Targeting SARS-CoV-2 programmed -1 ribosomal frameshifting: structural dynamics and RNA-directed antiviral strategies pubmed.ncbi.nlm.nih.gov/42545119/ #cryoEM

Structural and functional characterization of a conserved cryptic epitope on SARS-CoV-2 spike S2 subunit pubmed.ncbi.nlm.nih.gov/42546045/ #cryoEM

We are looking for a lab manager starting from January 2027, you can find more information on our members page pacesalab.com#members. We are also starting to look into hosting master students starting 2027.

We are excited to share two preprints reporting a mosquito-specific receptor for chikungunya virus and other alphaviruses! 🤩 www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6... @harvardmicro.bsky.social @harvardvirology.bsky.social @hhmi-science.bsky.social

Great to see this finally published! We describe channels at the poxvirus core wall involved in nucleic acid release. www.nature.com/articles/s41...

In situ structure of the poxvirus portal complex - Nature

Cryo-electron tomography uncovers the structure of a conserved hexameric ‘portal complex’ that spans the core wall in vaccinia virus, and shows how it is associated with viral functions such as a...

nature.com

One month left to apply (deadline August 31st)! 👇 #Virology #AcademicJobs #FacultyJobs #FacultyPosition #TenureTrack #GroupLeader

Daniel Hurdiss@danielhurdiss.bsky.social · last mo.

We are looking for an Assistant or Associate Professor to join us at the @utrechtvirology.bsky.social. Interested in building an independent research programme in a collaborative #virology environment? Please take a look and share with anyone who might be a good fit! www.uu.nl/en/organisat...

One cell, one tomogram! Our montage tomography workflow, MOSAIC, is out in Structure, and MontageMaker is on GitHub! MOSAIC: www.cell.com/structure/fu... MontageMaker: github.com/MPI-Dortmund... Big thanks to @maikaboiero.bsky.social, Adriana Prajica, Gavin Rice, and @raunser-lab.bsky.social!

Targeting Picornavirus Nonstructural 2C Protein: Structure, Function, and Antiviral Discovery pubs.acs.org/aidcbc/artic...

Targeting Picornavirus Nonstructural 2C Protein: Structure, Function, and Antiviral Discovery

Abstract. Picornaviruses cause substantial human and veterinary disease, yet direct-acting antivirals remain limited. The nonstructural protein 2C is among

pubs.acs.org

Thousands of possible receptor-peptide combinations. One AI-powered solution. Led by @lariferg.bsky.social, a study from @schaferlab.bsky.social & Isabel Beets has developed DeorphaNN, a neural network to identify peptide pairings for GPCRs. Read more: mrclmb.ac.uk/news-events/... #LMBResearch

Happy that our most recent paper on nucleotide excision repair mechanisms has now been published in Science Advances. This project was led by @natalia-mg.bsky.social and @callumhaste.bsky.social and focused on visualising early steps of the NER process by #cryoEM. www.science.org/doi/10.1126/...

Visualization of stepwise derepression of TFIIH in global genome nucleotide excision repair

Cryogenic electron microscopy captures nucleotide excision repair complexes assembling on damaged DNA templates.

science.org

A comprehensive overview of how X-ray crystallography, cryo-electron microscopy, micro-electron diffraction and cryo-electron tomography have advanced drug discovery across diverse therapeutic areas is provided #XRayCrystallography #CryoEM #CryoET doi.org/10.1107/S205...

🚀 Can automation solve preferred orientation in your #cryoEM datasets? After automating processing across 21 GPCR datasets in #CryoSPARC, we updated the workflow to be even more robust to preferred orientation, achieving improved map quality over manual processing. Read more 👉 bit.ly/3RyOIGG

Direct lipid interactions control SARS-CoV-2 M protein conformational dynamics and virus assembly www.nature.com/articles/s41...

Here's an interesting one: S:P812R in a BA.3.2 from Western Australia—creates a furin cleavage site (FCS) in the S2' cleavage position S:685. The classic SARS-2 FCS is at S:682-685. Spike gets cleaved by furin there before it leaves the cell (usually). The S2' cleavage site is different... 1/4

Thrilled to share our collab led by Grace Hibshman with the Shipman lab! Type IX retron-Kva2 structure reveals phage detection via an HTH fold, not a primary sequence. We computationally designed triggers that program bacteria cell death, pointing toward designer antimicrobials.

