthipeptides with anti-phage function
Shomar, Guillaume, et al. report the genomics-driven discovery of a family of more than 2,000 lanthipeptide biosynthetic gene clusters with anti-phage potential. They experimentally demonstrate anti-p...
cell.com
#microsky #phagesky #phage The secret life of prophages journals.asm.org/doi/10.1128/...
From hijackers to architects: the role of prophages in bacterial genome evolution | Journal of Bacteriology
Bacteriophages (phages) are one of the most important drivers of bacterial evolution, regulating physiology, genetic diversity, population dynamics, and coevolution through complex host-phage interact...
journals.asm.org
#phagesky #microsky #phage www.cell.com/cell-reports...
A receptor-centered approach identifies Lom as a LamB-bound superinfection exclusion factor in bacteriophage λ
Ge et al. develop a receptor-centered strategy to identify phage proteins that target host receptors during infection and discover the λ outer-membrane protein Lom. Lom binds and occludes LamB, blocki...
cell.com
New #glycotime #glycotimesky paper reporting the super-fun isolation of “rule-breaking” #sialic acid transporter mutants! The phenotype is a real tease, much to ponder about. Worth a read: febs.onlinelibrary.wiley.com/doi/10.1002/...
FEBS Press
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and sub...
febs.onlinelibrary.wiley.com
High-quality phage assembly from metagenomes with PALACE www.nature.com/articles/s41...
High-quality phage assembly from metagenomes with PALACE - Nature Biotechnology
PALACE combines homology features and deep learning to improve phage assembly from metagenomics.
nature.com
Registration is now open for Synthetic Biology UK 2026. Join the UK synthetic and engineering biology community on 2–3 November in Hampshire to explore the future of biological design and innovation. https://ow.ly/x7KT50ZlVF9 🧪
What are your favorite papers that linked patients, traced outbreaks, or distinguished epidemiological hypotheses on the careful basis of just a few SNVs? Like ~ <20? Bacterial best, viruses OK too
@breakliquid.bsky.social and I are delighted to say our OpenEVO preprint is finally live! An open source hand-made turbidostat that's modular, affordable, and made to be customized. Designed with love for the research community. Enjoy!!!💚 www.biorxiv.org/content/10.6...
OpenEvo: An Open-Source Platform for Automated Evolution and Analysis
Here we introduce OpenEvo, a fully open-source, low-cost turbidostat platform for automated continuous culture and directed evolution experiments. Existing tools are expensive, complex, or lack open-s...
biorxiv.org
With Eugene Koonin, we wrote a rather comprehensive review on the origin, evolution and organization of the #virosphere. We describe all 10 viral realms and the logic behind them, and so much more. Check it out! comptes-rendus.academie-sciences.fr/biologies/ar...
#microsky New preprint from the lab! Widespread immune systems protect bacteria against conjugative plasmids www.biorxiv.org/content/10.6... Kudos to the extremely talented lead author Ludovic Poiré! Key contributions by @francoisrousset.bsky.social and also help from C. Lesterlin/TacC lab. 🧵⬇️
Widespread immune systems protect bacteria against conjugative plasmids
Conjugative plasmids are a class of mobile genetic elements capable of efficient transfer between bacterial cells. Although they can introduce beneficial traits such as antibiotic resistance to recipi...
biorxiv.org
Are you a UK-based researcher working in microbiology or environmental biotechnology? Researchers at the University of Edinburgh are seeking your views on the natural environment, microbes and microbiomes. Complete their survey by 17 July: https://microb.io/4y7OWoX
#microsky #phagesky #phage 440K tail-fibre mutants… 440K… Mind-boggling doesn’t even start describing this
Vivek Mutalik@vivekmutalik.bsky.social · 3mo agoNew preprint!! 🎉 A cool collaborative work with @copabio.bsky.social and Chase Beisel teams on the phage editing technology. @sarshad.bsky.social led the work from our side to validate & characterize the lib. #phage #phagesky www.biorxiv.org/content/10.6... Work supported by: phagefoundry.org
New preprint!! 🎉 A cool collaborative work with @copabio.bsky.social and Chase Beisel teams on the phage editing technology. @sarshad.bsky.social led the work from our side to validate & characterize the lib. #phage #phagesky www.biorxiv.org/content/10.6... Work supported by: phagefoundry.org
Targeted DNA nicking enables efficient, single-step and counterselection-free editing of bacteriophage genomes
Genetic manipulation of bacteriophages is essential for interrogating phage biology and advancing antimicrobial therapies. However, current genome editing approaches can be inefficient, require multip...
biorxiv.org
#microsky #phagesky #phage www.nature.com/articles/s41...
END nucleases are antiphage defence systems targeting multiple phages with modified genomes - Nature Microbiology
The END nuclease targets eight phage families with at least ten different known DNA modifications, but not unmodified DNA. Its specificity is dictated by the C-terminal iEndoIII domain. To combat END,...
nature.com
Access Microbiology is recruiting Editor Mentees across all fields of microbiology! Applications close 31 July 2026. For more information and details on how to apply, visit our website: https://microb.io/Vacancies #AcMi
Recruiting a postdoc to join my lab at @lshtm.bsky.social to work in an MRC porgramme grant funded project to dissect the gut colonziation by Klebsiella. In vivo and in vitro models, and new approaches to identify Klebsiella factors involved. jobs.lshtm.ac.uk/vacancy.aspx...
Job Opportunity at LSHTM: Research Fellow
The London School of Hygiene & Tropical Medicine (LSHTM) is one of the world’s leading public health universities. Our mission is to improve health and health equity in the UK and worldwide; working i...
jobs.lshtm.ac.uk
Time from submission to acceptance: 3 UK Prime Ministers.