For #FluorescenceFriday, an E10 embryo showing Pax2 localization. #DevelopmentalBiology

Our first cancer biology preprint is up! TRIM9 switches melanoma cell phenotypes. First author @klukasik.bsky.social has taken the lab into new directions for sure in a fun collaboration with @coronin.bsky.social. We can't wait to hear what you think. www.biorxiv.org/content/10.6...

Happy to share scHiCAR, an ultra-high throughout (millions of cells), low cost (5 cents/cell including NGS), and trimodal platform for integrated single-cell level analysis of mRNA, open chromatin, and 5-kb resolution looping with ground-truth data the same individual cell.

Nature Biotechnology@natbiotech.nature.com · 6mo ago

Trimodal single-cell profiling of transcriptome, epigenome and 3D genome in complex tissues with scHiCAR - @yaruidiao.bsky.social @dukehealth.bsky.social go.nature.com/4rZqimf

A blue Tulip (Sustentacular cell) behind some red (OMP) OSN cell bodies, and some green horizontal basal cells in the olfactory epithelium. Stylized for a science art presentation (easier to find than original).

Lori O'Brien@llobrienlab.bsky.social · 6mo ago

An old favorite for this #FluorescenceFriday. This "rose" is actually a kidney glomerulus (red, podocytes) and an axon reaching towards it (green).

An old favorite for this #FluorescenceFriday. This "rose" is actually a kidney glomerulus (red, podocytes) and an axon reaching towards it (green).

When your cat is obsessed with #PuppyBowl. As a special needs adoptee herself, she’s a huge advocate.

I am very excited to visit NC State this weekend and give a seminar on Monday! I'll be in Raleigh from Saturday to Tuesday so I'm joyfully accepting all running trail suggestions, dining ideas, cool places to sit and write 6 R01s, and any friends who want to say Hi! gga.ncsu.edu/event/tim-mo...

The beauty of the kidney arterial tree for this #FluorescenceFriday. Evans Blue dye labels the vasculature. Image courtesy of postdoc Sarah McLarnon.

In @elife.bsky.social: A high-resolution, easy-to-build light-sheet microscope for subcellular imaging doi.org/10.7554/eLif...

A high-resolution, easy-to-build light-sheet microscope for subcellular imaging

An accessible light-sheet microscope delivers subcellular-resolution, multicolor volumetric, and live-cell imaging, lowering barriers to state-of-the-art performance.

doi.org

Great things still happen all around us. Each your UNC Biology's Bob Goldstein and Art's Beth Grabowski co-teach ARTS/BIOL 409, that brings together artists and scientists to explore an intersection of two disciplines. It culminated this year with a remarkable show Friday 1/n

Pro tip: NEVER log into My NCBI with more than one 3rd party option. It creates a whole new account that can’t be deleted. I apparently did this once with the ORCiD login (as opposed to normal eRA login) and now I can’t link my ORCiD ID to my SciENcv. I have to ask the Help Desk to merge accounts. 🫠

Introducing Cyclically Multiplexed Expansion Microscopy (Cy-ExM): a workflow for 3D nanoscale, high-plex imaging in whole cells. Cryo-preserved ultrastructure + iterative labeling + expansion microscopy → 20 targets in one dataset with ~70 nm lateral resolution. www.biorxiv.org/content/10.6...

Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy

Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-...

biorxiv.org

Kevin Dean@kevin-dean.bsky.social · 7mo ago

20-plex volumetric super-resolution imaging of cellular ultrastructure. Preprint, led by @seweryn-galecki.bsky.social, dropping soon.

It’s certainly been a year, but I’m incredibly grateful that earlier in 2025 I was promoted to Associate Professor with tenure! Huge thanks to you all for promoting our work here and on the other site, for talk invites, for writing letters of support, and to the lab for all their contributions. 🥂

Unfortunately the drop in ESI funding rates is not paired with changes in funding required to get tenure. It’s not just the next three years of our labs at risk, but the rest of our careers. We are also the cohort that started in the COVID pandemic- it’s been nothing but “unprecedented times”…

Jeremy Berg@jeremymberg.bsky.social · 8mo ago

New story in STAT with data about Early Stage Investigators www.statnews.com/2025/12/18/n... 1/4

Most transcriptomics studies sample ~0.001% of a mouse. Which region do we choose? Often: we guess. Meet DISCO-seq 🤗 flipping the order: RNA-preserving clearing → whole-organ/whole-body 3D imaging → pick ROIs in 3D → unbiased scRNA-seq. Preprint: www.biorxiv.org/content/10.6...

