#PERTomics, the systematic functional perturbation of genes and quantification of subcellular, cellular, and organismal responses. To functionally annotate uncharacterized genes, build genetic interaction maps, identify drug dependencies and synergies, and understand polygenetic biology.

DNA guides instead of RNA?! A CRISPR-Cas12a system flips the script, using DNA guides to target RNA. 🔬 Enables direct RNA detection + knockdown 🧬 Reveals a distinct activation mechanism ⚙️ Expands the toolbox for programmable RNA control Big step for CRISPR versatility. www.nature.com/articles/s41...

DNA-guided CRISPR–Cas12a effectors for programmable RNA recognition and cleavage - Nature Biotechnology

Synthetic DNA guides (crDNA) reprogram Cas12a nucleases for RNA targeting.

nature.com

Excited host @hohmannulrich.bsky.social from @imbmainz.bsky.social, along with @manuelkaulich.bsky.social Date: 07.04.2026 Time: 15:00 Venue: Haus 22 Seminar Room 1. (S22-1) Title: ‘Mechanistic insights into nuclear mRNA export and degradation’ @goetheuni.bsky.social @cpi-exstra.bsky.social

A fantastic line up of speakers. Hope to see many of you in wonderful Mainhatten (Frankfurt).

Frankfurt Cancer Institute@loewe-fci.bsky.social · 6mo ago

⏰ Reminder: Abstracts for the Frankfurt Cancer Conference 2026 are due Mar 26! Share your research and connect with leading cancer researchers. 📅 September 2–4, 2026 📍 @Goethe University Frankfurt (Campus Westend) 🔗 www.frankfurtcancerconference.org #FrankfurtCancerConference

Happy to share another paper from the lab. While KEAP1 is often mutated in aggressive lung cancer, its loss can paradoxically suppress tumor growth in certain contexts by inducing Nrf2-driven reductive stress. link.springer.com/article/10.1...

Targeting ubiquitin signaling vulnerabilities in KEAP1-inactivated lung cancer - The EMBO Journal

Lung cancer cells rely on protein homeostasis regulators, particularly the ubiquitin-proteasome system (UPS), to sustain malignancy. Genetic alterations in UPS components, such as E3 ubiquitin ligases...

link.springer.com

How do immune disease-relevant variants rewire gene regulation in CD4+ T cells? In a collaboration led by Daniel Schraivogel and Lars Steinmetz at @embl.org, we combined two large-scale CRISPRi screens (4.1M cells) to map the downstream cascades of thousands of SNPs.

Out now on bioRxiv! 🧬🖥️ Dewi Moonen, @anniquec.bsky.social and a team led by Lars Steinmetz and Daniel Schraivogel at EMBL have mapped the downstream regulatory networks of thousands of immune disease-relevant variants in CD4+ T cells www.biorxiv.org/content/10.6...

Very proud to see this work out on bioRxiv. Using genome-scale TAP/Perturb-seq we mapped the function of immune-disease variants to target genes and regulatory cascades. Have a look at the 🧵from @anniquec.bsky.social for more info!

Annique Claringbould@anniquec.bsky.social · 6mo ago

🧬 How do immune disease-relevant variants affect gene regulatory networks in CD4+ T cells? 🧪🖥️ We coupled two large-scale CRISPRi screens (>4M cells) to map the downstream cascades of thousands of SNPs More details in the thread below 👇 or in the article 📖 on biorxiv tinyurl.com/CD4screens

So great to this preprint out. It was/is such a pleasure to work with this fantastic team. Congratulations to all of you!

Annique Claringbould@anniquec.bsky.social · 6mo ago

🧬 How do immune disease-relevant variants affect gene regulatory networks in CD4+ T cells? 🧪🖥️ We coupled two large-scale CRISPRi screens (>4M cells) to map the downstream cascades of thousands of SNPs More details in the thread below 👇 or in the article 📖 on biorxiv tinyurl.com/CD4screens

Our preprint is published in MSB! This time with the correct preprint post below! link.springer.com/article/10.1...

Integrative multi-omics defines melanoma drug response networks and ARID1A-dependent resistance mechanisms - Molecular Systems Biology

Resistance to BRAF/MAPK inhibitors is a significant challenge in melanoma treatment, driven by adaptive and acquired mechanisms allowing tumor cells to evade therapy. We explored early signaling respo...

link.springer.com

Evangelia Petsalaki@epetsalaki.bsky.social · 2y ago

We are proud to present "ARID1A-induced transcriptional reprogramming rewires signalling responses to drug treatment in melanoma" led by Charlie Barker and Sumana Sharma in collaboration with EPIC-XS Utrecht, ProtAvio, @sangerinstitute.bsky.social and UofAthens www.biorxiv.org/content/10.1...

The public event "CRISPR Gene scissors in Dresden" is taking place March 26 to 29. The "CRISPR-Roadshow" is teaming up with partners @mpi-cbg.de, @tudresden.bsky.social, and @crtd-tud.bsky.social for a wide range of activities on the CRISPR/Cas method. www.crispr-whisper.de/2025/10/01/d...

