Do you want to label the nuclear envelope? Do you work on a non-model species? Try Concanavalin A! We tested ConA on various species across the eukaryotic tree and find it is a very useful ExM-compatible marker for the NE and ER. Read all about it in our preprint: www.biorxiv.org/content/10.6...

Concanavalin A as a pan-eukaryotic nuclear envelope marker for expansion microscopy

Across eukaryotes, the nuclear envelope exhibits distinct remodelling strategies during mitosis: complete breakdown (open mitosis), partial disruption (intermediate), or full retention (closed). Howev...

biorxiv.org

I am looking for a lab manager for my group that I will be starting at the @fmiscience.bsky.social in February! We will use in situ structural biology to investigate dynamic ciliary processes. More info here —> bsky.app/profile/comp... #cilia #teamtomo #proteomics #protistsky

FMI science@fmiscience.bsky.social · 2w ago

📢 We’re hiring! Join Caitlyn McCafferty’s (@computingcaitie.bsky.social) new group as Lab Manager and help shape lab operations, develop protocols and support research on the structure and dynamics of motile cilia. Apply by October 9: www.fmi.ch/education-ca...

Lipids build the membranes of all cells, but identifying their precise functions is extremely challenging. Here’s our attempt to change that: How to find and understand lipid-protein interactions, by Katelyn Cook & company. www.biorxiv.org/content/10.6...

The artist formerly known as #thatmitoriboguy has now become #thatchlororiboguy Check out some cool tomo from @florentwaltz.bsky.social @phaips.vd.st

Florent Waltz@florentwaltz.bsky.social · 2mo ago

This story is now published in @natplants.nature.com ! 🎉🎩 Check out the final article here: nature.com/articles/s41477-026-02361-1 Very similar to the original bioRxiv preprint, with the addition of some cool polysome analysis (in the supplementals)! Thanks and congrats to everyone involved! 🙏

New Assistant (tenure-track) or Associate Professor position in Climate #Biophysics! Join our international research community to explore fundamental molecular mechanisms underlying climate-relevant biological processes. Apply by 7 Sept 2026! www.biozentrum.unibas.ch/de/open-posi...

Join Caitie to study ciliates and gain experience with integrative structural biology approaches! She's an amazing person and scientist, working together in the same lab has been so fun and I've learned so much from her Congrats miss 🎀

Caitie McCafferty@computingcaitie.bsky.social · 3mo ago

Happy to finally share that I will be starting my independent group at the @fmiscience.bsky.social in Feb 2027 in a vibrant new environment. We will use integrative in situ structural cell biology methods to investigate ciliary structure and dynamics in several model (and non-model 👀) systems!!

The 14th International Conference for Plant Mitochondrial Biology😍🍜🍀 My first trip to Japan and my first presentation award! Many thanks to the organizers, my supervisor, @florentwaltz.bsky.social, and our wonderful collaborators! I was happy to present my work in beautiful Kagoshima🌋

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

What if you could cut out sub cellular structures and measure the proteome? SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection www.biorxiv.org/content/10.6... awesome work by Curdin Franziscus, @imcf-lex.bsky.social, @biehli.bsky.social, Alexander Schmidt!

I'm excited to share *Stoic*, a method for fast and accurate protein complex stoichiometry prediction directly from sequence. Preprint: www.biorxiv.org/content/10.6... 🧵👇(1/10)

Excited to announce I’m starting a new lab at @gmivienna.bsky.social (Vienna BioCentre) in Sept 2026 We study how carbon fixation is inherited and maintained in algae using cryo-ET, imaging, environmental sampling&more Looking for people who want to build the lab together, get in touch! #teamtomo

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

For #ECR’s: DBG’s Hot Tpoic Workshop on Cryo-Electron Tomography + FIB-milling 13 – 15 April 2026 In @cellarchlab.com 's lab @biozentrum.unibas.ch Organised by @tamb-o.bsky.social, Karen Zinzius, @fannyleblanc.bsky.social Details: www.deutsche-botanische-gesellschaft.de/en/about-us/...

