Thrilled to share a new important study from the lab, published today in @nature.com, showing how cohesin mediates efficient replication fork plasticity and stress response, using its loop extrusion activity to promote 3D contacts on replicating DNA: www.nature.com/articles/s41...

Cohesin reshapes replication fork contacts to aid fork slowing and reversal - Nature

Cohesin-mediated loop extrusion limits sister-fork coupling and tethers nearby replication forks under replication stress, promoting fork reversal and slowing fork progression to safeguard genome...

nature.com

Ten years ago, our director, Stefan Jentsch, passed away. A memorial symposium will be held in his honour on October 23. Many of his former colleagues and friends will be in attendance. Would you like to join? Please register until October 1: ❕https://eveeno.com/256035281 #Ubiquitin #Symposium

🧵 We’re excited to share our new bioRxiv preprint on how INO80 reads promoter-proximal DNA sequence features to position +1 nucleosomes 🧬 Amazing work by Mariia, Annika, and others! 🤩 🔗 www.biorxiv.org/content/10.6... #ChromatinBiology #StructuralBiology #Nucleosome #INO80

‼️🚨Out now in Molecular Cell - Our latest study 𝐫𝐞𝐯𝐞𝐚𝐥𝐢𝐧𝐠 𝐧𝐞𝐰 𝐢𝐧𝐬𝐢𝐠𝐡𝐭𝐬 𝐢𝐧𝐭𝐨 𝐅𝐀𝐂𝐓 𝐟𝐮𝐧𝐜𝐭𝐢𝐨𝐧 𝐚𝐧𝐝 𝐭𝐡𝐞 𝐢𝐦𝐩𝐨𝐫𝐭𝐚𝐧𝐜𝐞 𝐨𝐟 𝐧𝐮𝐜𝐥𝐞𝐨𝐬𝐨𝐦𝐞 𝐨𝐫𝐠𝐚𝐧𝐢𝐬𝐚𝐭𝐢𝐨𝐧 𝐟𝐨𝐫 𝟑𝐝 𝐠𝐞𝐧𝐨𝐦𝐞 𝐦𝐚𝐢𝐧𝐭𝐞𝐧𝐚𝐧𝐜𝐞: tinyurl.com/2xctvx38. It was a pleasure to lead this collaborative effort alongside @nilskrietenstein.bsky.social More in🧵below 🪡

Maintenance of nucleosome organization through replication and transcription counteracts aberrant coalescence of active chromatin

Nucleosomes with their associated modifications organize and regulate the genome. It is unclear how this is integrated with the requirement of replica…

tinyurl.com

Delighted to share our latest publication, a collaborative work with @hanasedlackova.bsky.social and @puckknipscheer.bsky.social. Grateful to everyone who contributed to this project. www.cell.com/cell-reports...

FANCJ and RTEL1 facilitate pre-replication complex disassembly following replisome collision

Cameron et al. show that dormant pre-replication complexes are removed upon collision with replication forks in Xenopus egg extracts. They identify FANCJ and RTEL1 as key factors in this process and s...

cell.com

Finally out! I am super proud of this work that took 6 years to come to fruition, with many amazing people involved, in particular Sadegh, Ibrahim, Jelmer. #SadeghFeiz: all kudos for you to push this project through! Great Collab with Anssi Malinen from @utu.fi @vuamsterdam.bsky.social

bioRxiv Biophysics@biorxiv-biophys.bsky.social · last mo.

A multimodal, correlative magnetic tweezers-TIRF platform for high-throughput single-molecule interrogations https://www.biorxiv.org/content/10.64898/2026.08.11.744120v1

Happy to share a new review article from our group: “Timing the origin: chromatin, transcription, and the spatiotemporal control of eukaryotic DNA replication”. I’m proud to see how the work of several students in my group has come together in this article. @imbmainz.bsky.social @sfb1361.bsky.social

After nearly 7 years, it’s time to move on from the LMB 😭 Huge thanks to Joe and all the fantastic members of the lab, as well as all the talented people I was lucky enough to work with. Really excited to be starting my own group at the Department of Biochemistry in Oxford. Wish me luck 🤞

🧬 Delighted to share our new Nature Communications paper! We show how MCM2-7 double hexamers become active helicases and trigger DNA melting in vivo. Led by Chris Weekes & L. Maximilian Reuter. Congratulations to the whole team! doi.org/10.1038/s414... #DNAReplication

Preprint alert! Using smFRET, we define the only essential ATP hydrolysis step in translation initiation. We show that eIF3 and eIF4B stimulate ATP-hydrolysis-dependent recycling of cap-stalled eIF4F, coupling eIF4F recycling to PIC loading onto an mRNA.🔬 doi.org/10.64898/202...

The replication-risk sequence is an essential bacterial DNA sequence linked to DNA gap formation. For the first time, we directly observed this gap formation at single-molecule resolution! We show that the gaps occur exclusively on the lagging strand and reveal why. www.biorxiv.org/cgi/content/...

Copy-back RNA synthesis by coronavirus polymerase requires helicase activity and is stimulated by remdesivir and molnupiravir | Science Advances www.science.org/doi/10.1126/... #Coronavirus #SARS2 #COVID19 #singlemolecule #biophysics #magnetictweezers

Copy-back RNA synthesis by coronavirus polymerase requires helicase activity and is stimulated by remdesivir and molnupiravir

Helicase-driven copy-back RNA synthesis in coronaviruses reveals how antivirals trap replication intermediates.

science.org

If you have experience in biochemistry/cryo-EM, are interested in DNA replication, and would like to do a postdoc in New York - our lab has positions available! Just DM or email me with your CV.

