A Sticky Dance Between a Cell and a Neuron. #neuroscience #science #mysterious #HiddenWorld #cell #FluorescenceFriday ➡️A Schwann cell precursor interacting with sensory axons 🔬🧫🧪
Alexandre Dumoulin
@axonsalex.bsky.social
Developmental Neurobiologist at UZH | Microscopy Enthusiast. Using the chicken embryo to decipher the molecular mechanisms of neural circuit formation. Fascinated by axonal growth cones, primary cilia, and extracellular vesicles.
This Is What Drives Axon Growth. Axon growth is powered by the actin cytoskeleton at the growth cone. Here, filamentous actin dynamics drive fast, directed axon extension in real time. #Fluorescencefriday #science #neuroscience #microscopy 🧪
Proximity labeling users: check out this study by @zhixingchen2.bsky.social and Peng Zou, which establishes how much contact-dependence vs. diffusion is responsible for biotinylation — tl;dr TurboID has a much smaller effective radius! www.nature.com/articles/s41... 1/2
Spatial barcoding reveals reaction radii and contact-dependent mechanism of proximity labeling - Nature Chemical Biology
A DNA nanoruler platform enables precise measurement of the labeling radii of TurboID and APEX2, uncovering their contact-dependent mechanisms. These findings redefine the enzymology of proximity labe...
nature.com
Happy #FluorescenceFriday! Temporal projection of a time-lapse recording decided to become abstract art. Pretty splashy, isn't it? PalmBow-labeled commissural axons in culture. #neuroscience #microscopy #livecellimaging 🧪
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky
Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪
Spirochrome@spirochrome.com · 10mo agoHuge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition! His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin. www.nikonsmallworld.com/galleries/20... #FluorescenceFriday
Check it out at PNAS today. Thank you Alexandre for the collaboration!! PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by Jing Ling, Yitong Liu, and all the collaborators! Available @spirochrome.com
Alexandre Dumoulin@axonsalex.bsky.social · last yr.Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social ! We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience tiny.cc/3qhp001
Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social ! We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience tiny.cc/3qhp001
Hot off the press: The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience: rdcu.be/ek010
Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster
Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...
rdcu.be
New from the lab: @maheandria.bsky.social and colleagues in collaboration with the Hiesinger, Stoeckli and von Kleist labs dissect the sequential emergence of axonal targeting during development in today's issue of Nature Neuroscience: rdcu.be/ek010
Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster
Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...
rdcu.be
These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.
it is a filopodial (thin protusions) party in there! 🕺🧐 🧪 #liveimaging
Alexandre Dumoulin@axonsalex.bsky.social · last yr.This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging. I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉 🧪
This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging. I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉 🧪
Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!
A brief history of The Company of Biologists - 100 years of supporting scientists!👍👏 journals.biologists.com/jcs/article/...
The Company of Biologists: celebrating 100 years
Summary: As we begin celebrating our 100-year anniversary, we reflect on our extraordinary story so far, consider the Company's current support for our communities and prepare for some inspiring activ...
journals.biologists.com
Join us in beautiful Quintay to celebrate 25 years of the Practical Course on Developmental Biology! To register go to biodesarrollo.unab.cl/mini-symposi... Come to enjoy a unique blend of science, sea, and food – all for free!
Did you know 2024 was our 20th anniversary? We're closing it out by sharing a brief history of Addgene, with commentary from our incredible community. Read it below! blog.addgene.org/add...
Addgene: The First Twenty Years
Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.
blog.addgene.org
2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)
How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling. www.biorxiv.org/content/10.1... Special thanks to @aliceyting.bsky.social for discussions
I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social.
For this #FluorescenceFriday, let's watch an axonal growth cone (for a change) displaying its most beautiful filopodia (this time)! 🤩 They are so dynamic! 1 image taken every 500 ms for 5 min played at 15 fps. 🧪
I used VVD Viewer for this #FluorescenceFriday video: 141 volumes from the #HHMIJanelia FlyLight Split-GAL4 #Drosophila driver collection. The animation comes from a high-level description processed by neuVid. Such a rapid tour of the data has questionable scientific value, but it's fun to watch!
Lynn Landmesser was a giant in the field of developmental neurobiology. Sad to hear of her passing. #DevBio #neuroscience 🧪
Britton Sauerbrei@brittonsauerbrei.bsky.social · 2y agoI am saddened by the passing of Lynn Landmesser, a pioneer in neural development and cornerstone of our department. When I arrived, she was still teaching students to fabricate electrodes and dazzling junior faculty with stories about the history of the field. She will be missed.
This axonal growth cone from a culture of spinal neurons decided to display its most beautiful lamellipodia for this #FluorescenceFriday! 🤩 #neuroscience #liveimaging 🧪 1 image taken every second for 10 min, played at 15 images/s
Excellent preprint from @goodfrognosis.bsky.social’s lab, showing convergence of #autism related proteins at the #cilium (sure to interest people working on cilia/centrosome on this platform; ping @cilialab.bsky.social) www.biorxiv.org/content/10.1...
Convergence of autism proteins at the cilium
Hundreds of high confidence autism genes have been identified, yet the relevant etiological mechanisms remain unclear. Gene ontology analyses have repeatedly identified enrichment of proteins with ann...
biorxiv.org
Come to be our colleague @pasteur.fr! center of Paris, great colleagues, outstanding facilities, permanent position, crème brûlée every Friday - what else could one want?
Nadia Izadi-Pruneyre@nadia-izadi.bsky.social · 2y ago📣📣Group leader positions @pasteur.fr 👇
Viral tracing (yellow) in the mouse adult spinal cord (cyan, nuclei; magenta, axons). Image by the very talented Soizic Riche. #fluorescencefriday #fluorescentfriday
It’s time for another #FluorescenceFriday! 👩🎨🎨🔬🫀multi-color labeled zebrafish heart with mosaic expression of diverse myl7:color plasmids 🏳️🌈🌈
