New preprint! 🚀 We introduce AbTags – antibody–peptide fusion proteins that massively amplify signal, enable absolute quantification of binding events, and are read out together with the single-cell proteome by LC–MS. AbTag platform is patent-pending. Preprint: www.biorxiv.org/content/10.6...

Antibody-based signal amplification for single-cell proteomics by mass spectrometry

Single-cell proteomics by mass spectrometry (scp-MS) detects thousands of proteins per cell, yet low-abundant proteins routinely detected by antibody-based methods are challenging to quantify in the s...

biorxiv.org

Quick heads up - there will soon be a job posting for a Staff Scientist / Senior Staff Scientist in our lab in Copenhagen to support all our mass spec proteomics adventures! Great colleagues, lots of cool projects… and you’ll get to work with me 😅 Please DM if you’re interested!

This looks like a fantastic resource to study human kinase signalling. So much MS instrument time.

bioRxivpreprint@biorxivpreprint.bsky.social · 9mo ago

Chemical proteomics decrypts the kinases that shape the dynamic human phosphoproteome https://www.biorxiv.org/content/10.1101/2025.11.18.689017v1

NEW Researcher Profiles in Altmetric Altmetric tracks research items and where they are mentioned or cited online. Searching by researcher name wasn't a feature unless you imported your own from a repository or CRIS Today we are launching a global researcher search feature. 1/5

@kentsisresearch.bsky.social develop a proteogenomics workflow to detect 1000s of unknown cancer proteins. PG3 combines long-read RNA sequencing with multi-protease MS to discover non-canonical proteins in cancer cells. Used a timsTOF HT, nanoElute 2 & Aurora Ultimate 25x75 column: bit.ly/4mlQSmu

When testing a computational data analysis method: simulated data are good for checking whether the method is doing what you think it should be doing, real data for checking whether what you think it should be doing is any good.

Are Solid Particles Ready for Prime-Time Proteomics? pubs.acs.org/doi/10.... --- #proteomics #prot-paper

Start of the European symposium in single cell proteomics at beautiful Viena with a workshop on spatial proteomics organised by @coscialab.bsky.social

Mapping early human blood cell differentiation using single-cell proteomics and transcriptomics www.science.org/doi/... --- #proteomics #prot-paper

Only a few more hours! 😃

@kmechtler.bsky.social · last yr.

The European Symposium on Single Cell Proteomics (ESCP) will be held in Vienna for the 6th time, from August 26th – 27th 2025 This year's focus will include spatial proteomics studies on the single-cell level. There is no registration fee Hotel rooms in Vienna are inexpensive. www.apma.at/6thescp/

We’re excited to present this integrative analysis of single-cell proteomics and transcriptomics of the human HSPC hierarchy. Now published in @science.org together with @fabiantheis.bsky.social, @erwinschoof.bsky.social, @porsebo.bsky.social. www.science.org/doi/10.1126/... 🧵

Mapping early human blood cell differentiation using single-cell proteomics and transcriptomics

Single-cell transcriptomics (scRNA-seq) has facilitated the characterization of cell state heterogeneity and recapitulation of differentiation trajectories. However, the exclusive use of mRNA measurem...

science.org

Here’s an interesting story for #teammassspec. Liraglutide went generic in December 2024 and we have been developing methods for impurity characterization but we kept seeing an unusual modification with a +12 Da shift. A little digging revealed that others had noticed that the n-terminal histidine..

Pumped to finally show off our spatial proteomics work presented a few weeks ago at the EAS Congress @eassociety.bsky.social and now live on @biorxivpreprint.bsky.social 🥳 Time for a science thread! #TeamMassSpec #SpatialProteomics #AtheroSky 🧵 www.biorxiv.org/content/10.1...

Tried presenting my data at #EuPA2025. Then decided to scale things up... Turns out, volcano plots look great in sand and people are more interested in what we scientists do. #Proteomics #YPIC #YPIContest2025

The reports of Bluesky’s death have been greatly exaggerated

Altmetric@altmetric.com · last yr.

The trend is still in Bluesky's favour. X is still larger but very regularly Bluesky easily eclipses it in terms of research being shared. Linda Yaccarino claimed X had 600m users in April. Bluesky has 36m accounts created in total.

The financial crisis of the HE sector in the UK now leads to increased numbers of grant and fellowship applications, pushing down the success rates. Since also overheads increased, in some cases we will see success rates halved. There is a BBSRC fellowship which will have a success rate of 5%.

On my way home from what was an absolute blast of a EuPA2025 in the lovely Saint-Malo in France. So much great science, amazing people, old & new friends, and not the least, our valuable tech partners. The future of proteomics is clearly still only beginning & we all seem to be enjoying the ride! :)

The year of "How deep can we go?" Erwin Schoof presents a view of the deepest proteome coverage across various cell types at #EuPA2025, using the newly released Orbitrap Astral Zoom. Analysing 40 HEK2983T cells per day, using an Aurora Ultimate 25x75 will consistently get you ~6,000 protein IDs.

