Halim Loukil

@loukillab.bsky.social

Principal Investigator | Sanford Research Institute & Assistant Professor, Pediatrics | University of South Dakota #cilia #rarediseases #centrioles #centrosome #brain #neuroscience #cilium #cilia http://Loukil-Lab.com

If you work on #PrimaryCilia 🔬 & spend too much time on manual image analysis, this might help! We built Cilia SubQ — automated cilia detection with analytical pipelines for basal body, transition zone, ciliary tip, and more (~8x faster ⏱️). Free on OSF 🦾 Congrats Ellie Menzel! 🎉 osf.io/hm38f

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🥳 Our new paper on C2CD3 is out in @plosbiology.org With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET. Big congrats to @ebertiaux.bsky.social and all involved.

PLOS Biology@plosbiology.org · 10mo ago

How does C2CD3 contribute to distal appendage formation in centrioles? @centriolelab.bsky.social show that C2CD3 acts as a central architectural organizer of the distal #centriole, bridging the luminal distal ring complex & peripheral appendage sites @plosbiology.org 🧪 plos.io/496XZuH

C2CD3 localizes to a ring structure observed in the lumen of the distal centriole by in situ cryo-electron tomography. Top: Confocal image of an expanded mouse photoreceptor cell immunolabeled for tubulin (magenta) and C2CD3 (green). DC, daughter centriole; BB, basal body; TZ, transition zone; CC, connecting cilium. Scale bar: 500 nm. Middle: In situ cryo-tomogram slices taken along the longitudinal axis of the centriole and connecting cilium. White arrowheads indicate microtubule triplets, black arrowheads indicate microtubule doublets, orange arrowheads mark the luminal ring structure, and yellow highlights the membrane. Scale bar: 50 nm. Bottom:  Model of the architecture of the distal region of the human centriole.

Mentoring is the most important thing most of us can do. Here is a 🧵about what I've learned as a PI running an academic research lab. I hope some of the advice is helpful to those who are just starting their labs and to folks hitting the academic job market in search of an independent position 🧪 1/n