Summer is heating up #FSCWeekend #CryoEM 🌶️🌶️🌶️

Bottomless Krios 😱

Thought I had discovered a new #cryoEM “hole size gradient” feature. Turns out E.coli in ice creates optical illusions ☹️. Or it’s just me hallucinating.

Dear #cryoem community, does anyone if its possible disable atlas categorization in EPU? And if so, how?

I had such a fun time teaching at the U Michigan Cryo-ET Course this week! I taught quantitative ultrastructure analysis and we made cool figures like these! My course materials are at baradlab.com/michigan_tutorial for anyone to try. Workflow include mesh refinement and thickness measurement!

Excited to announce applications are now open for the Wellcome–MRC in situ Cryo-ET Workshop 2026! 📅 28 Sept – 02 Oct 🔬 cryo-ET & FIB (theory + practicals) 📍 @diamondlightsource.bsky.social & @astburycentre.bsky.social + online More info: shorturl.at/LRihg #cryoEM #cryoET #StructuralBiology

A #FIBSEM #cryoET drama in 4 acts. Prelude: Aquilos shuttle rod does not reach cryo stage due to mechanical misalignment.

Here we go again 🙃

Imaging Artifact@imagingartifact.bsky.social · last yr.

Someone please stop the 3DEM auto-reply madness #cryoEM

Introducing Extended PDB IDs at RCSB PDB Search, view, and download all PDB structures using their extended PDB ID. wwPDB anticipates that all four-character PDB IDs will be exhausted by 2028, after which only 12-character PDB IDs will be issued. www.rcsb.org/news/fe...

Introducing Extended PDB IDs at RCSB.org

Search, view, and download all PDB structures using their extended PDB ID. wwPDB anticipates that all four-character PDB IDs will be exhausted by 2028, after which only 12-character PDB IDs will be issued.

rcsb.org

Someone please stop the 3DEM auto-reply madness #cryoEM

First post here- Hello Bluesky! Quick science query: These 2D classes are from negative stain of a FLAG pulldown (HEK cell eluate). Does this look like a real complex or a common contaminant? Would live to hear from anyone who’s worked on similar prep! #negativestainEM #pulldown #HEKcells

Results from CZII's #cryoET object detection challenge on kaggle are in! Participation exceeded our expectations with 931 Teams from 76 countries competing, generating over 27,000 submissions. For an overview check out our post-competition page: cryoetdataportal.czscience.com/competition

Check this out!!! The results from our CryoET particle picking competition are available, the full test data (public and private datasets) is available, and you can dive right into the data on the CZ CryoET Data Portal! Enjoy and please let us know what you think :)

Utz Ermel@uermel.bsky.social · 2y ago

Results from CZII's #cryoET object detection challenge on kaggle are in! Participation exceeded our expectations with 931 Teams from 76 countries competing, generating over 27,000 submissions. For an overview check out our post-competition page: cryoetdataportal.czscience.com/competition

I never felt such joy as I have until I temporarily upgraded to COOT 1, realized how terrible it is, and downgraded back to my old friend Coot 0.9. #CryoEM #TeamTomo

📢 Registration is now open for the @diamondlightsource.bsky.social Biological Cryo-imaging User Meeting 2025! 📍Join us in Nottingham on April 23, in-person & online. Plus, don’t miss the CCP-EM Spring Symposium (April 24-25)🦠#CryoEM #BCIUM2025 🔗Register here: www.diamond.ac.uk/Home/Events/...

The microscope comparison datasets (Glacios vs. Krios, EF vs. no EF) from our high-resolution/high-throughput #cryoEM SBDD workflow project (the one with more than a dozen ligand-bound 2 Å structures of an 85 kDa complex) have been released on EMPIAR: www.ebi.ac.uk/empiar/EMPIA...

Early Christmas present! @mathewmclaren.bsky.social @willstuart.bsky.social @daumlab.bsky.social

Was thinking about RELION5's new coordinate system and came to a realization... Was the switch to a centered coordinate system done to make sure that the coordinates of a particle are applicable to reconstructed tomograms of any size? #teamtomo

For the #ML and #CryoET folks out there: Are folks on here submitting to the CZII CryoET Object Identification competition: www.kaggle.com/competitions... ? The leaderboard is really interesting, and the forums are full of insights. How many of the latest picking models have been submitted?

We received our TFS Arctis this past weekend. This #cryoEM instrument is part of our initiative to expand the #cryoET biomedical research capabilities at the center. SEMC/NYSBC - Providing expertise and resources for understanding both molecular and cellular structures. Find out more at: nysbc.org

The underlying theory (in Fourier space) isn't too far away from that of crystallography. Hopefully the #relion tutorial and the #cryoEM video lectures at @mrclmb.bsky.social's YouTube channel will help too. 🤗

David Dranow@gentlemanscientist.bsky.social · 2y ago

3 hours for an experienced electron microscopist to teach experienced crystallographers how to use RELION... this should be a wild ride!

Cryosparc isn't the only tool you should RELION 🥲 #cryoEM

Harry M. Williams@harrywilliams.bsky.social · 2y ago

To open cryoSPARC all I have to do is type “cry” into the search bar and it comes up. Feels about right. #cryoEM

I'm going to run an intensive "boot camp" called "Practical Cryo-EM" where we learn how to freeze grids, align microscopes, collect images, and do 3D reconstruction and stuff. January 13-17

Greatly enjoying our ongoing #cryoET adventures. My new favourite way to procrastinate from administrative tasks is to play I spy with @bobcoelingh.bsky.social's recent data set! 👀 Here's an easy one! 👇

Anyone have any publicly available data (or data you want to share with me) that you'd love to see segmented? I find myself in need of some fun data to segment in my free time... #cryoET or #volumeEM!

What did you listen to most this year? #CryoEM Reposting this old meme on the new platform

Particle looks like a happy puppers eager to see me. #cryoEM

#TeamTomo What is the most user-friendly template matching software for cryo-ET with samples with rather low complexity? Can't do Warp now without Windows machine.

My first contribution to #TeamTomo . Script to help visualize the subtomogram orientations from #Relion star file inside its tomogram using ChimeraX. Thanks to @AlisterBurt for eulerangles & starfile module. github.com/builab/subtomo…

FYI: Thanks to @cellarchlab.com's awesome open science practices, this data is available on the CZ cryoET data portal (dataset 10008) and @ebi.embl.org's EMPIAR (11058)! You can check out the tomograms in your browser here: cryoetdataportal.czscience.com/datasets/10008

Cryo-electron tomograms of T. kivui of FIB-SEM lamella.

cryoetdataportal.czscience.com

Ben Engel@cellarchlab.com · 4y ago

Excited to share our new study with the Müller and @SchullerJm labs, online @nature: https://www.nature.com/articles/s41586-022-04971-z This is T. kivui, an anaerobic bacterium. It uses hydrogen energy to store #CO2. How does it do it? #CryoET revealed membrane-anchored bundles of...