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Hannah Long

@hannahlong.bsky.social

Group Leader @mrc_hgu investigating gene regulation in development & human disease

71 Posts2036 Follower1336 Folgt
Naomi Moris@nmoris.bsky.social · 4mo ago

Following several years (!) of discussion and rumination, our team of excellent colleagues - Martin Pera, @nicolasrivron.bsky.social, @amartinezarias.bsky.social, Karen Sermon and Nienke de Graeff - have published a new ethical perspective on SCBEM #embryomodels rdcu.be/fcklG [1/5]

Morally relevant features warranting ethical oversight in human stem cell-based embryo models

Nature Cell Biology - This Perspective presents a set of attributes by which to evaluate human stem cell-based embryo models that not only include morally relevant embryo features but also take...

rdcu.be

MRC Weatherall Institute of Molecular Medicine@imm.ox.ac.uk · 5mo ago

#WIMMReads 🧪 From Jim Hughes, Douglas Higgs and Mira Kassouf: actively transcribed genes can define chromatin boundaries, linking gene activity directly to 3D genome organisation. Read more 👇 #GenomeBiology #Chromatin #WIMM @rdm.ox.ac.uk @medsci.ox.ac.uk #AkademicSky

A functional overlap between actively transcribed genes and chromatin insulator elements - The EMBO Journal

The mammalian genome is organised into large topologically associating domains (TADs) and smaller sub-TADs or enhancer-promoter loops, which may contribute to the regulation of gene expression. These ...

doi.org

Sahin Naqvi@sahinnaqvi.bsky.social · 6mo ago

We are looking for two exceptional Research Assistants! 1) Experimental, to drive forward quantitative studies of transcription factor function in development 2) computational, to develop and apply cutting-edge deep learning models of gene regulation. Links below

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Ewa@ewaoz.bsky.social · 7mo ago

📣 Preprint alert! I am happy to share that our neural crest manuscript is now available on BioRxiv! www.biorxiv.org/content/10.6...

Array-CNCC: precise aggregation and arrayed plating facilitate quantitative phenotyping of human cranial neural crest cells and craniofacial disease modelling

Facial development is highly sensitive to genetic and environmental perturbation, with craniofacial malformation associated with over one-third of congenital birth defects. The face arises during an early and largely inaccessible window of embryonic development, with a large contribution from transient and multipotent cranial neural crest cells (CNCCs). Assessment of the molecular and cellular mechanisms driving normal and disordered human facial development therefore relies greatly on the use of in vitro cellular models. Here, we adapted a neurosphere-based CNCC differentiation protocol to facilitate robust quantification of early specification and migration events. Introduction of single-cell aggregation with arrayed plating enabled standardisation of neurosphere size, growth and patterning. Inclusion of fibronectin coating enhanced the efficiency of neurosphere attachment and synchronicity of CNCC migration timing. To demonstrate application of the Array-CNCC method, we developed a strategy for mosaic co-culture, which can facilitate differentiation of wildtype untreated cells directly alongside cells exposed to distinct drug treatments or genetic alterations. Finally, we present a screening approach which we use to test the impact of distinct extracellular matrix components on neurosphere morphology, CNCC migration and gene expression. Together, the Array-CNCC method is highly amenable to quantitative phenotyping and screening approaches, enabling enhanced craniofacial disease modelling with both cellular and molecular readouts. ### Competing Interest Statement The authors have declared no competing interest. Medical Research Council, MC\_UU\_00035/12, MC\_ST\_00035 Wellcome Trust, https://ror.org/029chgv08, 227712/Z/23/Z

biorxiv.org

Eric Conway@conwayer1.bsky.social · 7mo ago

Super line-up of chromatin, epigenetics and gene regulation speakers coming your way from @aicc-ireland.bsky.social over the next 5 months!

