Our AI-guided design of RNA-guided nucleases is now out in @science.org 💻🧬 We used structure and evolutionary data to generate minimal, active and divergent gene editors! www.science.org/doi/10.1126/... Congrats to @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social & team!

Structure and evolution-guided design of minimal RNA-guided nucleases

The design of RNA-guided nucleases with properties not limited by evolution can expand programmable genome-editing capabilities. However, generating diverse multidomain proteins with robust enzymatic ...

science.org

Continuous recording beam image shift speeds up single particle and tomographic data collection. www.nature.com/articles/s41...

New paper! How do RNAs "know" where to go inside a cell? We dug into the sequence elements that route RNAs to the right place. It turns out that, in mammals, they're surprisingly massive (>200 nt), multipartite, and wonderfully complicated. 🧵

Excited to share our work uncovering SNOR, a ribosome-associated factor that promotes translation restart after dormancy published in Nature!! This was, as usual, a wonderful collaborative effort between our group and the Mattei Lab @simonemattei.bsky.social @embl.org www.nature.com/articles/s41...

Our lab is proud to present our latest work harnessing Bridge Recombinase for genome-scale editing in diverse bacteria, microbiome editing, and programmable horizontal gene transfer.

bioRxiv Synthetic Biology@biorxiv-synthbio.bsky.social · 3mo ago

Bridge recombinase enables versatile rewriting of bacterial genomes https://www.biorxiv.org/content/10.64898/2026.04.29.721476v1

The best thing since *before* sliced bread... VIPRs. www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6...

"Use it or lose it." This is great advice for exercise, but when cells need to slow down or go dormant, they need to store ribosomes to recover growth in the future. They use hibernation factors to do this. Here is our latest story on how archaea hibernate ribosomes (1/7): doi.org/10.64898/202...

Excited to share our first work on protein design! Huge thanks to the entire team, and especially to Bel, Evan, to the Doudna, Jacobsen, Cate, Banfield labs, all co-authors, and my D-lab mates! 💫

Doudna Lab@doudna-lab.bsky.social · 8mo ago

✨New preprint! 🧵1/4 Excited to share our work on AI-guided design of minimal RNA-guided nucleases. Amazing work by @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social et al! Multi-lab collaboration @banfieldlab.bsky.social @jhdcate.bsky.social @jacobsenucla.bsky.social🧬 🔗👇

A very nice Preview of our work in @cp-cellhostmicrobe.bsky.social this morning from Chrishan Fernando & Nicole D. Marino! www.cell.com/cell-host-mi...

Viral enzymes degrade to evade

Across all domains of life, immune systems rely on nucleotide-based signaling molecules to activate defense responses. In a recent Cell Host & Microbe study, Doherty, Nomburg, and colleagues identify ...

cell.com

Our study from the Steckelberg lab is out now in @narjournal.bsky.social !! We dove into a new nuclease-blocking viral #RNA structure important for viral infection, which provided evidence of similar structure-based strategies across diverse viral families. academic.oup.com/nar/article/...

A conserved viral RNA fold enables nuclease resistance across kingdoms of life

Abstract. Viral exoribonuclease-resistant RNA (xrRNA) structures block cellular nucleases to produce subgenomic viral RNAs during infection. High sequence

academic.oup.com

Archaeal virus entry and egress Archaea also have viruses and they are cool! #microbiology #virology #virus #viruses @femsmicro.org @femsjournals.bsky.social academic.oup.com/microlife/ar...

Archaeal virus entry and egress

Abstract. Archaeal viruses display a high degree of structural and genomic diversity. Few details are known about the mechanisms by which these viruses ent

academic.oup.com

Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work. www.nature.com/articles/s41...

Structural insights into transcriptional regulation by the helicase RECQL5 - Nature Structural & Molecular Biology

Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s ...

nature.com

We are happy to share that our snR30 story is finally out in @natureportfolio.nature.com 🥳 We report the first structure of a H/ACA snoRNP acting in ribosome synthesis thereby providing a detailed structural and biochemical view of the snR30 snoRNP guiding local 18S rRNA subdomain folding. 👇👇👇

Final grad paper is officially out! Grateful for the incredible team and all the support along the way! www.nature.com/articles/s41...

Tick-borne flavivirus exoribonuclease-resistant RNAs contain a double loop structure - Nature Communications

Many flaviviruses generate specialized non-coding RNAs that resist degradation by host exonucleases, promoting viral infection. Here, the authors characterize an RNA element in Powassan virus, highlig...

nature.com

Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides. Check it out:

A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway

Microbial and viral co-evolution has created immunity mechanisms involving oligonucleotide signaling that share mechanistic features with human anti-viral systems. In these pathways, including CBASS a...

biorxiv.org

Giving a seminar for the Genome Engineering Seminar Series at Harvard this Monday, April 7 at 1pm EST / 10am PST! 🎙️ I’ll be talking about our Cas9 work—if you’ve ever wondered what the recipe is for high-efficiency CRISPR genome editing, come hang out!🧬💥 📍 Zoom link: harvard.zoom.us/j/94394339529

Really nice issue of RNA this month featuring many Perspective pieces on RNA in disease. rnajournal.cshlp.org/content/31/3...

