OK— so @phaips.vd.st & @florentwaltz.bsky.social have been testing extensively, and it's pretty clear that MissAlignment may be the best thing to happen to #TeamTomo in the last couple years. One issue: we have to re-process all our data again, hopefully for the last time 😅. Congrats and THANK YOU!!

Continuous recording beam image shift speeds up single particle and tomographic data collection. www.nature.com/articles/s41...

Watch the Tutorial: Introduction to Molecular Animation Learn how to use ChimeraX to create simple animations that can enrich your presentations, websites, and social media

PDB101: Tutorial: Introduction to Molecular Animation

PDB-101: Training, Outreach, and Education portal of RCSB PDB

pdb101.rcsb.org

For years, the #cryoEM community viewed ~50 kDa as the practical lower size limit for SPA. This was challenged by Kim et al., who introduced the HR-HAIR method and demonstrated structure determination of proteins below 30 kDa using #CryoSPARC. Read more 👉 bit.ly/3RHMxAt

CryoSPARC | The Resolution Revolution Is Still Ongoing

High-Resolution Ab-Initio Reconstruction: Extending Cryo-EM to Smaller Particles with CryoSPARC™

bit.ly

It's a small project, but hopefully useful for others running RELION jobs. GitHub: github.com/bfcoop/relio... Feedback and feature suggestions are very welcome! #CryoEM #RELION #StructuralBiology #OpenSource

GitHub - bfcoop/relion_tracker: Track metrics from RELION optimiser STAR files directly in the terminal.

Track metrics from RELION optimiser STAR files directly in the terminal. - bfcoop/relion_tracker

github.com

Hello #world, meet 1,000× Expansion Microscopy. A small gel would grow to the size of an Olympic swimming pool, while amino-acid-scale distances become visible with ordinary light microscopy. Led by Helena Hu from @eboyden3.bsky.social's lab, in collab with us. Story: www.biorxiv.org/content/10.6...

Researchers have identified superparamagnetic macrophages in the livers of rock pigeons to be crucial for magnetic sensing. The finding uncovers an unexpected role for immune cells in sensory perception and may fundamentally change our understanding of animal navigation. https://scim.ag/4nTQ3nq

TL;DR: We've identified more than 100 cases of apparent manipulation in Thermo Fisher Scientific's antibody verification data. @sholtodavid.bsky.social @johanduchene.bsky.social reeserichardson.blog/2026/05/28/h...

How much of Thermo Fisher’s antibody data has been manipulated?

We’ve documented more than 100 instances of apparent data manipulation in Thermo’s catalog

reeserichardson.blog

Don't ignore the "contaminants" on your grids ! We noticed this filament while solving the S. sanguinis T4P structure during my PhD. It turned out to be an incredible eDNA-protease assembly: literally a suspension bridge of proteins & DNA ! 🌉 Happy to finally share this story. #CryoEM #Microbiology

Rémi Fronzes@fronzeslab.bsky.social · 2mo ago

New preprint. In S. sanguinis, a C5a peptidase-like protease (ScpH) polymerizes along eDNA, turning it into a catalytic biofilm scaffold. Great work from @robinanger.bsky.social in our lab, with Pelicic lab @lcbofficiel.bsky.social www.biorxiv.org/content/10.6...

Exciting times for our lab- recent developments in cryoET tools and workflows now allow us apply these to study microbes in the host context. #teamtomo www.sciencedirect.com/science/arti...

Beyond resolution: Cryo-electron tomography of microbial nanomachines in native host context

Cryo-electron tomography (cryo-ET) has revolutionized visualization of bacterial nanomachines by revealing molecular structures in situ under near-nat…

sciencedirect.com

Happy to share our new bioRxiv preprint: “To Kill or not to Kill: A Conserved Trans-Intoxication Protection Factor Blocks X-T4SS-Mediated Fratricide through Interaction with VirB5” www.biorxiv.org/content/10.6...