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

bioRxivpreprint@biorxivpreprint.bsky.social · 9mo ago

The vault associates with membranes in situ https://www.biorxiv.org/content/10.64898/2025.12.12.693869v1

We’re back with the next DinoSphere Online Seminar! Join us on Nov 4th, we're hosting: Edmée Royen (ULiège) - Symbiodinium
 Nicolas dos Santos Pacheco (Cambridge) - Perkinsids 📅 Tue, Nov 4 - 4 PM CET/3 PM GMT 
💻 Zoom link: sites.google.com/view/dinosphere
 Please spread the word! #protistonsky

That’s a wrap of the 2nd edition of our one of a kind structural biology block course at the @biozentrum.unibas.ch! In just 6 short weeks students designed protein oligomers; rotated in cryo-ET, cryo-EM SPA, AFM, and NMR labs; and expressed, purified, and structurally characterized their designs

How can we see the cells that make up a living organism? Membrane-localising tags can drive fluorescent proteins to the cell's outer membrane, making their outlines visible. But the tags don't work well in all organisms. How do you find one for your species of interest? 🧵 Check our latest preprint

A toolkit for testing membrane-localising tags across species

Transgenic markers and tools have revolutionised how we study cells and developing organisms. Some of the elements needed to construct those tools are universally applicable (e.g. fluorescent proteins...

biorxiv.org

Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social! Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬. Huge thanks to the DCI for making the event possible! See you next year! 👋

Pretty excited to share our new preprint! Non-photosynthetic Plastid Replacement by a Primary Plastid in the Making www.biorxiv.org/content/10.1...

Nudibranchs as pictured by a Japanese illustrator named Kumataro Ito, artist for the USS Albatross’ Philippine Expedition, 1907–10. More of his stunning images here: publicdomainreview.org/collection/k...

🌱 Using ‘compelling’ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture. buff.ly/j3TSIkn

New paper is out! We have studied the cute tiny Discocelia plataet sp. n. to obtain the first molecular data of the order Discocelida and clarify its cell structure. All this for a better understanding of the evolution of Cercozoa. onlinelibrary.wiley.com/doi/epdf/10....

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism. www.nature.com/articles/s41...

Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work. www.nature.com/articles/s41...

Structural insights into transcriptional regulation by the helicase RECQL5 - Nature Structural & Molecular Biology

Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s ...

nature.com

We’re kicking off the DinoSphere Online Seminar Series! Join us for our first session with Karel Mockaer (Heidelberg) & Yong Heng Phua (OIST) 📅 1 July 9AM CEST 🔗 tinyurl.com/4mjaverj Spread the word! @protistwtmostest.bsky.social @ehehenberger.bsky.social @chandnibhickta.bsky.social&Norico Yamada

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨

Science Magazine@science.org · 2y ago

In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion. Learn more in this week's issue: scim.ag/3FA3Ygq

We (@cellarchlab.com peeps, @manondemulder.bsky.social @phaips.vd.st @fannyleblanc.bsky.social @computingcaitie.bsky.social and Ricardo) had a blast at the EMBO in situ meeting in Heidelberg! Meeting old friends and making new ones! Lots of cool science and new ideas to try 👨🏻‍🔬🔬

This one was delayed for a while… apologies to the musicians. Collab with Matt, Will and Lorna (over on Twitter). And a great dev effort from Lorenz @lorenzlamm.bsky.social. Ofc @cellarchlab.com team. Comment if you want to help. #teamthylakoid www.biorxiv.org/content/10.1...

Molecular architecture of thylakoid membranes within intact spinach chloroplasts

Thylakoid membranes coordinate the light reactions of photosynthesis across multiple scales, coupling the architecture of an elaborate membrane network to the spatial organization of individual protei...

biorxiv.org

Feeling super grateful to have participated in the #EMBOStructBio workshop in Hamburg last week! The talks and tutorials were top-notch and it was great fun connecting with everyone :)