Today I am sharing a new preprint and accompanying blog post. Hundreds of published papers include impossible western blots showing protein expression in cell lines that have deleted or mutated genes. These clumsy mistakes are widespread and repeated by LLMs.

Your monthly reminder that if you're using ChatGPT to do your science, you're failing your colleagues, community, and all of us. Here, ChatGPT makes up data and presents it as the real thing — then apologizes when confronted.

RIP Walter❤️

Paper now out in Nat Comm by the Gatsogiannis group (wit us): Fantastic cryoEM structure of #VCP/p97-Ufd1-Npl4 with FAF1 showing how the accessory adapter coordinates Ufd1-UT3 to feed #ubiquitin into p97. Plus incredibly detailed dynamics of the complex. Thx for a great collaboration.

A good run down for whose fault it is, including the connections with transphobia, in this video (hastily added subs put in by me) www.instagram.com/reel/De369PV...

so as I was saying last month, Oxbridge function as white supremacist strong holds and the latest evidence of this is that Oriel College, Oxford are hosting Nathan Cofnas, who is openly an advocate for eugenicist positions

Chanda Prescod-Weinstein 🌌@chanda.blacksky.app · 2mo ago

I firmly believe that a broad investigation into the way Oxbridge have functioned as white supremacist strongholds and staging grounds for keeping eugenicist discourse alive while simultaneously sustaining functional segregation at all levels would address what happened and do important work beyond

CryoEM structures of human NHE6 and NHE9 - Na⁺/H⁺ exchangers that help fine-tune endosome pH. Excited to be a part of this study lead by @chembiohub.bsky.social, Jesper Hansen, and Ashley Pike with Gamma Chi, Giulio Superti-Furga, @madamescientist.bsky.social and Bayer doi.org/10.1038/s414...

High-resolution structures of human NHE6 and NHE9 elucidate endosomal ion and lipid interactions - Nature Communications

NHE6 and NHE9 regulate endosomal pH and are linked to neurological disease. Here, the authors report cryo-EM structures of human NHE9 and two NHE6 splice isoforms, revealing sodium and lipid- binding ...

doi.org

interesting details here on the data processing used to get the small particle to high resolution, including the use of HR-HAIR

cryoEM papers@cryoempapers.bsky.social · 5d ago

Cryo-EM structure of the sub-50 kD PDZ-RhoGEF catalytic core-RhoA complex reveals an allosteric mechanism for GEF switch www.biorxiv.org/content/10.64898/2026.10.21.760610v1 #cryoEM

Open position! ❄️ The @irmaquerques.bsky.social Lab is looking for an experienced researcher with a strong practical background in cryo-EM ➡️ tinyurl.com/mr22fkty

The greatest challenge of our time isn’t to figure out how to get to Mars or become interstellar, but to figure out how to live sustainably on Earth.

This is dumb.

Cato Institute@cato.org · last wk.

Most billionaire wealth isn't in yachts or jewelry — that's just a few percent of it. Most is productive business capital: companies that employ people, build things, and fund research. Tax that wealth and you're taxing the businesses that employ us all. Cato's Adam Michel explains.

It is done. My magnum opus. My legacy.

RIP to this angel

Great opportunity here! #CryoEM

Harry Low’s Lab @lowlab.bsky.social · last wk.

💥 Post-doc opportunity! 💥 Come join our team (www.thelowlab.org) at Imperial, London, working on the cryo-EM structure and mechanism of bacterial secretion systems. 48 months. Please RT. For more details and to apply: www.imperial.ac.uk/jobs/search-...

🍩!

Homer Simpson: Mmm... Donut

ALT: Homer Simpson: Mmm... Donut

static.klipy.com

Luuk Loeff@lloeff.bsky.social · last wk.

Exciting times for cryo-EM in NL 🇳🇱! As part of EMPower, two new Titan Krios G5 microscopes have been installed at NeCEN in Leiden and Utrecht. First benchmarks with ApoF reached 1.12 Å resolution 🤯. Looking forward to seeing all the exciting science these microscopes will enable! 🔬

Unfortunately I am back on the job hunt (current position ending due to budget). I'm looking for a position preferably involving plant or fungal biology and of course anything microscopy related. Willing and able to move almost anywhere.

