Matt Doyle
@doylemt1.bsky.social
🇦🇺 Senior Lecturer at @sydneyuni.bsky.social and former NIHer. Research: Bacterial outer membrane biogenesis - membrane protein folding - protein translocation across membranes - novel antibiotics. He/him.
So, @arc-gov-au.bsky.social: Why would the UK want to do this? Could it be that much shorter grant processing times are … a good thing? Could there be a competitive disadvantage in taking 4.8 times longer to process grants? What do you think, Minister @jasonclaremp.bsky.social?
The UK government’s research grant agency wants to cut grant processing times from 195 days to 90. Meanwhile, the Australian equivalent, has increased it from 195 days to at least 435 (DP26 vs. DP27). This is for comparable, “applicant led grants”. Make it make sense.
UKRI pledges to cut grant processing time in half - Research Professional News
Funder’s new five-year strategy aims to boost national priority fields, while backing curiosity-driven research
researchprofessionalnews.com
Read our new paper! We show how the protease BepA binds the BAM complex and undergoes striking conformational changes, embedding its water-soluble lid into the membrane to cleave outer membrane proteins. www.nature.com/articles/s41... #cryoEM #astburycentre
Had an amazing day at the @Sydney_Uni, presenting our work at the SABA BacChats Seminar Series! Great scientific discussions throughout the day. Thanks again for the warm hospitality, I thoroughly enjoyed the visit! @doylemt1.bsky.social and @BilgeErcan, thank you both for everything.
This amazing work by @danialv.bsky.social and Bodan Hu is now out in peer-reviewed form at @cp-cell.bsky.social with a lot of new simulations! Check it out if interested in lipid transport!
Vanni Lab at UNIFR, Switzerland@labvanni.bsky.social · 7mo agoHappy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7) www.biorxiv.org/content/10.6...
Some very cool simulations, look at those little guys climb that ladder! #MolecularNodes
@corrylab.bsky.social · 2mo agoExcited to share this paper from @yiechanglin.bsky.social showing how lipids can move between bacterial membranes along the bridge like protein TamB. Also happy to be able to post the cool movie. 🎥🌟 #MDsimulations #StructuralBiology doi.org/10.1016/j.bp...
why would anyone vote ON, we would end up like the USA: ruled by incompetents, politicians lining their pockets, grifting for mega billionaires, cost of living skyrockets, science and facts ignored to detriment of health and community. If you cant vote labour or lib vote green or independent
Excited to share this paper from @yiechanglin.bsky.social showing how lipids can move between bacterial membranes along the bridge like protein TamB. Also happy to be able to post the cool movie. 🎥🌟 #MDsimulations #StructuralBiology doi.org/10.1016/j.bp...
This has been a long journey and it's exiting to see our story about the Atg2-18 lipid transfer complex, headed by Sabrina Chumpen, published in @embojournal.org! Great teamwork of the labs @arnemoeller.bsky.social, Stefano Vanni, Fulvio Reggiori and Christian Ungermann! #cryoEM #autophagy #membrane
The EMBO Journal@embojournal.org · 3mo agoAtg18 interaction positions Atg2 for efficient lipid transfer into phagophore elongation Sabrina Chumpen Ramirez, @dmitryshvarev.bsky.social et al link.springer.com/article/10.1...
Static vs. cidal: it’s not complex; it’s simply incorrect @bradspellberg.bsky.social et Al Honored to be part of this amazing group who challenges dogmas and debunk ID myths #idsky #microsky journals.asm.org/doi/10.1128/...
What a fascinating story is hiding in bioRxiv!! An elaborate work of @doylemt1.bsky.social an co-workers to understand the function of the bacterial TAM machinery ❤️🔥 and here it is, a rich body of evidence for the "ancient" bridge for the lipid transfer 👇 www.biorxiv.org/content/10.6...
biorxiv.org
Join us on campus on Thursday, 26 March 2026 at 1pm for a seminar by Dr Matthew Doyle @doylemt1.bsky.social, Senior Lecturer from the Faculty of Medicine and Health, The University of Sydney. Details: rb.gy/4uw4uz
And now published as an Observation! Many congrats to @weeboont.bsky.social for discovering that AsmA superfamily proteins, YhdP/TamB/YdbH, localize to the cell poles in E. coli, curiously AFTER division has occurred! 1/3 #MicroSky journals.asm.org/doi/10.1128/...
Polar localization of putative phospholipid transporters in Escherichia coli | mBio
The outer membrane (OM) of Gram-negative bacteria serves as an effective permeability barrier and confers intrinsic antibiotic resistance. This barrier function requires distinct distribution of lipid...
journals.asm.org
Shu-Sin Chng@figlegend.bsky.social · last yr.@weeboont.bsky.social talked about our lab's recent preprint where we report AsmA superfamily proteins, believed to be phospholipid transporters, localizing to cell poles in E. coli. Really interesting observations, with impt implications for OM assembly! Read it 👇! www.biorxiv.org/content/10.1...
My predictions are that grant delays have a much more severe impact on Australia's productivity than any real foreign interference enabled by any ARC grants.... maybe the delay was always the intended interference.
