This paper represents a massive undertaking (6+ years!) led by then PhD student, now Postdoc Keying @keyinglia.bsky.social in collaboration with the Bob Harris lab and many amazing contributors. Thanks to everyone involved! 🧵3/3
Ethan Hughes
@ethanhugheslab.bsky.social
neuron-glial interactions, oligodendrocyte lineage, myelin, multi-photon microscopy @CUMedicalSchool https://www.ethanhugheslab.com/
🚀 Our new paper is out in Nature Communications! We uncovered a mechanistic switch to boost myelination: deleting Gtf2i in myelinating glia enhances Sox10/Mbp activity → thicker myelin, faster conduction, improved motor coordination. Open access link: www.nature.com/articles/s41...
Gtf2i-encoded transcription factor Tfii-i regulates myelination via Sox10 and Mbp regulatory elements - Nature Communications
The transcriptional regulation of oligodendrocytes has an essential role in myelin formation and maintenance. Here, the authors identify the transcription factor Tfii-i as a regulator of myelin genes expression in the nervous system and show that its loss enhances myelin thickness and nerve conduction.
nature.com
Thrilled to share our new paper @cp-cell.bsky.social: “Microglia–astrocyte crosstalk regulates synapse remodeling via Wnt signaling.” This work demonstrates the necessity of glia–glia communication and coordination during synapse remodeling. 🔗 www.cell.com/cell/fulltex... Faust et al., Cell 2025
If you're interested in trafficking of #myelin constituents in #oligodendrocytes, and of course you are, you may want to check out our new preprint. It's very nice PhD work by @sophiesiems.bsky.social, and we're grateful to all who contributed! www.biorxiv.org/content/10.1...
Adaptor protein complex 2 (AP2) participates in biogenesis and homeostasis of myelin sheaths in the central nervous system
Myelination of CNS axons requires oligodendrocytes to undergo extensive morphological changes by producing large amounts of myelin membrane with defined protein composition and structure. The formatio...
biorxiv.org
Our new paper on metformin, myelin, mitochondria and metabolism is now out at rdcu.be/eDbFD Thanks to Nina and all of the team!
Metformin alters mitochondria-related metabolism and enhances human oligodendrocyte function
Nature Communications - Metformin is under clinical trial for brain neuroprotection but how it may work is poorly understood. Here, the authors show that metformin helps myelin formation by human...
rdcu.be
www.nature.com/articles/s41...
FBXW7 regulates MYRF levels to control myelin capacity and homeostasis in the adult central nervous system - Nature Communications
The homeostasis of myelin in the central nervous system is highly regulated, but the underlying mechanisms are unclear. Here, the authors show that the FBXW7 protein has a role in constraining myelin ...
nature.com
Please RT: My lab in the Neuroscience Dept at MUSC is hiring a technician. We study oligodendrocytes — unique non-neuronal brain cells — using cutting-edge imaging. Recent grads especially encouraged to apply. Feel free to reach out! #neurojobs musc.wd1.myworkdayjobs.com/MUSC/job/Cha...
UNIV - Research Specialist I - Department of Neurosciences
Job Description Summary The Li Lab at MUSC is seeking a highly motivated and detail-oriented Research Specialist I to join our dynamic neuroscience research team. This position offers an excellent opp...
musc.wd1.myworkdayjobs.com
Discovery of oligodendrocyte enhancers that regulate Sox10 expression journals.plos.org/plosgenetics... #oligodendrocyte #myelin
Discovery of oligodendrocyte enhancers that regulate Sox10 expression
Author summary Oligodendrocytes are a special cell type in brain and spinal cord and form myelin sheaths around axons. Myelin sheaths are lipid-rich structures that are essential for the rapid transmi...
journals.plos.org
Fantastic new study on the role of TMEM63a in oligodendrocyte-dependent myelination in the CNS from the Swetha Murthy lab: pubmed.ncbi.nlm.nih.gov/40694323/
TMEM63A, associated with hypomyelinating leukodystrophies, is an evolutionarily conserved regulator of myelination - PubMed
Infantile hypomyelinating leukodystrophy 19 (HLD19) is a rare genetic disorder where patients exhibit reduced myelin in central nervous system (CNS) white matter tracts and present with varied neurolo...
pubmed.ncbi.nlm.nih.gov
We had fun assembling images and describing approaches for imaging oligodendrocytes and myelin in vivo, in organotypic slices, and in fixed tissues. This work started in the lab of @jgrutzendler.bsky.social over 10 years ago and continues to keep us busy. www.nature.com/articles/s44...
