The STRAIGHT-IN (Dual) kit is now available via @addgene.bsky.social! 🧬 A complete 24-plasmid toolkit for installing up to two orthogonal CLYBL landing pads, DASIT-based enrichment without FACS, and efficient large DNA integration in human cells. Link: www.addgene.org/kits/gallowa... 🧵

⭕️ The STRAIGHT-IN dual plasmids are now available on Addgene! 🧬 @addgene.bsky.social A full STRAIGHT-IN kit with 20+ plasmids, including everything needed to set up and run the platform, is coming soon 🔥 Stay tuned 🚀

Addgene@addgene.bsky.social · 3mo ago

Need a solution for multi-transgene integration? Check out the STRAIGHT-IN Dual platform! Thanks to the Galloway and Davis Labs for sharing the plasmids: www.addgene.org/browse/artic...

Post 1/3 🧵 Our latest work is out at @natbiomedeng.nature.com!🚀 We present STRAIGHT-IN Dual — a platform for precise, allele-specific integration of two large DNA payloads into human iPSCs, enabling more sophisticated control of complex genetic programs. Link📝: www.nature.com/articles/s41... 👇

💸 Stem cell culture that’s ~95% cheaper and weekend-free: a win-win for PIs and trainees! Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1... Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!

Our review, "Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction." doi.org/10.1038/s415..., has just been published in npj Regenerative Medicine!

Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction - npj Regenerative Medicine

npj Regenerative Medicine - Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction

doi.org

Created a second stem cell biology starter pack, as the first one was full. Let me know if you would like to be added! go.bsky.app/UG3eYwU

How to fix a broken #heart 💔->❤️ #Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes. Happy #Fluorescencefriday #BlueSky & #CardioSky! @ablanchasensio.bsky.social 👀 #Science 💥🧪 #Microscopy 🔬

With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄! This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬 @benjaminbjohnson.bsky.social?

There are so many #morphogenesis researchers here! My first starter pack is full! Here is Part 2: go.bsky.app/K6YYKeC #devbio #cellbio #science Tell me if I should add you but try to make sure, that you're not already in Part 1. I cannot cross-reference everyone 😅 Part 1: go.bsky.app/RzDD9or

New starter pack for all people interested in Gene Editing & Gene Therapy 🧬✂️ including Tools & Delivery|CRISPR-Cas|Zinc finger nucleases|Viral vectors|Non-viral delivery methods go.bsky.app/8R4dVez

Created a starter pack focusing on disease modeling in a dish using stem cells! 🧪🔬 Please (self)nominate anyone you think should be added to the list and repost so it finds the right people ❤️ hPSC Disease Modeling: go.bsky.app/EpWsxNG For a good general stem cell pack go here: go.bsky.app/UTW4d7b

Working on a #StarterPack to collect researchers using human biology-based tools ( #organoids, #microphysiologicalsystems, other human-derived #invitro tools) + computational models & the orgs & accounts that promote non-animal science. Small so far, but I'm new here. Msg to add. go.bsky.app/SVWGwgu

Dedicating this #Fluorescencefriday to the #ECMatrix lovers. Was trialing some ECM staining methods on a scratch wound and love the HABP staining looking almost like flames 🔥🧪🔬 Lets see what you got, @ablanchasensio.bsky.social 😉

Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social

My colleague @benjaminbjohnson.bsky.social has just created a starter pack for hPSC disease modeling. Check it out! 🧪🧫

Benjamin B Johnson@benjaminbjohnson.bsky.social · 2y ago

Created a starter pack focusing on disease modeling in a dish using stem cells! 🧪🔬 Please (self)nominate anyone you think should be added to the list and repost so it finds the right people ❤️ hPSC Disease Modeling: go.bsky.app/EpWsxNG For a good general stem cell pack go here: go.bsky.app/UTW4d7b

🔥Super excited to drop our recent preprint🔥 biorxiv.org/cgi/content/... Here, we automated the generation of 3D Cardiac Microtissues and performed a small high-throughput screen to identify several novel anti-arrhythmic compounds for CPVT1 🧪❤️ #Cardiosky #Science #hiPSC #Arrhythmia #Stemcell

We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs. You can check out our pre-print here: tinyurl.com/27p7hyt9, and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...

Hey #BlueSky! Here is my first #FluorescenceFriday post! This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬. 🔵 Cardiomyocytes 🔴 Endothelial cells 🟡 Fibroblasts More coming next week! 🧪 #Science #Biology #CellBiology #SciArt @benjaminbjohnson.bsky.social

Still a few spots left in the Mammalian Synthetic Biology starter pack!🧪

Albert Blanch-Asensio@ablanchasensio.bsky.social · 2y ago

Please share! Here is a starter pack dedicated to Mammalian SynBio! It is still incomplete so if you would like to be added/removed please let me know! go.bsky.app/SsxHKoV

I thought it would be a good idea to create a starter pack for Engineered Living Materials so the community can grow and post in this platform. Please share and feel free to contact me or comment if you want to be added (or removed)! go.bsky.app/EKRq4zp

Hello #BlueSky! Figured my first post had to be a #FluorescenceFriday 🧪 I work on #hiPSC cardiac disease modeling ❤️ Image shows the initial collision and fusion of a #Cardioid (right) with a blood vessel organoid (left) as part of a fun collaboration with @mvilacuenca.bsky.social!

Please share! Here is a starter pack dedicated to Mammalian SynBio! It is still incomplete so if you would like to be added/removed please let me know! go.bsky.app/SsxHKoV

Here is a starter pack dedicated to fluorescent probes, sensors and fluorophores makers. I'm sure it's incomplete, please reply to this post to be added or suggest someone. go.bsky.app/MtgJE4w

I am really enjoying the surge of people to bluesky but still kind of missing the VERY early career group of people! (Aging in academia is a funny thing) - I went ahead and made this starter pack go.bsky.app/Jq8YaQM - if you would like to be removed/added please let me know

I love how the scientific community here is growing. Here, I started a list of regenerative biologist: go.bsky.app/MGXL9Ra

Couldn’t find a dedicated stem cell biology starter pack, so started one here: go.bsky.app/UTW4d7b Feel free to repost/share and let me know if you would like to be added. #stemcell #pluripotency

⚠️THREAD OF ALL SCIENCE-RELATED STARTER PACKS! ⚠️ 🔄Share to help people finding its topic/field - STRUCTURAL BIOLOGY Structural Biology 1: bsky.app/starter-pack... Structural Biology 2: bsky.app/starter-pack... Crystallography: bsky.app/starter-pack... Synchrotron&CryoEM: bsky.app/starter-pack...

If you're interested in systems biology, check out the Systems Biology Starter Pack! Contact me if you'd like to be added. go.bsky.app/7P14HFu

Started creating a synthetic biology starter pack. Still missing many so reply here if I forgot you or someone else. go.bsky.app/SjfpxTK #synbio

Preprint alert❗ STRAIGHT-IN Dual, a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells. This platform allows the generation of genetically-modified hiPSC lines in 1 week with 100% efficiency! doi.org/10.1101/2024...

STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells

Targeting DNA payloads into human (h)iPSCs involves multiple time-consuming, inefficient steps that must be repeated for each construct. Here, we present STRAIGHT-IN Dual, which enables simultaneous, ...

doi.org