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Natalie Turner

@natalieturnerau.bsky.social

Postdoc @ Scripps Research - Yates lab | Extracellular Vesicles | Mass Spectrometry | Proteomics | Bioinformatics. Multi-omics enthusiast. Here to talk science 👩🏻‍🔬. 🇦🇺 🇺🇸 🇬🇧 .

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Natalie Turner@natalieturnerau.bsky.social · 3mo ago

Hundreds of histone PTMs can be identified in under 2 h using our RIPUP dual protease workflow: Full paper out now in Analytical Chemistry! @pubs.acs.org pubs.acs.org/doi/10.1021/...

Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass Tags

Histone post-translational modifications (PTMs) alter chromatin dynamics and contribute to the regulation of gene expression in health and disease, yet mass spectrometry-based histone PTM analysis rem...

pubs.acs.org

Natalie Turner@natalieturnerau.bsky.social · 5mo ago

We have an update to our histone RIPUP preprint- now featuring a quantitative analysis to demonstrate the robustness of the method for detecting changes in PTM abundance! Link 👇 www.biorxiv.org/content/10.6...

Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass Tags

Histone post-translational modifications (PTMs) alter chromatin dynamics and contribute to the regulation of gene expression in health and disease, yet mass spectrometry-based histone PTM analysis remains constrained by inefficient sample preparation workflows. Here, we develop RIPUP (Rapid Identification of histone PTMs in Underivatized Peptides), a streamlined multi-protease workflow that reduces sample preparation to hours while improving PTM coverage and quantitative accuracy. Systematic evaluation of Arg-C Ultra and a recombinant (r)-Chymotrypsin protease under varied conditions, including standard derivatization with propionic anhydride and tandem mass tag (TMT) labeling, demonstrated that Arg-C Ultra with TMT labeling achieves a detection of total PTM that exceeds Trypsin-based approaches. Using the HiP-Frag computational framework for unrestrictive PTM identification, we discovered that TMT's tertiary amine provides charge compensation that rescues the ionization of negatively charged acylations revealing 58 succinylation and 31 glutarylation sites - a 'dark epigenome' largely undetected by propionylation-based methods. Complementary digestion with Arg-C Ultra and r-Chymotrypsin provides orthogonal sequence coverage, enabling detection of PTMs in H2A variants, linker histones, and regions poorly represented by arginine-specific cleavage alone. In HEK293T cells treated with the pan-sirtuin inhibitor nicotinamide, RIPUP quantified 112 statistically significant peptidoforms (adj p < 0.05), predominantly increasing with NAM dose (88 up, 24 down). Application of RIPUP to frozen-thawed rat hippocampal sections within a 3-hour workflow identified >200 PTMs including H3 K27/K36/K37 methylation, H4 N-terminal acetylation patterns, and H2A K118/K119 ubiquitination. This rapid, high-efficiency platform enables timely discovery of epigenetic mechanisms and accelerates the path from PTM identification to therapeutic target validation. ### Competing Interest Statement The authors have declared no competing interest. National Institute on Alcohol Abuse and Alcoholism, T32 AA007456, AA013498, P60 AA006420, AA017447, AA029841, AA021491 Paul and Cleo Schimmel Endowed Chair

biorxiv.org

Natalie Turner@natalieturnerau.bsky.social · 8mo ago

New in Nature Comms: Autophagic EVs (AEVs) are a distinct vesicle subtype, not just #exosomes! Mao et al. used proteomics to identify unique markers (autophagic cargos, ESCRT III components, Rab13) for these <100nm vesicles released via amphisomes. www.nature.com/articles/s41... #Proteomics

Autophagic extracellular vesicles (AEVs) are distinct from exosomes and play crucial roles in viral infections - Nature Communications

The authors identify a subtype of small extracellular vesicles, termed autophagic extracellular vesicles. This type of vesicle exhibits biomarkers different from exosomes and plays a crucial role in v...

nature.com

Nature Protocols@natprot.nature.com · 9mo ago

New Article! Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles

Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles

Nature Protocols, Published online: 09 January 2026; doi:10.1038/s41596-025-01286-xThis protocol covers the acoustics-based separation of biological particles such as viruses and single extracellular vesicles from biofluids, including plasma and saliva, with high purity.

dlvr.it

Natalie Turner@natalieturnerau.bsky.social · 11mo ago

Yes, they used a GNA resin for affinity capture- to demonstrate that small (s)EVs did not carry the mannose+ signature (the positive correlation improved after sEV depletion), thus large EVs were considered the ‘pathogenic’ ones. High variability limits the interpretation in this study.