A nice Review on 3P neuroimaging in NR Neuro. The images never fail to amaze me. www.nature.com/articles/s41...
Three-photon microscopy: an emerging technique for deep intravital brain imaging - Nature Reviews Neuroscience
Optical microscopy allows neural cells to be studied in the intact brain, but imaging deep neural tissue presents substantial challenges. Prevedel and colleagues outline the principles of three-photon...
nature.com
Excited to share this new paper from the lab, now out in @cp-immunity.bsky.social! Led by the talented @bendevlin.bsky.social and in collab with @anneschaefer.bsky.social. a brief 🧵
Excitatory-neuron-derived interleukin-34 supports cortical developmental microglia function
Neuron-microglia interactions dictate the development of neuronal circuits in the brain. However, the factors that regulate these processes across dev…
sciencedirect.com
Excited to share that two studies from my postdoc research were published back to back! In the first, we uncovered the molecular basis of axon spheroids—a key hallmark of Alzheimer's disease—and demonstrated their potential reversibility. www.nature.com/articles/s43...
Subcellular proteomics and iPSC modeling uncover reversible mechanisms of axonal pathology in Alzheimer’s disease - Nature Aging
Axonal spheroids disrupt neural circuits in Alzheimer’s disease. In this study, using subcellular proximity labeling proteomics in human brain and iPSC modeling, the authors link spheroid formation to...
nature.com
Happy to share our latest publication on subcellular myelin proteomics and structural changes in Alzheimer disease www.nature.com/articles/s41...
It is out! Read how you achieve 1.7 mm deep 3-photon imaging in prefrontal cortex with dendritic spine resolution over a week. www.nature.com/articles/s42...
Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution - Communications Biology
Three-photon in vivo imaging provides access to medial prefrontal cortex, hippocampus and spinal cord in mice at up to 1.7 mm depth of neurons, dendrites, spines, astrocytes and microglia.
nature.com
For those who want a distraction and/ or are interested, here's the latest from the group, led by Phil Braaker www.nature.com/articles/s41...
Activity-driven myelin sheath growth is mediated by mGluR5 - Nature Neuroscience
Employing pharmacology, genetics and all-optical approaches in zebrafish, Braaker et al. find that neuronal activity influences the growth of myelin sheaths along axons by signaling through metabotrop...
nature.com
Our lab's 1st paper, led by @fchrisbennett.bsky.social & @waisenberg.bsky.social. A decade thinking how to directly assess the impact of microglia replacement. Yrs of hard work. This kind of work is needed to expand brain disease care for kids & the vision for safer, more effective cell therapy.
Immunity@cp-immunity.bsky.social · last yr.Online now: Direct microglia replacement reveals pathologic and therapeutic contributions of brain macrophages to a monogenic neurological disease
Excited to share this new work led by the talented Mike Dolan @dolan in collaboration with Evan macosko See below for details and preprint
Mike Dolan@dolanmjg.bsky.social · last yr.How does the brain repair myelin after injury? We mapped the full cellular choreography of white matter repair using snRNA-seq + Slide-seqv2, uncovering a type I interferon-driven glial program that recruits lymphocytes to promote remyelination. www.biorxiv.org/content/10.1... Details below 👇
Fun to have contributed a little to this, led by the team of the incomparable Guoqiang Yu. Suspect that this will prove to be an immensely popular image analysis platform for interrogation of dynamic signallingl! www.sciencedirect.com/science/arti...
Fast, accurate, and versatile data analysis platform for the quantification of molecular spatiotemporal signals
Optical recording of intricate molecular dynamics is becoming an indispensable technique for biological studies, accelerated by the development of new…
sciencedirect.com
Happy to see my work published in Journal of Neuroinflammation! Thanks to my amazing mentor @jkhuang.bsky.social and all the co-authors George Melchor, Meghan Bullard, @philgrossphd.bsky.social for their continued help and support. jneuroinflammation.biomedcentral.com/articles/10....
Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelination - Journal of Neuroinflammation
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) characterized by immune cell-driven demyelination and progressive neurodegeneration. Senescent cells (SCs)...
jneuroinflammation.biomedcentral.com
Excited to share our latest paper! We argue that in MS, the primary immune attack isn’t on myelin or myelinated axons — but on the ion & water homeostasis machinery in astrocyte endfeet. A short thread on this new perspective 1/11
Nature Reviews Neurology@natrevneurol.nature.com · last yr.New online! Multiple sclerosis: an immune attack on astrocyte-mediated ion and water homeostasis
Interested in #oligodendrocyte metabolism and/or #multiplesclerosis? Our paper is now at Biorxiv! We show that oligos express MCT2, the high affinity monocarboxylate transporter, well known for supplying neurons with astrocyte-derived lactate (astro-neuron lactate shuttle)👇
bioRxiv Neuroscience@biorxiv-neursci.bsky.social · 2y agoMonocarboxylate transporter 2 is required for the maintenance of myelin and axonal integrity by oligodendrocytes https://www.biorxiv.org/content/10.1101/2025.01.10.632306v1
🥼 Janelia researchers led by @boazmohar.bsky.social @nspruston.bsky.social @rhodamine110.bsky.social & @svoboda314.bsky.social @alleninstitute.bsky.social have developed a new imaging tool that maps brain-wide changes in neuronal connections. 🧠 🔗 www.janelia.org/news/new-ima...
journals.biologists.com/dev/article/... New work from the lab characterizing sensory-dependent transcriptomes in mouse V1 across neuronal and glial cell types. Some fun nuggets in here, and all data publicly available for download and re-analysis. Happy gene hunting!
A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex
Summary: Single-cell RNA sequencing of developing mouse visual cortex uncovers cell type-specific gene programs induced by sensory experience. All cell types mounted transcriptional responses to the e...
journals.biologists.com
So happy we finally published this great piece from Antje Keller @antjekeppler.bsky.social and colleagues on the path forward for FAIR bioimage sharing. www.nature.com/articles/s41...
Enabling global image data sharing in the life sciences - Nature Methods
This Perspective provides a roadmap toward globally federated open data resources for bioimaging data.
nature.com
Cool paper about a couple of things I love www.nature.com/articles/s42...
Reversible reduction in brain myelin content upon marathon running - Nature Metabolism
Using magnetic resonance imaging on marathon runners, Ramos-Cabrer, Cabrera-Zubizarreta et al. report that the signal detected as a surrogate of myelin content is significantly reduced after exercise,...
nature.com
Peer review completed within 7 days! Paying peer reviewers! Yes, it's possible. Fast & Fair peer review by @biologyopen.bsky.social More innovation from @biologists.bsky.social Details here: www.biorxiv.org/content/10.1...
Fast & Fair peer review: a pilot study demonstrating feasibility of rapid, high-quality peer review in a biology journal
Traditional peer review is slow, often delayed by the time-consuming process of identifying reviewers and lengthy review turnaround times. This study tests the feasibility of the Fast & Fair peer revi...
biorxiv.org
Biology Open@biologyopen.bsky.social · last yr.We are excited to be launching the next phase of our Fast & Fair peer review initiative: offering high-quality peer review within 7 working days. #fastandfairpeerreview Read the Editorial by EiC Daniel Gorelick @danielgorelick.bsky.social at: bit.ly/4kYD1mL
🚨iGluSnFR4 is finally out!🚨🧪 We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️ www.biorxiv.org/content/10.1... 🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX #Neuroscience
I'm pleased to share our most recent study led by Matija Zelic. We profiled human ALS spinal cords to uncover neuroinflammatory microglia and astrocyte states and show a key role for Ripk1 as a contributor to the pathological tissue environment in ALS. www.cell.com/immunity/ful...
This is awesome! Need immunostaining with two rabbit primary antibodies? No problem, with this approach. We will be looking into this more closely. Great to have such careful validation and optimization work with these tools, thank you Horrocks’ lab!
Christophe 🔬 Leterrier@christlet.bsky.social · last yr.An interesting preprint from the Horrocks' lab detailing the behavior of secondary nanobodies (such a @nanotag.bsky.social ones) at the single molecule level and optimizing protocols for multiplexed labeling: doi.org/10.1101/2025...