Out now in Science! Our study challenges long-standing assumptions about transcription factor specificity in eukaryotes. Novel single-molecule measurements of TF behavior in living cells reveal an independence of locus-specific binding from DNA sequence recognition.🧵 www.science.org/doi/10.1126/...

Unstructured transcription factor interactions enable emergent specificity

How intrinsically disordered regions (IDRs) shape chromatin binding and nuclear organization of transcription factors (TFs) remains unclear. We used proximity-assisted photoactivation (PAPA), a single...

science.org

Very happy to announce that Tom Perkins and I will be organizing the "Physics of Life at the Nanoscale" meeting at the Aspen Center for Physics Jan 3-8, 2027. Please save the date!

Join us in Nottingham in September for the UK DNA replication meeting 🧬. Super EarlyBird deadline today and abstract deadline in 1 month. www.eventsforce.net/biochemsoc/f...

⚡New preprint from the lab⚡: A key early checkpoint in gene expression is promoter proximal pausing of RNA polymerase II. For over 20 years, we as field have not been able to recreate pausing under realistic cellular conditions. @robertovn.bsky.social shows that ELOF1 is the missing piece.

Roberto Vázquez Núñez@robertovn.bsky.social · 4mo ago

🚨 New preprint: GATO-seq did it again! ELOF1 is the missing promoter-proximal factor that confers RNA Pol II resistance to TFIIF. Including ELOF1, DSIF, and NELF in GATO-seq reactions recapitulates promoter-proximal pausing in vitro at physiological conditions for the 1st time. tinyurl.com/ELOF1

New preprint from our lab! We've been working out how the CMG replicative helicase gets built. 🧵 1/3

Out today - structure of the human HIRA histone chaperone complex bound to nucleosomes. Ever wondered how nucleosomes are assembled in the wake of transcription? It takes a 'hulk of a protein complex'. Work by the amazing Wei Tian weetian558.bsky.social. www.biorxiv.org/content/10.6... 🧵

Small contribution to the eternal match happening inside every cell: in the red corner, replication. In the blue corner, transcription. Both convinced they own the genome. Another amazing collaboration with @hamperllab.bsky.social and @luijsterburglab.bsky.social lnkd.in/ey9tH7YF

Structure of the Pre-Initiation Complex Explains CMGE Biogenesis https://www.biorxiv.org/content/10.64898/2026.05.15.725318v1

Mcm10 braces converging replicative helicases to pull apart origin DNA https://www.biorxiv.org/content/10.64898/2026.05.15.725099v1

We're hiring! Join our growing team at the @crg.eu as a postdoc. Curious about how mRNA translation and decay cross-talk? We're looking for a postdoctoral researcher to lead a project in the field of RNA biology and gene regulation. Apply here: recruitment.crg.eu/content/jobs... Please share!

Save the date! 🗓️ On October 3, we invite you to the #OpenHouse at the #Garching research campus. ⚗️🧬 Experience research up close with talks, hands-on activities, tours & more: www.tum.de/en/open-day #OpenCampus #HigherEducation 📹Forschungscampus Garching

“I think it’s a real mistake to forget where the pipeline for all this begins.” John Diffley reflects on the importance of basic research, his mission to decode DNA replication and recreate it in the lab, and the scientific optimism of the 1960s that helped shape it. www.crick.ac.uk/news/2026-03...

Celebrating the 1st anniversary of the Höpfler lab! 🎂 🥂 The cactus-ribosome translating this special message now decorating my office... Thanks Stefania, Laia & Mariana for the creative surprise!

In DNA-PAINT, nearby proteins create overlapping localizations. You may see two by eye, but analysis often misses them, wasting precious precision. G5M solves this. Now in @natcomms.nature.com. doi.org/10.1038/s414.... 1/7

Molecular mapping in DNA-PAINT via modified Gaussian Mixture Modeling - Nature Communications

Standard algorithms fail to fully exploit the spatial information in DNA-PAINT. Here, the authors present G5M, an algorithm providing molecular maps by accurately inferring biomolecule positions at di...

doi.org

Fantastic news! 🙌 The Perutz is excited to welcome Danny Nedialkova as a new group leader starting this April! 🎉 She will strengthen the institute's research on the fundamental mechanisms that shape cellular physiology and disease ➡️ www.maxperutzlabs.ac.at/research/res...

Get to know Thomas Schlichthärle, our newly appointed professor of #AI-guided #ProteinDesign, in the latest NewIn episode. He wants to use custom-designed #proteins to advance the development of new #medicines: go.tum.de/484027 #HightechAgendaBayern ▶️ youtu.be/G-pt28HJpA8 📹& 📷ProLehre

Designing proteins for targeted cell activation

Prof. Thomas Schlichthärle spricht über seine Arbeit als Professor für AI-guided Protein Design.

go.tum.de

Please join Andreas Ladurner and me for the GRC Chromatin Structure and Function conference to be held near Barcelona Spain from May 31-June 5! We accepting abstracts for posters and talks. The meeting is preceded by an awesome GRS. Learn more in our video below and apply www.grc.org/chromatin-st...