We present Rfam-based RNA structural alignments and other structure-based inputs for RNA 3D structure prediction by deep learning. www.biorxiv.org/content/10.1...

Single-molecule live-cell RNA imaging with CRISPR–Csm - @doudna-lab.bsky.social @qb3-berkeley.bsky.social @innovativegenomics.bsky.social go.nature.com/3EJPKsM

Single-molecule live-cell RNA imaging with CRISPR–Csm - Nature Biotechnology

Single-molecule live-cell fluorescence in situ hybridization uses an RNA-targeting CRISPR–Csm complex to image and track endogenous RNAs.

go.nature.com

Latent Labs comes out of stealth today with $50M funding. Our goal? To push the frontiers of generative biology, giving partners instant access to tools capable of accelerating drug design

I had the pleasure of co-hosting this new career exploration podcast from ASBMB- tune in to hear from my fellow committee member Tom Kiselak about being a patent attorney!

American Society for Biochemistry and Molecular Biology@asbmb.bsky.social · last yr.

🎙️ASBMB's new series features interviews with biological and life sciences professionals about their careers! ow.ly/268250UYcmx 🎧Check out episode 1: A conversation w/Tom Kiselak (Thermo Fisher Scientific), who holds a #PhD in analytical chemistry & works as a patent attorney: youtu.be/enBtSSTQT9A.

Excited to see this work finally out, it was a pleasure to be involved!

Innovative Genomics Institute@innovativegenomics.bsky.social · last yr.

New article from @jhdcate.bsky.social and collaborators in @narjournal.bsky.social: RNA elements required for the high efficiency of West Nile virus-induced ribosomal frameshifting doi.org/10.1093/nar/... 🧪🧬🦠

Excited🚨to share our first work on the human gut phageome doi.org/10.1101/2025... Always inspiring to work with @epcrocha.bsky.social , Erick D, Camille d'H and everyone else within this super dynamic consortium @inrae-france.bsky.social+@pasteur.fr+@inserm.fr! Many thanks for this great journey!🙌

Third-generation cephalosporin antibiotics induce phage bursts in the human gut microbiome.

The use of antibiotics disrupts the gut microbiota, potentially leading to long-term health issues and the spread of resistance. To investigate the impact of antibiotics on phage populations, we follo...

doi.org

Today, the IGI turns 10! We’ve grown from a fledgling initiative focused around developing #CRISPR tools to a full-blown research institute bringing together leaders in basic & translational research! ⚕️💊🌱☀️ Take a look at our timeline & key achievements from our first decade: ow.ly/GzVt50UTsbP

A conserved class of viral RNA structures regulates translation reinitiation through dynamic ribosome interactions pubmed.ncbi.nlm.nih.gov/39893634/ #cryoEM

Thrilled to have been involved in this work and looking forward to what the Sherlock Lab has in store!

cryoEM papers@cryoempapers.bsky.social · 2y ago

A conserved class of viral RNA structures regulates translation reinitiation through dynamic ribosome interactions pubmed.ncbi.nlm.nih.gov/39893634/ #cryoEM

✨ New from C-GEM in ACS Central Science: Thioesters Support Efficient Protein Biosynthesis by the Ribosome pubs.acs.org/doi/10.1021/... Congrats to Alexandra & Jacob, along with Isaac, Jess, @alexsolivan.bsky.social, @nxhamlish.bsky.social, Katie, Alanna, Jamie, and Scott! #NSFfunded @pubs.acs.org

Thioesters Support Efficient Protein Biosynthesis by the Ribosome

Thioesters are critical chemical intermediates in numerous extant biochemical reactions and are invoked as key reagents during prebiotic peptide synthesis on an evolving Earth. Here we asked if a thio...

pubs.acs.org

www.cell.com/cell/fulltex...

Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation

Ribosomes cooperate through transient collisions to ensure efficient translation.

cell.com

Out today @ Science: TIR signaling activates caspase-like immunity in bacteria We report a new immune signaling molecule: N7-cADPR. Produced by phage-induced TIRs and activates a defensive bacterial caspase Congrats Francois Rousset, Ilya Osterman and coauthors! www.science.org/doi/10.1126/...

Our paper out @Nature: CARD domains mediate anti-phage defense in bacterial gasdermin systems CARDs are essential for caspase recruitment during human inflammasome activation. We now find them in bacterial immune systems Congrats Tana Wein! Thank you Kranzusch lab! www.nature.com/articles/s41...