Together, we’re suing the National Institutes of Health; NIH Director Jay Bhattacharya; United States Department of Health and Human Services; HHS Secretary Robert F. Kennedy Jr., and the United States DOGE Service. 16/

Together with other scientists I’m suing the 🇺🇸 US National Institutes of Health for violating our 1st Amendment rights & censoring our health science. I’m ready to shine a light on the darkest days. Buckle up, read on. This gets pretty wild… THREAD

ACLU@aclu.org · 2w ago

By terminating research grants like Kelton's to censor views it doesn’t like, the NIH is blocking scientists from work that would advance public health for all of us. We're pushing back. Tell Congress to demand the NIH restore the grants and stop its censorship campaign at aclu.org/nih_censorship_

Our AM-3 paper is out in Cell. Opioids are indispensable therapeutics, but have severe side effects. Because they are administered systemically, it is hard to dissect how they work, and to separate side effects. So we built one you can switch on and off with light. 🧵 www.cell.com/cell/abstrac...

Just make it exist first, you can make it good later.

5/5 A dream collaboration 🎉🥳, with theory and experiment hand in hand 🙌 with Deb Maji on the experiments, @steve-bonilla.bsky.social , Alex de Marco @rocovino.bsky.social labs, @larsdingeldein.bsky.social @minhuanli.bsky.social computational front. Check it out: www.biorxiv.org/content/10.6...

Quantifying RNA Folding Equilibria from Unclassified Cryo-EM Particle Images

Functional RNA structures are typically far more dynamic than most proteins solved by cryo-electron microscopy (cryo-EM). Flexible junctions and weak tertiary contacts produce broad conformational ens...

biorxiv.org

Our paper on the #CsMT workflow to reconstruct microtubules in #Cryosparc is now online. Fantastic work from several undergrads in the lab and Asuva Arin, and fantastic collaboration with the Cianfrocco lab. Please try it out; it is simple and quick. #OpenScience doi.org/10.1107/S205...

CsMT: a robust and streamlined CryoSPARC workflow for cryo-EM reconstruction of microtubules

CsMT is a robust and streamlined CryoSPARC-based workflow to reconstruct undecorated and decorated microtubule maps. By unifying the processing pipeline into a single user-friendly tool, it makes high...

doi.org

A practical approach for ligand validation during structure determination is outlined and key pitfalls are illustrated by re-examining several deposited PDB entries #LigandValidation #XRayCrystallography #StructureGuidedDrugDiscovery doi.org/10.1107/S205...

Product-stabilized filamentation by human glutamine synthetase allosterically tunes metabolic activity pubmed.ncbi.nlm.nih.gov/42862727/ #cryoEM

New on the #cryoET Data Portal: three datasets benchmarking the crossed laser phase plate (xLPP) ⚡ Data by Reza Paraan and the @biohub.org team with collaborators at UC Berkeley. Full credits at the end of the 🧵 cryoetdataportal.czscience.com/depositions/10363

🔬 I’m hiring a postdoc to join my group at the Rosalind Franklin Institute! Contact me if you’re interested in using cryo-ET to study the molecular organisation of human brain tissue! 📍 Harwell, UK 📅 Deadline: 8 Nov opportunities.rfi.ac.uk/vacancy/rese...

Assembly and activation of the AAA+ motor of the multi-subunit cobaltochelatase www.biorxiv.org/content/10.64898/2026.10.09.757910v1 #cryoEM

In summary: - the graph that is circulating looks fishy - it is not clear what the source data for this graph is - the person who posted it first (?) is evasive. Could we (and especially politicians, journalists and scientists who should know better) please stop sharing unverified images?

Currently, a diagram is making the rounds that is claimed to show a significant reduction in Russian disinformation bot activity after Ukrainian drones hit Yandex data centers. There is no evidence that this plot shows real data. I suspect that this image is AI-generated.

Happy to share my PhD work on the regulatory mechanism of E3 ligase RNF213! RNF213 is not a monomer, but a dimer that switches between inhibited and activated states in an ATP-dependent manner. We tracked the activation mechanism and showed how disease mutations can destabilize the inhibited state.