All-Ireland Chromatin Consortium@aicc-ireland.bsky.social · 7mo ago

After a short break in our webinars we are delighted to be back up & running this month! First up, on Jan 30th we will be joined by @lucas.farnunglab.com from @harvardmed.bsky.social 🔬 Shortly after, on Feb 13th we will be hosting @heardlab.bsky.social from @crick.ac.uk 🧬 Registration details 👇

Alejandro Brenes@ajbrenes.com · 8mo ago

Excited to share our work at @natcomms.nature.com We used single cell proteomics to define the functional heterogeneity of human neutrophils in glioblastoma, finding pro and anti-tumorigenic effector states invisible to scRNAseq. SCP will revolutionise immunology, this is just the start

Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma - Nature Communications

Neutrophils infiltrate glioblastomas with the capacity to engage pro/anti tumoural responses. Here the authors developed proteomic workflows to stratify neutrophil heterogeneity by function. This work...

nature.com

Alejandro Brenes@ajbrenes.com · last yr.

Happy to share our latest preprint doing low cell number (mini-bulk) and single cell #proteomics on tumour associated neutrophils from human glioblastoma where we find multiple functional states that would be invisible to scRNAseq, some showing pro-tumoural states with potential therapeutic value

Rob Klose@robklose.bsky.social · 8mo ago

An early Christmas present for those interested in chromatin and transcription! Fantastic work from @au-ho-yu.bsky.social and @aleksszczurek.bsky.social . Thanks to Inge and Michiel for their help. Please repost! www.biorxiv.org/content/10.6...

SET1/MLL complexes control transcription independently of H3K4me3

Histone H3 lysine 4 trimethylation (H3K4me3) at gene promoters is thought to play a central role in gene transcription. H3K4 methylation is deposited by the SET1 (A/B) and MLL (1-4) multi-protein comp...

biorxiv.org

Damir Baranasic@da-bar.bsky.social · 8mo ago

JASPAR 2026 is out 🎉 The new release massively expands the TF motif collections and adds a dedicated DeepLearning collection of motifs learned from deep learning models. Database: jaspar.elixir.no Paper (NAR): doi.org/10.1093/nar/... 🧵1/2

JASPAR: An open-access database of transcription factor binding profiles

JASPAR is the largest open-access database of curated and non-redundant transcription factor (TF) binding profiles from six different taxonomic groups.

jaspar.elixir.no

Filipa Simões@simoesfilipa.bsky.social · 8mo ago

Don’t miss out on this opportunity- come and join us at the Oxford Organoid Hub working under the MRC-BHF REACT programme! If you love in vitro 3D models, the ❤️ and regenerative therapies, this is for you - apply by 18 Dec @oxforddpag.bsky.social @idrm.ox.ac.uk @rdm.ox.ac.uk

Chris Toepfer@christoepfer.bsky.social · 8mo ago

Some good news, myself and @simoesfilipa.bsky.social are advertising for a post-doctoral researcher to join us at the Oxford Organoid hub: tinyurl.com/OOHJob Come and join us to develop novel human organoid systems as part of the MRC/BHF CRE in advanced cardiac therapies.

Stefan Barakat@stefanbarakat.bsky.social · 9mo ago

Very pleased to share our latest paper published in Cell: BRAIN-MAGNET: A functional genomics atlas for interpretation of non-coding variants: Cell www.cell.com/cell/fulltex... @cellpress.bsky.social, @cp-cell.bsky.social, @ruizhideng.bsky.social #enhancer here is a thread about our findings:

BRAIN-MAGNET: A functional genomics atlas for interpretation of non-coding variants

BRAIN-MAGNET, a convolutional neural network trained on 148,198 functionally tested non-coding regulatory elements, predicts enhancer activity directly from DNA sequence and identifies nucleotides ess...

cell.com

Hannah Josefine Jüllig@hannahjuellig.bsky.social · 9mo ago

Excited to share our new paper out today in Development! Check out Hannah’s fantastic post below for a summary of the findings ✨ journals.biologists.com/dev/article/...

Hannah Long@hannahlong.bsky.social · 9mo ago

📣 Paper alert! I am delighted that our paper exploring the impact of Neanderthal-derived variants on the activity of a disease-associated craniofacial enhancer has been published in Development today! journals.biologists.com/dev/article/...