New Title Alert: AF3Complex- is a model equipped with the same improvements as AF2Complex, along with a novel method for excluding ligands, built on AlphaFold 3. Learn more here: buff.ly/GPEgFSF #SBGrid #Software #Predictions

GitHub - Jfeldman34/AF3Complex

Contribute to Jfeldman34/AF3Complex development by creating an account on GitHub.

buff.ly

Still possible to register for the 14th Young Scientists' Symposium! 📅 4-5 June 2026 @iecb.bsky.social @univbordeaux.bsky.social Fantastic lineup of keynote speakers. @marionjasnin.bsky.social @amjjbonvin.bsky.social @olallalab.bsky.social

📢 Les inscriptions au Young Scientists' Symposium 2026 sont désormais ouvertes ! Pensez à vous inscrire et à diffuser l’information autour de vous 👇 Inscriptions : bit.ly/3OiYTxG Modèle d'abstract pour les présentations orales et/ou posters : lnkd.in/e2x8KJMG

The new ChimeraX CubeNTube plugin allows erasing parts of maps using cube, cylinder, and custom shapes and has undo. Created by Tamino Cairoli. Available from ChimeraX menu Tools / More Tools....

Since last year, I've been making a RELION GUI that supports viewing, navigating and running jobs in a way completely compatible with the original RELION pipeline. Now it's working nicely for both SPA and tomography! Please try it out - github.com/hanjinliu/hi...

🔬 Just out in @NatureComms! Using cryo-CLEM and cryo-ET, we reveal the unique ultrastructure of dopaminergic varicosities in the striatum. Amazing collaboration with @davip-bdx.bsky.social and Etienne Herzog @iins-bordeaux.bsky.social Congratulations to Paul and Robin for this fantastic work! 🎉

Very happy to share our work on lipopolysaccharide assembly by the Lpt complex, published today in @natcomms.nature.com Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social Congrats to all authors, especially Haoxiang, Axel & Violette 🔗 doi.org/10.1038/s414...

Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex - Nature Communications

The asymmetric distribution of lipopolysaccharide (LPS) on the surface of the bacterial outer membrane is essential and crucial for antibiotic resistance. Here, authors characterize the LPS translocon...

doi.org

Our latest T7SS study is now out in Science Advances! We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery. www.science.org/doi/10.1126/...

A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system

Cryo-EM structure reveals how a ubiquitin-like protein orchestrates assembly of a bacterial weapon system.

science.org

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit www.nexperion.net/semcm2026

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

Philippe Van der Stappen@phaips.vd.st · last yr.

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

We're on the cover of @science.org this week. Awesome work by @florentwaltz.bsky.social. #CryoET is reaching crazy resolutions inside cells, and this is just the beginning. Fantastic interpretation of the proton motive force by @verenaresch.bsky.social. Always a pleasure making #SciArt with you!🧪🧶🧬🌾

Science Magazine@science.org · last yr.

In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion. Learn more in this week's issue: scim.ag/3FA3Ygq

ChimeraX can be used to find pockets and cavities in protein structures using the Find Cavities tool or kvfinder command. This feature is available in daily builds from 10 March 2025 or later.

AreTomoLive: Automated reconstruction of comprehensively-corrected and denoised cryo-electron tomograms in real-time and at high throughput www.biorxiv.org/content/10.1101/2025.03.11.642690v1 #cryoEM

Stand up for science in Bordeaux

Cryo-iCLEM: Cryo correlative light and electron microscopy with immersion objectives pubmed.ncbi.nlm.nih.gov/39971020/ #cryoEM

🔬Our @haselbachlab.bsky.social with Pavol Harar have developed ‘FakET’! The new method creates ‘fake’ electron microscopy images to train AI, reducing manual work in particle identification. Read the full story: www.imp.ac.at/news/article... @viennabiocenter.bsky.social #AI #cryoem #microscopy

Are you using @napari.org or want to learn more? I've curated a list of videos about napari and its uses on YouTube. If I missed a video, please let me know. www.youtube.com/playlist?lis...

napari - scientific image viewer - YouTube

napari (https://napari.org) is a fast, interactive viewer for multi-dimensional images in Python. Napari is a Python library for n-dimensional image visualis...

youtube.com