Really looking forward to visiting ISTA in just over two months for the 9th Austrian #CryoEM Symposium! Excited to give an EMBO YIP Lecture, hear from a great line-up of speakers, and catch up with the cryo-EM community. Hope to see you there! www.imp.ac.at/research/cry...

Confidence-guided cryo-EM map optimisation with LocScale-2.0 pubmed.ncbi.nlm.nih.gov/42469226/ #cryoEM

Structure and dynamics of the Nipah virus RNA-dependent RNA polymerase pubmed.ncbi.nlm.nih.gov/42469089/ #cryoEM

1/5: Our latest preprint is out! We show that LEA proteins can diversify particle orientations in cryo-EM, helping to overcome preferred orientation at the air-water interface (AWI). www.biorxiv.org/cgi/content/... with @cijilim.bsky.social and Tim Grant #CryoEM #LEAproteins

Diversifying particle-orientation distributions in cryo-EM with LEA protein additives

Preferential orientation at the air-water interface (AWI) remains a persistent challenge in cryo-electron microscopy, often requiring extensive optimization or specialized grid preparation strategies....

biorxiv.org

@emdatabank.bsky.social EMPIAR entry for stomatin complex from @fravallese.bsky.social and myself released - I hope it will be useful for folks studying #cryoem compositional heterogeneity in pseudo/mixed symmetry systems, as classifying out AQP1 and UT-B was quite a challenge for us at least!

Some progression on 58 kDa GBA as part of my work with @recon4imd.bsky.social. Combined tilted data (Glacios) with careful processing gives a ~4.0 Å map with clear secondary structure features. Still not the resolution that I need for variant studies but a step in the right direction! #CryoEM

🧬 Thrilled to share our latest @biorxiv-microbiol.bsky.social preprint! Here we find that Retron-IX build crazy supramolecular rings scaffolded by DNA molecules that sense phage infection! www.biorxiv.org/content/10.6... Read more below🧵! (1/X)

Structural basis of biofilm formation mediated by the Pseudomonas aeruginosa fibrillar adhesin CdrA led by @olivia--smith.bsky.social Collaboration with @alexbateman1.bsky.social , Andres Floto and @geiselbiofilm.bsky.social labs

Super-resolution atlas of SARS-CoV-2 infection reveals protease-dependent organelle maturation, dsRNA landscapes, and intracellular structural proteins www.nature.com/articles/s41...

Reprocessed an old Glacios dataset and pushed this Fab fragment to 3.8 Å resolution! Not a record-breaker by today’s standards, but still pretty satisfying for a C1 particle with only ~48 kDa of ordered mass. #cryoEM

Read our new paper! We show how the protease BepA binds the BAM complex and undergoes striking conformational changes, embedding its water-soluble lid into the membrane to cleave outer membrane proteins. www.nature.com/articles/s41... #cryoEM #astburycentre

Online Now: A conserved mechanism of membrane fusion in nuclear pore complex assembly Online now:

A conserved mechanism of membrane fusion in nuclear pore complex assembly

Fischer et al. uncover a fundamental mechanism of membrane fusion during nuclear pore complex (NPC) assembly. Brl1 and Brr6 form ring-shaped, membrane-remodeling complexes that interact and bridge the nuclear envelope at NPC-assembly sites to mediate fusion. This pathway is conserved in humans via the metazoan fusogen CLCC1.

dlvr.it

Highly pathogenic avian influenza H5 detected in a greater crested tern at Robe, Limestone Coast, South Australia. All previous detections in Australia were in migratory birds that had likely brought the disease from the subantarctic, but the greater crested tern is a resident seabird in Australia.

The Guardian@theguardian.com · 4w ago

Deadly H5 bird flu found in local Australian seabird for first time

Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion @science.org @harvardmed.bsky.social www.science.org/doi/10.1126/...

Structure and receptor recognition of type-II feline infectious peritonitis virus spike glycoprotein pubmed.ncbi.nlm.nih.gov/42420488/ #cryoEM