Chlanda lab
@chlandalab.bsky.social
Membrane Biology of Viral Infection cryo-ET, cryo-CLEM, cryo-FIB, influenza, Ebola virus, SARS-CoV-2
In new study led by @bdadonaite.bsky.social, we show many influenza HAs (H5, H7, H9, H1, H2, H3) can use avian or human MHC-II to enter cells. We then use novel combo of deep mutational scanning & cryoEM to define how H5 HA binds to avian MHC-II. Preprint: doi.org/10.64898/202...
Join us at the 24th Workshop Cell Biology of Viral Infections @gesvirologie.bsky.social, Schöntal Monastery, 28–30.10.2026 cellviro.g-f-v.org “Cellular Routes and Roadblocks in Viral Genome Trafficking” Speakers: @beatesodeiklab.bsky.social · Alba Diz-Muñoz · @carogoujon.bsky.social · Redmond Smyth
Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)
Happy to see the preprint of our VULCROM setup online! Thanks to our amazing collaborators we've managed to get some pretty cool cryo-CLEM images by combining cryo super-resolution and cryo-ET: doi.org/10.64898/202...
Big news for correlative cryogenic SMLM/ET! With my colleague Soheil, we (Mahamid and @jonasries.bsky.social labs) indicate lamella-specific illumination can allow 500-1500x higher laser intensities, opening massive new possibilities for correlative imaging #teamtomo www.biorxiv.org/content/10.6...
First steps into cryo-lamella imaging by cryo-secondary ion mass spectrometry using helium and neon ion microscopy! We look forward to continuing with correlative cryo-EM-SIMS. Great work: Antje Biesemeier and colleagues - Luxembourg Institute of Science and Technology. pubs.acs.org/doi/10.1021/...
Cryo-HIM-SIMS on the npSCOPE: Correlative Topographic, Transmitted and SIMS Imaging at Cryogenic Temperatures
Multimodal helium ion microscopy (HIM) and secondary ion mass spectrometry (SIMS) provide in situ spatial imaging and chemical analysis at nanoscale resolution. Operation at cryogenic temperatures is ...
pubs.acs.org
Excited to advertise a fully funded PhD with @socialinfluenza.bsky.social investigating how influenza virus evolution differs between different avian hosts (and how this impacts pandemic potential). Joint between @pirbrightinst.bsky.social + @cvrinfo.bsky.social www.findaphd.com/phds/project...
FULLY FUNDED PhD - Visualising avian influenza virus tropism and co-infection in avian hosts at University of Glasgow on FindAPhD.com
PhD Project - FULLY FUNDED PhD - Visualising avian influenza virus tropism and co-infection in avian hosts at University of Glasgow, listed on FindAPhD.com
findaphd.com
Congratulations to Dao Thi lab @daothilab.bsky.social and collaborators! Beautiful work! @ciid-heidelberg.bsky.social
Virologie Bochum@mmv-rub.bsky.social · 4mo ago🔬 New insights into hepatitis E virus replication 🔬 Study reveals HEV replication occurs in proliferative cells within the intestinal crypt. Read more: www.science.org/doi/10.1126/... #HEV #HepatitisE #Research #Virology #RUB #HepEHub
Meet our new #AllianceFellow Jack Whitehead! The short interview about his work in the @chlandalab.bsky.social and Mahamid lab at @embl.org can be found on our LinkedIn account. @jackwh18.bsky.social
Meet our new Alliance Fellow Jack Whitehead! Q: Could you tell us a bit about the work you do? Which field do you work in and who are your PIs? A: I am a structural biologist, and my work looks at… |...
Meet our new Alliance Fellow Jack Whitehead! Q: Could you tell us a bit about the work you do? Which field do you work in and who are your PIs? A: I am a structural biologist, and my work looks at th...
linkedin.com
We found a viral Trojan Horse: a virus can hide inside another virus.This one surprised us: deltaviruses don’t just borrow a helper virus. They can travel inside it. A literal Trojan Horse “virus-in-a-virus” route into cells. 🤯 Kudos to 1st author @viroscope.bsky.social and co-authors !
Deltaviruses spread through a viral Trojan Horse
Hepatitis D-like satellite viruses, known as deltaviruses, have been recently discovered in a wide range of animals. These viruses are thought to expr…
sciencedirect.com
Our latest preprint is now available doi.org/10.1101/2025...! Led by my postdoc, Luqiang Guo @luqiang.bsky.social, we determined a ~2.4 A resolution structure of the hantavirus glycoprotein tetramer on the surface of VLPs. Also determined structures of dimers of tetramers and Fab-bound tetramers.
Afterwards a panel discussion was moderated by Caroline Schlaufer featuring film director Christian Frei, biosafety expert Kathrin Summermatter & virologist @profvolkerthiel.bsky.social. The audience posed great questions on funding, biosafety & media polarisation.
📢Job alert📢 @uniheidelberg.bsky.social has an opening for a full W3-position on “Modeling of Biological Processes” 😀👍 Here is the call tinyurl.com/3w9e9dtf and application deadline is March 15. The group would be located at the beautiful #BioQuant center right in the middle of Neuenheimer Feld.
Trapozoid cryo-FIB milling allows to prepare a lamella for dual-axis cryo-ET and assessment of different deep-learning missing-wedge restoration algorithms. rdcu.be/e2nXE
Ulrich Schwarz@ulrichschwarz.bsky.social · 6mo ago📢Paper alert📢 Now out in @bmc.springernature.com with open access: our very cool collaboration with @chlandalab.bsky.social on FIB-milling designs for cryoET rdcu.be/e2nXE Here you can see different designs for the stress relief cuts for dual-axis tomography. Read the paper for more details😀
New Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
Nature Protocols, Published online: 30 January 2026; doi:10.1038/s41596-025-01284-zA protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian cells, as well as primary Drosophila melanogaster neurons.
dlvr.it
Check out our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3) 🔗 biorxiv.org/content/10.6...
Cryo-EM structure reveals how influenza A virus NEP binds the viral polymerase at a regulatory hotspot, coordinating RNA synthesis and nuclear export. Fantastic collaboration with @loiccarrique.bsky.social and Jon Grimes. www.science.org/doi/10.1126/...
I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look! #virology #teamtomo www.nature.com/articles/s41...
Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation - Nature Communications
In this study, Dahmane et al use a method called cryo-electron tomography to uncover new details of how tick-borne flaviviruses transform cells into virus factories.
nature.com
Spillover of #influenza A viruses from animals to humans represents a threat to our health. In this Perspective, Silke Stertz @virology.uzh.ch discusses emerging research that suggests some influenza A viruses can enter host cells via MHC-II receptors across species 🧪 #virology plos.io/4pP3Gnq
A new path to spillover: MHC-II entry of influenza A viruses
Spillover of influenza A viruses from animals to humans represents a threat to our health. This Perspective discusses emerging research that suggests some influenza A viruses can enter host cells via…
journals.plos.org
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems. For more details and to apply please see www.imperial.ac.uk/jobs/search-...
Our new preprint is out 🥳🥳🥳 Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence? 👇
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems. For more details and to apply please see www.imperial.ac.uk/jobs/search-...
warwick.ac.uk/fac/cross_fa... I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays. If you're interested reach out and I'd be happy to have a chat.
Dr Jeremy Keown
Dr Jeremy Keown
warwick.ac.uk
Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET! 🔗 rdcu.be/eMmct We are very excited that our paper is finally out! 🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
science.org
Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids. Link: www.biorxiv.org/content/10.1...
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit www.nexperion.net/semcm2026