In a paper out this month in @jcb.org we present evidence implicating intrinsically disordered regions (IDRs) as key to junctional assembly and function 2/n 🧪 pubmed.ncbi.nlm.nih.gov/41099653/

Our new work Cilia.io is out on the Rxiv. Ece Atayeter and Jason Ho trained an ML computer vision transformer for detection and quantification of cilia morphology and dynamics. We will provide a GUI soon. Another great collaboration w/ @jbwallingford.bsky.social

bioRxiv Developmental Biology@biorxiv-devbio.bsky.social · 8mo ago

Cilia.io: Computer vision and machine learning reveal spatial patterns of cilia beating dynamics in the spinal cord https://www.biorxiv.org/content/10.64898/2025.12.10.693099v1

🚨 Preprint Friday! 🚨 Thrilled to highlight this collab with David Brown at Duke @cutitoutdave.bsky.social and Ken Poss at UW Madison/Morgridge @kenposs.bsky.social. By comparing 3(!!) regenerating models we identify a conserved role for Sp-family TFs in appendage regeneration (and more).....🧵

Enhancer-directed gene delivery for digit regeneration based on conserved epidermal factors

Limb loss remains a significant clinical challenge, but regenerative medicine approaches such as gene therapy offer a promising strategy to trigger endogenous regeneration programs. Optimal vector con...

biorxiv.org

UNC's Center for Molecular Medicine has up to three open-rank positions for faculty conducting research with relevance to gene therapy, including: novel iPSC and animal models of disease and basic cell biology. Join us in beautiful Chapel Hill! Deadline 1/31/26: unc.peopleadmin.com/postings/309...

Flashback #FluorescenceFriday to some of the first light-sheet imaging we were doing. Video shows the collecting duct system of the developing kidney labeled with a pan-cytokeratin antibody. Courtesy of former talented technician Deanna Hardesty.

🆕 KidneyCure is committing up to $6 million over three years to help eligible NIH R01 applicants ranked in the top 15% continue their kidney research with a $100K award. Learn more and explore every KidneyCure opportunity: https://bit.ly/kcgrantsfunding

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues. www.biorxiv.org/content/10.1...

Sharing this excellent review now out from postdoc Sarah McLarnon! Lots of useful info on 3D imaging, quantification of imaging data, and how this has been applied to the kidney. And since it's #FluorescenceFriday, sharing a figure with our own imaging data! www.sciencedirect.com/science/arti...

For #FluorescenceFriday, RhoA (blue) and the actin cytoskeleton (magenta) are shown in a set of primary microglia 🔬 #Neuroscience #Microscopy

My entry for today’s #FluorescenceFriday: a pupal #Drosophila testis with muscles expressing 🔵 lifeact & 🔴 RFP-nls Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨ 🔗 www.nikonsmallworld.com/galleries/20... #NikonSmallWorld #Microscopy #ScienceArt

For this #FluorescenceFriday, a gorgeous image of an adult mouse kidney labeled with AQP2 and alpha SMA antibodies. AQP2 (green) marks the collecting duct and distal connecting segment while SMA marks the arterial tree. Courtesy of talented postdoc Sarah McLarnon.

Rescued these two beauties today, one stuck in the parking garage stairwell and one from inside the house (and rescued from 3 very interested cats)

Sensory neurons drive pancreatic cancer progression through glutamatergic neuron-cancer pseudo-synapses @cp-cancercell.bsky.social www.cell.com/cancer-cell/...

This is me, one of those 211. My A1 R01 renewal would have been funded otherwise, and I was even initially told they planned to fund it. Now very unlikely. No more chances with this one. We had so many good things going-how do we keep moving forward? A question many of us will be asking.

Prasad Jallepalli, MD, PhD@prasad.bsky.social · 11mo ago

“This isn’t just about 211 missing R01 grants. This is about the 211 PIs (or multi-PI groups) that are not getting a new grant. This is about at least 422-844 non-PI level investigators who are not having their salaries covered.”

How can cells self-organize rapidly into complex patterns during development? Let’s explore a powerful and underappreciated mechanism: Directed Cell Migration (DCM). Preprint @biorxivpreprint.bsky.social : doi.org/10.1101/2025... 👇Thread 🧵(1/11)