Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Glad to see this out! Congrats @helenawatson.bsky.social and Victoria, and thanks @eisfabian.bsky.social for the support! Context is key in situ, and making the most out of your hard fought (inc. lifted out) lamella is important. Give BIGSMALL a go!

Helena Watson@helenawatson.bsky.social · 3mo ago

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

Excited to share our work on the structure and function of cytoplasmic lattices within mouse embryos. A collaborative effort with @niakanlab.bsky.social and work led by @kashishsingh.bsky.social and @inaharasimov.bsky.social . It is now out on BioRxiv: www.biorxiv.org/content/10.6...

The debate around VAR is really something: but let’s remember that the main reason why people advocated for VAR in football was to try to rectify those really mad decisions that that clearly spatially compromised linepersons got wrong. Everything since has just made it a farce. @bbcmotd.bsky.social

Very happy to say that our work on how axonemes are generated is now available on bioRxiv: www.biorxiv.org/content/10.6... - if you’ve ever wondered how the incredibly beautiful, complex structures within motile #cilia are constructed then this is for you!

80th Anniversary of Harwell Science Campus, and @diamondlightsource.bsky.social have powered up the UK’s most powerful laser for a few hours to celebrate. A pillar of light 8km high into the sky. A view from my front door.

We are thrilled to share our new pre-print on the molecular anatomy of nuclear proteasome in human sperm cells! #In-cellcryo-ET + #SPA + #LM! @piotrkolata.bsky.social @a-dsantos.bsky.social, @tomdendooven.bsky.social!! doi.org/10.64898/202...

An Asgard archaeon with internal membrane compartments Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors! www.biorxiv.org/content/10.1...

Our new preprint is out 🥳🥳🥳 Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence? 👇

Have you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? 🧊 Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts! 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-... 📍 Frankfurt | 📆 April 12–20, 2026

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

We have an open post-doc position in my group to study mRNA cleavage and polyadenylation using biochemical reconstitution and cryoEM. Please get in touch if you are interested in joining this amazing team! 🔬🧬🤩 #RNA #cryoEM

MRC Laboratory of Molecular Biology@mrclmb.ac.uk · 11mo ago

We’re looking for a #postdoc to join Lori Passmore’s group, within a programme aimed at understanding addition of mRNA poly(A) tails More info: www.nature.com/naturecareer... Closes 06 OCT #ScienceJobs #PostdocJobs

Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on! #TeamTomo #PlantScience 🧪 🧶🧬 🌾 elifesciences.org/articles/105... 1/🧵

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit www.nexperion.net/semcm2026

The Dunn School PhD programme is live! 🎉 Two projects in our labs: 1) How the motor protein giant dynein-2 works 2) How signalling-essential membrane proteins get into cilia Cryo-EM/ET, single mol & live-cell fluor microscopy, CRISPR-based genetic tools & more! www.path.ox.ac.uk/study-with-u...

🚨 We are hiring a #postdoc in Quantitative Cell Biology/Biochemistry. Join us if you are interested in using super-resolution STED & cryoET to uncover self-organization dynamics of epithelial cell interfaces & glycobiology collaboration with WuLab zurl.co/aTGq7

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social www.biorxiv.org/cgi/content/... @mrclmb.bsky.social @wellcometrust.bsky.social

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...! Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more! Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

The small GTPase Ran defines nuclear pore complex asymmetry

Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

sciencedirect.com

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...

rdcu.be

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo www.biorxiv.org/content/10.1...

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells

In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...

biorxiv.org

New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

And here it is now in print! www.cell.com/cell-reports... With a front cover splash 😍

Michael Grange@michaelgrange.bsky.social · last yr.

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

We’re hiring a postdoc! 🔬 Join us in Bristol, UK to study in situ structural immunology using cryoFIB-SEM + cryoET on our new Hydra Bio system. ERC-funded, 3 years, studying phagocytosis to develop new immunotherapeutics. Closing 29 July 2025. Info + apply: www.bristol.ac.uk/jobs/find/de...

🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...

A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue pubmed.ncbi.nlm.nih.gov/40527314/ #cryoEM

New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM tinyurl.com/257sw7j9 Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1

Repost appreciated: Open #PhDposition in structural virology in my group! Join us in Umeå, 🇸🇪 to uncover how arboviruses remodel the cellular interior. In situ #cryoET combined with #virology, #cellbiology, and #biophysics. Deadline 4 May. More info: www.carlsonlab.se/join/

Check out our paper on Xenon milling of HPF samples, now online @naturecomms.bsky.social rdcu.be/eczIb