Ribosomal allostery as a potential regulator of bacterial dormancy 🦠 www.nature.com/articles/s41...

When nature is messaging to finish up our structural study on ribosomes!

My PO said I had to fill out this form for 3 of my papers for the RPPR of my NIGMS MIRA grant in February, or I could not include them in my progress report. It looks like things have gotten even worse since then.

A dividing cell videoed through a microscope. Hot tip: Don't mess around with myosin II paralogs if you don't want your cells to freak out. #CellBiology www.cell.com/cell-reports...

Surprised to discover that Thermo Fisher appears to show a fake western blot for the validation of one of their p53 antibodies. I've added a diagram to show the very similar bands. This does not appear to be one of the "published figures" but their own internal data.

Excited to share our work uncovering SNOR, a ribosome-associated factor that promotes translation restart after dormancy published in Nature!! This was, as usual, a wonderful collaborative effort between our group and the Mattei Lab @simonemattei.bsky.social @embl.org www.nature.com/articles/s41...

Please share widely. The new (simpler) MIRA R35 for Early Stage Investigators (ESI) NOFO is published. First due date is 10/2/2026. files.simpler.grants.gov/opportunitie...

www.nature.com/articles/s41...

The regulation, function and disease relevance of cytoplasmic tRNAs - Nature Reviews Molecular Cell Biology

This Review discusses the expression and maturation of human cytoplasmic tRNAs and recent insights into their regulation of translation. The authors also explore emerging roles of tRNAs in ribosome-as...

nature.com

An in-press version of our latest paper in @natcomms.nature.com on phospho-activated protein-protein interactions with ssDNA-binding protein RPA during BIR. If your favorite pathway involves RPA, this may be for you! Thanks to the lab, collaborators & @ukri.org! www.nature.com/articles/s41...

This is a really important paper in an area that is hard to study. I would recommend any student read this preprint to see how confounding issues arise even in clever assays. Internal Ribosome Entry Sites (IRES) are very interesting because they bypass normal translation initiation mechanisms. 1/

McManus Lab at CMU@mcmanuslabrna.bsky.social · 4mo ago

Check out our new preprint on IRES studies (www.biorxiv.org/content/10.6...). We show a recently proposed IRES “toolbox” results in mRNA annotation errors and false-positive IRES activities. We provide an alternative set of robust approaches. (1/4)

Check out the final version of this work, out today! @natcomms.nature.com www.nature.com/articles/s41...

Ahmad Jomaa@jomaalab.bsky.social · last yr.

Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...

"Here, we discover that giant DNA viruses encode a distinct and functional IF4F translation-initiation complex to drive protein synthesis, thereby blurring the line between cellular and acellular biology" www.cell.com/cell/fulltex...

Giant DNA viruses encode a hallmark translation initiation complex of eukaryotic life

Giant DNA viruses encode a cap-binding complex homologous to eIF4F, the defining translation-initiation complex of eukaryotes. The viral cap-binding complex is required for viral protein synthesis and...

cell.com

new data from NIH about funding trends through FY2025 also a flyby analysis from Claude lol grants.nih.gov/news-events/...

www.science.org/doi/10.1126/...

Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes

Cells down-regulate protein synthesis when stressed to conserve energy and shift resources toward repair. We found that in some mammalian cells, including neurons, stress also resulted in the formatio...

science.org

That thrill and excitement when a new idea formulates and can’t wait to test it out without knowing what really lies ahead (spoiler- it’s a dragon - it’s always a dragon!)

a man jumping over a fence with the words we 're going on an adventure written below him

ALT: a man jumping over a fence with the words we 're going on an adventure written below him

media.tenor.com

AlphaFold modeling & new assays for mitotic kinase activation in E. coli (#MITOKINAC) reveal molecular basis of specific Aurora A & PLK1 activation through Bora during mitotic entry Lionel Pintard @ccdlab.bsky.social et al #RefereedPreprint ℅ @reviewcommons.org link.springer.com/article/10.1...