Danny Kingsley@dannykay68.bsky.social · 6mo agoDELAY: the work associated with protecting Australian research from foreign interference are causing hold ups and issues for grant applications. www.timeshighereducation.com/news/grant-d... “The requirements we now have to go through…are more extensive and time-consuming than we had imagined,”
DELAY: the work associated with protecting Australian research from foreign interference are causing hold ups and issues for grant applications. www.timeshighereducation.com/news/grant-d... “The requirements we now have to go through…are more extensive and time-consuming than we had imagined,”
Grant delays feared as ARC ramps up ‘due diligence’
Researchers brace for renewed funding uncertainty as security checks prove ‘more time-consuming than imagined’
timeshighereducation.com
A. baumannii is one of the most notorious multidrug-resistant pathogens, capable of forming drug-tolerant biofilms that make infections exceptionally difficult to treat. But how are these complex microbial fortresses built? Just published in Nat Comms. pubmed.ncbi.nlm.nih.gov/41654547/
Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly - PubMed
Many Gram-negative nosocomial pathogens rely on adhesive filaments, known as archaic chaperone-usher pili, to establish stress- and drug-resistant, multi-layered biofilms. Here, we uncover the mechanism by which these pili build three-dimensional (3D) biofilm architectures. In situ analyses of Acine …
pubmed.ncbi.nlm.nih.gov
Beautiful work Deb! Check out these structures? Biofilms are very interesting. 🧶🧬
Debnath Ghosal@debnathghosal.bsky.social · 6mo agoA. baumannii is one of the most notorious multidrug-resistant pathogens, capable of forming drug-tolerant biofilms that make infections exceptionally difficult to treat. But how are these complex microbial fortresses built? Just published in Nat Comms. pubmed.ncbi.nlm.nih.gov/41654547/
Fabien Munder - New strategies to fight AMR. Bacterial competition via L-type pyocins; targeting (1) BamA EC loop 6, (2) beta-strand 1. Inhibit beta barrel assembly by blocking the BAM barrel lumen. Increase cell permeability - holes in the OM. 👏 (EMReady post-processing) 🤓 #lorneproteins2026
Really beautiful cryoET of periplasmic flagella structures and amazing animations
Lorne Proteins@lorneproteins.bsky.social · 6mo agoS3: Luca Troman-Spirochete flagellar motors rotate in opposite directions; periplasmic flagella are more complex. Treponema denticola. 2 motors, thick and thin flagella. SPA Native flagella isolation w optiprep cushion, inner core, outer sheath, mystery density - modelangelo! 🕵♀️ #lorneproteins2026
Neg charged lactate binds pocket when His positively charged. Lactate binds favourably, but only lactic acid can leave. Proton enters to charge His, attracts negative lactate, proton neutralises lactate to lactic acid, then LA leaves.
First speaker Ashleigh Kropp of the most fun session of the Lorne Protein Meeting the Anders Young Investigators session. @lorneproteins.bsky.social
Yan Jiang is the next Anders session speaker presenting her work on the SLC1A transporter. @lorneproteins.bsky.social
Emily Furlong (UQ alumnus 😄) talking about her new work on ABC transporters. @emfurlong.bsky.social @lorneproteins.bsky.social
Session 7 ⚡️🌩 talks time!! Jack Zeng Unravelling Promiscuity - OCT1 gating - structures of ligand bound states. Interrogating binding and transport specificity and mechanism. #lorneproteins2026
No, I was wrong. The ARC just posted on X – you know, the platform that whose offices have been raided by French police because it produces and hosts child exploitation materials? Yes, that X.
Just noting that ARC is still on X/Twitter. Despite it now being clear that that platform produces child exploitation materials, they remain active there. If you interact with ARC staff, I really think they need to answer this question. It's moved being simply "disconnected from researchers" to … 🤢
The coolest finding of my PhD is finally out! E. coli has a 7th RND pump that is present in phylogroups B2/D/E/F but absent in A/B1/C. As a result EefABC has been absent in all K-12 RND studies! Co-authored by the very talented Dr. Lizzy Darby. @jessicamablair.bsky.social doi.org/10.1099/mgen...
The Resistance-Nodulation-Division efflux pump EefABC is highly conserved within lineages of E. coli commonly associated with infection
Resistance-nodulation-division (RND) efflux pumps confer multidrug resistance in Gram-negative bacteria and are critical for many physiological functions including virulence and biofilm formation. The...
doi.org
💊 We are excited to share our preprint that describes an inhibitor of the widespread and highly conserved Two-Partner Secretion (TPS) system that is critical for Gram-negative pathogens to export a multitude of diverse virulence factors. (1/6) www.biorxiv.org/content/10.6...
First inhibitor of a bacterial two-partner secretion system.
Two-partner secretion system transporter proteins (TpsB) are widely conserved across Gram-negative pathogens. TpsB family proteins secrete exoprotein virulence factors that perform a myriad of functions such as adhesion and immune modulation. Despite their incredible importance in bacterial infectious disease, TpsB inhibitors have not yet been discovered. Here, we describe a potent inhibitor of FhaC, a TpsB protein produced by Bordetella spp . FhaC secretes the exoprotein FhaB that is essential for the establishment of whooping cough. We designed a peptide called P1 that we predicted would prevent substrate binding and lock FhaC in a secretion-inactive state. Simulations and biochemical assays supported our hypothesis and identified interactions important for P1 binding to FhaC. Strikingly, we observed that the peptide strongly inhibited FhaB secretion from clinical isolates and broadly reduced correlates of virulence. Together, this work provides a strong case for further development of a novel class of anti-TpsB anti-virulence compounds. ### Competing Interest Statement The authors have declared no competing interest. National Institute of Allergy and Infectious Diseases, R21AI180112
biorxiv.org
Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7) www.biorxiv.org/content/10.6...