Die Genschere in Dresden, 26.3.2026 bis 29.3.2026 - CRISPR-Whisper

Die „CRISPR-Roadshow“ ist ein Projekt des Forschungskonsortiums SPP2141, mit dem Wissenschaft für jedermann verständlich und erlebbar wird. Der SPP2141 ist eine Gruppe von 20 Forschungslaboren, die vo...

crispr-whisper.de

Insights from the 2nd EuroDepMap Symposium are now out in FEBS Letters. Last November at Human Technopole researchers in cancer functional genomics and computational biology discussed challenges and future directions in mapping cancer vulnerabilities and building predictive frameworks. Link ⬇️

Our thoughts, impressions, and presentations of the "2nd EuroDepMap symposium" in Milan (Nov 2025) are online at @febsletters.bsky.social. febs.onlinelibrary.wiley.com/doi/10.1002/...

FEBS Press

Held on 20 November 2025 at Human Technopole in Milan, the 2nd European Cancer Dependency Map Symposium convened experimental and computational scientists to discuss how cancer dependency mapping in ...

febs.onlinelibrary.wiley.com

💫 Thank you to all participants and speakers who joined us at the 2nd @eurocancerdepmap.bsky.social Symposium! Together, we’re advancing #cancer functional genomics across Europe. youtu.be/Qt_ozbzM5js

2nd European Cancer Dependency Map Symposium - 20 Nov 2025

YouTube video by Human Technopole

youtu.be

The Synthetic Lethality and Innovations in CRISPR Screening session begins with @manuelkaulich.bsky.social telling us about exciting CRISPR libraries they have developed and how nuclear mRNA processing can mask the CRISPRko phenotypes @eurocancerdepmap.bsky.social

Our paper benchmarking foundation models for perturbation effect prediction is finally published 🎉🥳🎉 www.nature.com/articles/s41... We show that none of the available* models outperform simple linear baselines. Since the original preprint, we added more methods, metrics, and prettier figures! 🧵

In times when science must defend itself, the decision by the German Research Foundation (DFG) to significantly support German universities through the Excellence Initiative for the next seven years is a signal that stands unparalleled. www.dfg.de/en/news/news...

Announcement of Clusters of Excellence to Receive Funding in the Future, Thursday, 22 May 2025, from approx. 5 pm

dfg.de

Such a wonderful example of what genetic screens can tell us about human patho/physiology. Cudos @mgarnett.bsky.social, Fiona Behan and many more.

Mathew Garnett@mgarnett.bsky.social · last yr.

Six years after the discovery of WRN synthetic lethality in MSI cancers, WRN inhibitors hit center stage again today at #aacr25 with report of the first in human phase 1 trial data for RO7589831. Can’t wait to see it!

Congratulations! & thanks for letting us contribute to this wonderful story.

@thorohde.bsky.social · last yr.

I am excited to announce the publication of our paper in @molsystbiol.org! I am incredibly grateful for this wonderful collaboration with the team of @manuelkaulich.bsky.social. A big thank you to @yasirde.bsky.social, @shawangela.bsky.social and to @maxbillmann.bsky.social! doi.org/10.1038/s443...

A record low…. only 16% of Germans think that Germany can trust the US. A record fast…. never before lost a US president support for its political agenda that fast. www.spiegel.de/politik/deut...

Deutschlandtrend der ARD: Nur 16 Prozent halten USA noch für vertrauenswürdig

Bleiben die USA der wichtigste Verbündete Europas? Die große Mehrheit in Deutschland glaubt einer Umfrage zufolge offenbar nicht mehr an eine gute Zusammenarbeit.

spiegel.de

If a team wished to include combinatorial gene perturbation and bioinformatics in their program, please reach out.

Cancer Research UK@cancerresearchuk.org · 2y ago

Just announced! Seven new Cancer Grand Challenges – with awards of up to £20m – available for research teams around the world to tackle the biggest questions in cancer research. Read more: cruk.ink/4ha3LgZ 1/2

New Gordon Research Conference Alert: Synthetic Lethality Approaches in Oncology. www.grc.org/synthetic-le.... Join us 20-25th July 2025 for an intimate meeting with thought leaders in the field. Great for networking and opportunities for early career scientists to present their research.

80% of Germans (84% participation) voted for democracy. #TogetherAgainstPopulism

Interested in synthetic lethality or how functional genomics can be leveraged for clinical benefit? Then, this is the place to meet: www.grc.org/synthetic-le...

If you search for unexpected phenotypes (genetic interactions), aren't they expected when observed? 🤔

Which rate do you consider appropriate, 10% or 1% ?

Manuel Kaulich@manuelkaulich.bsky.social · 2y ago

Thinking about grant alternatives to fund basic research? Provocative thought - How about getting a royalty rate per accessed publication (per corresponding author)?

Thinking about grant alternatives to fund basic research? Provocative thought - How about getting a royalty rate per accessed publication (per corresponding author)?

📣Registration is now open for an exciting Gordon Conference on synthetic lethality organized by Ben Schwartz (GSK), Mathew Garnett (Sanger), and Francisca Vazquez (DepMap Director, Broad Institute) Highly recommended! Register here grc.org/synthetic-le...

FYI 🔔The abstract deadline for #cshlnetworks is coming up - Jan. 27th! Register to submit your abstract here: bit.ly/40cIfSl @fritzroth.bsky.social @jsvanleeuwen.bsky.social @pedrobeltrao.bsky.social

Network Biology

Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

bit.ly