Molecular basis for the activation of Aurora A and Plk1 kinases during mitotic entry - The EMBO Journal

The evolutionarily conserved, intrinsically disordered protein Bora is critical for initiating the activation of mitotic kinases. Once phosphorylated at Ser112 by Cyclin A-Cdk1 kinase, phospho-Bora ac...

link.springer.com

Interested in direct tRNA-seq by Nanopore? We developed a new RNA-based barcoding aproach for seq parallelization; it is also cost-saving. Scripts available on github. #tRNA #Nanopore track.smtpsendmail.com/9032119/c?p=...

ADAM-tRNA-seq: an optimized approach for demultiplexing and enhanced hierarchal mapping in direct tRNA sequencing

Abstract. Transfer RNAs (tRNAs) play an essential role in protein synthesis and cellular homeostasis, and their dysregulation is associated with various hu

track.smtpsendmail.com

I think some folks might be happy to see this. NIGMS MIRA R35 for EI/NI has been published. PAR-26-121 files.simpler.grants.gov/opportunitie...

This was another fun collaboration with @professorantony.bsky.social and big thanks to lead authors (Poonam Roshan, Ayush Mistry and Vikas Kaushi) and to all the co-authors. www.nature.com/articles/s41...

Good news- they are continuing to do administrative supplements but the specific dates for NIGMS are unclear!

Updated guidance on NIH grant application due dates (10/1/2025-12/5/2025), along with some other Info. grants.nih.gov/grants/guide...

POSTDOCTORAL POSITION IN STRUCTURAL BIOLOGY We need a structural biologist to work on several CryoEM datasets. For more information, please visit: www.antonylab.org Expertise in structure refinement and model-building are required. Please share.

ANTONY LAB SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE

Edwin Antony Lab Website

antonylab.org

Science People: We at NSF are still recovering/catching up/getting our lives together. But the agency posted these FAQs about post-shutdown resumption of operations which might answer a lot of Qs for you: www.nsf.gov/resumption-o...

Resumption of Operations at NSF

Information for NSF staff and the research community regarding the agency's resumption of operations after a lapse in appropriations.

nsf.gov

We have written two reviews providing a practical guide and fundamentals of cell migration: (1) How to select an optimal cell migration assay, (2) How to analyze cell migration data. We also added a perspective on AI methods of analysis. Coming out soon in Nature Methods.

Want to know how homologous recombination defects are caused upon loss of BRCA2? Check out our recent efforts in uncovering this phenomenon just out in Science! www.science.org/doi/10.1126/...

FIGNL1 inhibits homologous recombination in BRCA2 deficient cells by dissociating RAD51 filaments

Homologous recombination (HR) deficiency upon Breast Cancer Gene 2 (BRCA2) loss arises from defects in the formation of RAD51 nucleoprotein filaments. We demonstrate that loss of the anti-recombinase ...

science.org

Among the anti-recombinases, FIGNL1 rules them all. So much that inactivating it brings BRCA2-deficient cells to life. Who is responsible for RAD51 loading without BRCA2/FIGNL1, check out the paper to find out! Great collaboration with @raychaudhurilab.bsky.social www.science.org/doi/10.1126/...

I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.

See our paper “Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1”. Using biochemistry, we show how DNA incisions by the MutLγ nuclease can lead to expansions, and how expansion is prevented by FAN1. Well done Issam and Valentina! www.nature.com/articles/s41...

Beautiful reconstitution of amino acid stress-dependent ISR activation by my @harvardcellbio.bsky.social colleagues presenting a unifying mechanism for GCN2 activation, which requires ribosome collisions and is enhanced by cognate uncharged tRNA in the A site! www.science.org/doi/10.1126/...

GCN1 couples GCN2 to ribosomal state to initiate amino acid response pathway signaling

During nutrient deprivation, activation of the protein kinase GCN2 regulates cell survival and metabolic homeostasis. In addition to amino acid stress, GCN2 is activated by a variety of cellular stres...

science.org

Emmanuel Levy @elevylab.bsky.social has joined BlueSky 🌟 with a fantastic Cell paper with Shu-ou Shan, showing the interactome of the TOM complex and how cotranslational mito import prioritizes large globular domains. Beautiful science! Give him a warm welcome 🎉 link: www.cell.com/cell/fulltex...

Principles of cotranslational mitochondrial protein import

Selective ribosome profiling reveals that nearly 20% of mitochondrial proteins in human cells are imported during translation on cytosolic ribosomes. Cotranslational import requires an N-terminal pres...

cell.com