Albumin-mScarlet-SpyTag transgenic mice for long-term visualization of blood & interstitial fluid dynamics Hirase lab bioRxiv 2025 www.biorxiv.org/content/10.1... Video GRIN lens imaging of prefrontal cortex in an Alb-mSc-ST (red)🐭 injected with AAV5/CamKII-GCaMP6f (green)

Happy #FluorescenceFriday! 🔥🧪🔬 A blaze of neural crest cells migrates through a developing quail embryo on one of embryonic development's most epic journeys. Beautifully captured by @vanderspuy.bsky.social 👩‍🔬🐣 #Devbio

Another #FluorescenceFriday. When the world is going crazy, I find some peace watching at these myeloid cells (🔴) going about their day. They exist in tissue surrounded by other cells (🔵), and you can see them popping up and down, going in and out of plane as they explore their surrounding.

Happy #FluorescenceFriday! Eye of a zebrafish embryo🐟

New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇

RNA can build. A short RNA self-assembles into a 60-subunit icosahedral cage like a viral capsid, but made entirely of RNA. The striking preprint also reveals a 57-nt RNA filament at ~2.7 Å. Congratulations, Lin Huang and colleagues! www.biorxiv.org/content/10.6...

Nuclei as constellations 🔭 #NucleiSky aligns microscopy images across modalities and scales by reading nuclei like stars to map between them. Joy to contribute, led by @ivanhcenalmor.bsky.social and @guijacquemet.bsky.social's team. #RxivMaker powered preprint. Check it out!

Guillaume Jacquemet@guijacquemet.bsky.social · last mo.

Delighted to share our latest preprint "NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations" www.biorxiv.org/content/10.6... Code and App: github.com/CellMigratio...

Biology was never meant to be studied in 2D. One of the coolest things about my new work is being able to visualize: 🧠 Neural circuits 🩸 Vascular networks 🎯 Drug biodistribution 🎗️ Disease progression Here's a small glimpse of what we're building at @deeppiction.bsky.social 🤩🔬 #3DImaging

Live imaging of immune cell response (🟢) to Salmonella infection (🔵) in the hindbrain of a transgenic #zebrafish larva. Credit to Drs. Jade Leiba & Mai Nguyen-Chi. #ZebrafishZunday 🧪

For this #FluorescenceFriday, some endosomes imaged with MI-SIM. Green: Rab21, Magenta: F-actin, Cyan: EPLINalpha.

A day in the life of a growing hiPSC spheroid expressing endogenously mStayGold-tagged histone 3.3 for #FluorescenceFriday and the #Snouty aficionados. 28 hours (and >50,000 exposures) later and they are still going! Home-brewed volume projection.

Happy #FluorescenceFriday! I captured this shot of a group of embryonic macrophages expressing LifeAct and H2B migrating inside tissue. These cells can use lamellipodia, filopodia, or blebs to migrate, and are frequently switching between these different modes or combining them. So fascinating.

RhoG, Rac1 and Cdc42 cooperation in cell protrusion revealed by multiplexed optogenetics and biosensor imaging by Klaus M. Hahn and team: www.biorxiv.org/content/10.6...

Happy #FluorescenceFriday. I feel so lucky to get to work with these weird and curious primary macrophages from 🐸. They exhibit myriad different morphologies and behaviours, including spinning endlessly and stationary, kinda like progenitor cells under compression from 🐟. Also actomyosin is strong!

happy to share our new protocol paper on wildDISCO for whole-body immunolabeling, tissue clearing & 3D imaging of intact mice at cellular resolution using standard IgG antibodies, for mapping of neuronal, vascular, lymphatic and immune systems across the entire body. www.nature.com/articles/s41...

In their Cell Science at a Glance article and poster, Kim Chi Thi Vu, Laura Bierau and Eva Herker evaherker.bsky.social unimarburg.bsky.social discuss the pro- and anti-viral functions of lipid droplets in infection. journals.biologists.com/jcs/article/...

#FluorescenceFriday From the archives: The Actin Cortex. Plasma membrane (Lck-mScarlet-I 🔴) Cortical actin (GFP-Utrophin 🔵) 1205Lus

Hey y’all 👋 repost magic appreciated. The Solecki lab is recruiting multiple postdocs at St. Jude for a chromatin imaging project at the edge of live-cell imaging, neuronal cell biology, chromatin regulation, and quantitative image analysis. Ever dreamed of touring chromatin like this? We CAN 🔥

Happy #FluorescenceFriday, here is a macrophage engulfing a deformable particle imaged on our LLSM. This project from Mira Krendel's lab and the @aicjanelia.bsky.social just came out as a preprint today! www.biorxiv.org/content/10.6...

happy #fluorescencefriday Compare with last week’s post on APOE3 mice now we have the AD APOE4 model with blood vessels in pink, amyloid in blue and microglia in green. Courtesy of the @mind-western.bsky.social @touchscreencog.bsky.social @tridentpct.bsky.social teams

#microtubules in orange hot, #F-actin greyscale, Fibroblast, spinning disc. @marcoenriquez18.bsky.social @aibnatuq.bsky.social #fluorescenceFriday

🔬 First live-cell imaging experiments done in my lab at HKU! Human RPE1 cells expressing Lifeact-GFP (actin) and EB3-GFP (MT plus-ends), imaged in Fast Airyscan mode on a Zeiss LSM 900👇. Thanks to Denise Kuok Iok Teng for imaging setup, and to Manuel Théry & Louise Bonnemay for the cells 🙏🫶😆

The high-speed logistics of a lung cancer cell. Watch GFP-Rab25 (Gold) move cargo through the internal recycling pathway of A549 cells. Static maps are great, but time-lapse shows the real traffic. Want your science/tech to be highlighted? DM me at Viroscope!

🚨 How do mechanical forces shape a developing organ? Our new #ScienceAdvances @science.org study, led by the amazing @cvagenapantoula.bsky.social, reveals a Piezo1-driven hydraulic mechanism, where mechanical cues control cell volume to guide cardiac formation ❤️🐟 🔗 www.science.org/doi/10.1126/...

Piezo1-mediated mechanohydraulic control of cell volume drives cardiac morphogenesis

Mechanical forces shape the developing heart by controlling cell volume through a Piezo1-driven hydraulic mechanism.

science.org

New perspective on caveolae www.nature.com/articles/s41...

A lipid-centric view of endocytosis by caveolae - Nature Cell Biology

This Perspective discusses the mechanisms underlying both the formation and scission of caveolae, with a focus on highlighting the role of specific lipids in regulating and coupling these processes.

nature.com

It's #FluorescenceFriday and these retinal astrocytes understood the assignment: branching, glowing, and casually laying down the blueprint for the retinal vasculature #Retina #ConfocalMicroscopy

For #FluorescenceFriday, an E10 embryo showing Pax2 localization. #DevelopmentalBiology

Happy #FluorescenceFriday! Just some random data I collected from a while back, actin and myosin in a U2OS cell imaged on our lattice light sheet microscope.