John Doench
@johndoench.bsky.social
Functional genomics @ Broad Institute. Screen all the things!
New preprint from the group, comparing CRISPRko and CRISPRi via a Perturb-seq readout: www.biorxiv.org/content/10.6...
Direct comparison of CRISPR knockout and interference with Perturb-seq
CRISPR knockout (CRISPRko) and CRISPR interference (CRISPRi) are two workhorse technologies for loss-of-function studies, yet direct comparisons between the two are scant relative to their widespread ...
biorxiv.org
Pete Hegseth Replaces Top General With Horse That Drinks Beer https://theonion.com/pete-hegseth-replaces-top-general-with-horse-that-drinks-beer/
you, a naive fool: i do believe there are some things the nytimes won’t both sides the nytimes:
Breast cancer mortality dropped 58% from 1975 to 2019. Localized, early detected breast cancer has a 99% 5 year survival rate. Modern mammogram tech was developed by NASA for telescopes. Last year, RFK Jr. cancelled 87 research grants on breast cancer. www.nytimes.com/2026/03/16/u...
Susie Wiles, Trump’s White House Chief of Staff, Has Breast Cancer
nytimes.com
I mean, these are always good. But this is So Good. Nitrogen, Ammonia, and the Strait of Hormuz | Science | AAAS www.science.org/content/blog...
Nitrogen, Ammonia, and the Strait of Hormuz
science.org
They made them take down the videos because they would cause "reputational damage" to people like this young man, Nathan Cavanuagh, so definitely don't post this all over the internet with the name Nathan Cavanaugh attached, or this guy might suffer reputational damage. This guy, Nathan Cavanaugh.
404 Media@404media.co · 5mo agoThis is Nathan Cavanaugh, another DOGE staffer explaining how he flagged grants at NEH for "DEI" which would be reviewed for termination. 404 Media has reviewed hours of this footage and we'll have more soon. Part of a lawsuit by @acls1919.bsky.social, @modernlanguage.bsky.social + @historians.org
Holy shit. These people shouldn’t be near sharp cutlery, let alone seats of power. Mullin: “This is war.” Raju: “You concede this is war?” Mullin: “We haven’t declared war.” Reporter: “You just said it’s a war.” Mullin: *stammer
I’ve been to Busytown. I’ve seen these things in action.
Incredible. We got to "Is a pedophile island really all that bad?" five times faster than I thought.
Justin Baragona@justinbaragona.bsky.social · 6mo agoNoah Rothman on the Epstein files: "It is a witch hunt. It‘s a moral panic."
On the unintentional comedy scale, this was 10 of 10 for me.
This style of post is literally every second thing I see on here and it’s utterly absurd. Van Hollen in particular could not be a worse target.
Mary and Joseph praying over Jesus in the manger, or TWO T. REXES FIGHTING OVER A TABLE SAW? Once seen, cannot be unseen.
Drs. Matthew Meyerson and Heidi Greulich from the @broadinstitute.org sit down and discuss how they and Bayer scientists developed the first FDA-approved #CancerDrug to come from a Broad scientific discovery. 🤝 Learn more about their academic-industry alliance: https://bit.ly/4ruQNRl
Magnolia's Cafe on Rochester's deep blue Park Ave might seem like a strange place to meet Trump supporters. But over the clank of metal spoons against bowls of tomato soup, you can hear whispers that the president's friendly meeting with Mamdani may be the beginning of a pivot to the center.
Thank the @johndoench.bsky.social and Root labs for depositing these deliciously named libraries, and check out our blog post for more:
blog.addgene.org
A Tour of Addgene's Most Popular Pooled Libraries
twp.ai
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it! www.fpbase.org/spectra/
FPbase Fluorescence Spectra Viewer
An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.
fpbase.org
First 🟦 post - my main postdoc work is out in the world! We designed a Cas12a-based genetic screen to identify host factors that contribute to infection by the tickborne pathogen Rickettsia parkeri, and stumbled across a really exciting direct interaction between host and bacterium🦠. Check it out!
bioRxiv Microbiology@biorxiv-microbiol.bsky.social · 9mo agoHost cyclophilin-mediated maturation of an obligate intracellular bacterial surface virulence factor https://www.biorxiv.org/content/10.1101/2025.10.28.684717v1
Nature suggests you use their "Manuscript Adviser" bot to get advice before submitting I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
ICE Agent, 7-Year-Old Both Wearing Same ‘Military Commando’ Halloween Costume
When I explain that boycotting an election over an issue has never once forced a politician to embrace that issue, I'm not "scolding people to vote." I'm explaining reality. Feel free to sit on your ass! Just don't pretend that your lazy apathy is somehow going to advance your agenda.
Enjoy car-free small business shopping, music, games, bicycling, community tabling, and more in Jamaica Plain this weekend! 📍 : Centre Street from Lamartine St. to South St. in JP ⏰: This Sunday, November 2 from 10:30 a.m. to 3 p.m. More info: boston.gov/open-streets
This is quite a hed/subhed. 1) Start with Vance. Use strong action verb. Accept the autocratic premise that the “Marines’ Might” are Vance’s to flex. 2) Mention the protest second and absurdly describe them as in the thousands 3) Treat Trump’s strong man use of the military as a partisan scuffle.
Remember, brown 13-year-olds are hardened menaces to society, but 30-something Republican Hitler afficionados in positions of power are just li’l guys.
Clara Jeffery@clarajeffery.bsky.social · 10mo agoThese. Were. Men. In. Their. 30s. www.motherjones.com/politics/202...
New CRISPR libraries from the @johndoench.bsky.social and Root labs — Jacquere (human) and Julianna (mouse). A perfect pairing for your next screen. 🍷🧀 https://twp.ai/9PX2kT https://twp.ai/4irnYP
I have lived and worked in and around Chicago for 27 years and I have been assaulted less often than Sen Paul has been in his own back yard.
Aaron Rupar@atrupar.com · 10mo agoRand Paul: "Chicago is a nightmare. It is literally a war zone."