Jon Douglas@atranscendedman.bsky.social · 11mo ago

At UCSF, researchers studied 45 people and found that those with Long COVID had higher levels of mannosylated extracellular vesicles in their blood Using a GNA resin from snowdrop plants in a device called the Hemopurifier, they were able to remove them www.biorxiv.org/content/10.1...

Natalie Turner@natalieturnerau.bsky.social · 12mo ago

Very thorough and thought-provoking review on #extracellularvesicles - clinical applications in cancer diagnostics, vaccines, and drug delivery systems, by Greening et al. Published in Nature Reviews Clinical Oncology: www.nature.com/articles/s41...

Clinical relevance of extracellular vesicles in cancer — therapeutic and diagnostic potential - Nature Reviews Clinical Oncology

Extracellular vesicles (EVs), a diverse range of membrane-delimited particles, have multiple cellular functions and, when released by cancer cells, can promote tumour growth and metastatic dissemination. The authors of this Review describe advances in the development of EVs as biomarkers and cancer therapeutics, focusing on clinical translation of EVs into diagnostic and therapeutic clinical tools.

nature.com

Ian Weissman, DO@drianweissman.bsky.social · last yr.

Obesity may directly drive Alzheimer’s disease. Scientists discovered that tiny messengers released by fat tissue, called extracellular vesicles, can carry harmful signals that accelerate the buildup of amyloid-β plaques in the brain. www.sciencedaily.com/releases/202...

Fat may secretly fuel Alzheimer’s, new research finds

New research from Houston Methodist reveals how obesity may directly drive Alzheimer’s disease. Scientists discovered that tiny messengers released by fat tissue, called extracellular vesicles, can ca...

sciencedaily.com

Natalie Turner@natalieturnerau.bsky.social · last yr.

EV-microRNAs may serve as early predictors of subfertility in dairy cows. Congrats Pevindu for this final publication from his PhD and our time with Prof Murray Mitchell at QUT in Brisbane, Australia. 🇦🇺 #extracellularvesicles #biomarkers isevjournals.onlinelibrary.wiley.com/doi/10.1002/...

MicroRNA (miRNA) in Plasma Small Extracellular Vesicles (sEV) as Potential Early Indicators of Dairy Cow Subfertility

Pregnancy and live birth rates are commonly used metrics to define fertility in humans and animals. The impact of aberrant microRNA (miRNA) expression on fertility-related genes represents a signific....

isevjournals.onlinelibrary.wiley.com

Sierra Palumbos@palumbos.bsky.social · last yr.

Very excited to share my postdoc work- out now at PNAS! Learn more about how neurons facing chronic autophagic stress compensate by upregulating two secretion pathways Autophagic stress activates distinct compensatory secretory pathways in neurons | PNAS www.pnas.org/doi/10.1073/...

PNAS

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

pnas.org

Natalie Turner@natalieturnerau.bsky.social · last yr.

💡 This week I attended #ASMS2025 in Baltimore to present 'Deep proteome characterization unveils distinct secretory phenotypes of Wild Type and familial Alzheimer's Disease organoids'. Our findings shed light on how secretory differences may influence disease progression and biomarker discovery.

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Michael Baym@baym.lol · last yr.

Yesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab

A screenshot of the termination notice showing "Outstanding Investigator Grants"A screenshot of the termination notice with "This award is terminated effective the date of this award, due to unsafe antisemitic actions that suggest the institution lacks concern for the safety and wellbeing of Jewish students." highlighted