Our amazing group of experts: Jenn Abelin, @berndwollscheid.bsky.social, @erwinschoof.bsky.social , Tim Van Den Bossche and Stefanie Hauck 👏 If you would like to join, please register using the QR code or this link: docs.google.com/forms/d/e/1F... #Proteomics #EuPAFPS2025 #EuPA2025 #SaintMalo

Still figuring out which evening workshop to attend tonight at #ASMS2025? Come on by Ballroom II and hear about hands-on tips and tricks for one of the hottest and fastest developing areas within LC-MS based proteomics, namely single-cell. 😃

WE ARE RECRUITING: A tenure-track group leader position in translational cancer research is available at the Finsen Laboratory in Copenhagen. Come join an excellent and friendly research environment combining both hospital and university affiliated groups candidate.hr-manager.net/ApplicationI...

Tenure-track Research Group leader position in translational cancer research at the Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital

A group leader position at the Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital is open for appointment as soon as possible. The position is av

candidate.hr-manager.net

#ASMS2025 offers great evening workshops. If you are interested in real-time decision-making during MS data acquisition or still looking for one to join, consider the Real time Mass Spectrometry Workshop featuring speakers and panelists Sarah Sipe, Aarthie Senathirajah, and Manuel Peris Diaz.

#ASMS2025 Come here about the latest in real-time instrument control including applications to single cell proteomics (@parallelsq.bsky.social), #glycotime, and quantitative proteomics!

Nick Riley@nmriley.bsky.social · last yr.

#ASMS2025 offers great evening workshops. If you are interested in real-time decision-making during MS data acquisition or still looking for one to join, consider the Real time Mass Spectrometry Workshop featuring speakers and panelists Sarah Sipe, Aarthie Senathirajah, and Manuel Peris Diaz.

I am really pleased we managed to squeeze this story out during #ASMS2025. This is our contribution relating to the fantastic Omnitrap/Orbitrap. What we thought would be a straightforward conversation of our exploris-omnitrap turned into quite a journey. (1/n) www.biorxiv.org/cgi/content/...

Integrating Alternative Fragmentation Techniques into Standard LC-MS Workflows Using a Single Deep Learning Model Enhances Proteome Coverage

We built and characterised a mass spectrometer capable of performing CID (both beam type and resonant type), UVPD, EID and ECD in an automated fashion during an LCMS type experiment. We exploited this...

biorxiv.org

New Preprint ... This was a bit different of a project than what we normally do for hardware comparisons. I think there is a lot of potential in the methods mentioned here for calibrating the instrument response to a common scale. Nice work by @chrhsu.bsky.social! www.biorxiv.org/content/10.1...

Evaluation of an Orbitrap Astral Zoom mass spectrometer prototype for quantitative proteomics - beyond identification lists

Mass spectrometry instrumentation continues to evolve rapidly, yet quantifying these advances beyond conventional peptide and protein detections remains challenging. Here, we evaluate a modified Orbit...

biorxiv.org

Evaluation of an Orbitrap Astral Zoom mass spectrometer prototype for quantitative proteomics - beyond identification lists www.biorxiv.org/cont... --- #proteomics #prot-preprint

Yesterday, we fired up the grill at our annual Evosep User Meeting. It was inspiring to reconnect with users and colleagues from the #proteomics community. If you’ve got room for a little more orange in your morning, join us for breakfast tomorrow. www.evosep.com/asms2025/

Thanks for the kind shout out, Ben! 🙏🏻 Hope to catch you in Baltimore!

ProteomicsNews (Ben Orsburn)@proteomicsnews.bsky.social · last yr.

Interested! I'll sure try to swing by! proteomicsnews.blogspot.com/2025/05/info...

Super excited to have this work out & share it at large with the community at #ASMS2025. Jakob came up with a really clever way to integrate targeted&global proteomics, and together with the @porsebo.bsky.social lab, we pushed the boundaries of what we thought biologically possible. Congrats Jakob!🥳

Jakob Woessmann@jwoessmann.bsky.social · last yr.

I am excited to share the first project of my PhD on bioRxiv and just in time for #ASMS2025 ! Increasing the sensitivity in single cell proteomics to quantify transcription factors in single hematopoietic stem and progenitor cells by iDIA. www.biorxiv.org/content/10.1...

The Cell Diversity Lab is well represented at this year's ASMS; please come and talk with Jakob Woessmann and Benjamin Furtwängler to hear all about their latest efforts in improving, and applying single-cell proteomics by MS. #singlecellproteomics #teammassspec #cellheterogeneity