Excited to share our new review! 🎉 Together with @rhuttenhain.bsky.social, we discuss how emerging proteomics approaches are revealing the spatial and temporal organization of the cellular proteome. Great fun writing this together! www.sciencedirect.com/science/arti...

Resolving cellular signaling in space and time: From organelle proteomics to spatial phosphoproteomics

Cellular signaling is inherently organized in space and time, requiring coordinated control of protein localization, molecular interactions, and enzym…

sciencedirect.com

Ruth Huttenhain@rhuttenhain.bsky.social · 4w ago

Excited to share our new review in Current Opinion in Chemical Biology! We discuss how advances in proteomics are transforming our understanding of where, when, and how signaling occurs in cells. Thanks to @alexmatia.bsky.social for writing the article with me. www.sciencedirect.com/science/arti...

Our new paper on a rapid, reliable way to make induced astrocytes for co-culture with neurons is out on bioRxiv: www.biorxiv.org/content/10.6... We hope this system will be a useful tool for illuminating the pathways underlying neuron-astrocyte interactions. More to be shared soon!

iAstrocytes model cytokine influences on complement expression and neuronal network synchronization.

Astrocytes play essential roles in neuronal development, function, and disease, yet existing methods to derive astrocytes from human pluripotent stem cells (hPSCs) are complex and can involve months o...

biorxiv.org

Our Year-End Symposium and Research Showcase is tomorrow! We are excited to host esteemed speakers in the field, including Nir Barzilai (@nirbarzilaimd.bsky.social), Anne Brunet (@brunetlab.bsky.social), Takeshi Uenaka (@tuenaka.bsky.social), Ivan Soltesz, and Katrin Andreasson.

🧵New paper from the lab @isd-research.bsky.social @lmu-klinikum.bsky.social @lmu.de in STM @science.org! A human #iPSC-derived #Tauopathy model that endogenously develops late-stage Tau pathology. Free personal PDF: www.science.org/eprint/5H5YD.... Here's what we found👇 1/10

Only smiles in the tissue culture room from now on 🙂 Find the TK4 plasmids from the Wernig lab here: www.addgene.org/browse/artic...

Takeshi Uenaka@tuenaka.bsky.social · 5mo ago

Have you ever cried in the tissue culture room because your #iPSC transgene got silenced after differentiation? I have. Many, many times. So we built TK4, a PiggyBac vector designed to resist silencing. Now available on @addgene.bsky.social: #239047 / #239046 Would love to hear your thoughts.

Have you ever cried in the tissue culture room because your #iPSC transgene got silenced after differentiation? I have. Many, many times. So we built TK4, a PiggyBac vector designed to resist silencing. Now available on @addgene.bsky.social: #239047 / #239046 Would love to hear your thoughts.

Losing sleep over #iPSC transgene silencing after differentiation? Same here. Bright in iPSCs. Gone after differentiation. Our paper in @cp-cellstemcell.bsky.social maps what actually keeps expression on👇 www.cell.com/cell-stem-ce... 🔗 Free access (50 days): www.sciencedirect.com/science/auth...

Thrilled to see our paper out in @cp-cellstemcell.bsky.social!

Cell Stem Cell@cp-cellstemcell.bsky.social · 6mo ago

Online Now! Prevention of transgene silencing during human pluripotent stem cell differentiation #stemcells

Our paper on preventing transgene silencing is now online in @cp-cellstemcell.bsky.social! 🔗 Free access (50 days): www.sciencedirect.com/science/auth...

Our paper is (finally) out in Cell today! CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis www.cell.com/cell/fulltex... Great collaborative effort - read more from first author @asamelson.bsky.social below:

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

CRISPR screens in iPSC-derived neurons reveal that the E3 ubiquitin ligase CRL5SOCS4 ubiquitinates tau, that CUL5 expression is correlated with resilience in human Alzheimer’s disease, and that electr...

cell.com

asamelson@asamelson.bsky.social · 6mo ago

After a long review process, I'm excited that our paper is finally in print: www.cell.com/cell/fulltex... TL;DR: We use CRISPR screens in iPSC-derived neurons to find a new tau E3 ligase and a relationship between oxidative stress, the proteasome, and tau proteolytic fragments. More below 👇

New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...

Astrocyte induction of disease-associated microglia is suppressed by acute exposure to fAD neurons in human iPSC triple cultures

Lish et al. present a co-culture system of iPSC-derived human microglia, astrocytes, and neurons to investigate intercellular communication across different environmental and genetic contexts. Transcr...

cell.com

Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research! www.biorxiv.org/content/10.1...

Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation

While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occu...

biorxiv.org

Silencing of transgenes during iPSC differentiation is a frequent problem. 🧬🫢 Check out the piggyBac system below, which has resisted silencing in many protocols across many labs. 💪 Excited that our lab could contribute to this team effort led by @tuenaka.bsky.social & Marius Wernig!

Takeshi Uenaka@tuenaka.bsky.social · last yr.

Losing sleep over #iPSC transgene silencing after differentiation? Same here — too many times😭 So we built a PiggyBac construct that resists silencing. Expression stays ON, even after differentiation. Our #preprint is out👇 🔗 www.biorxiv.org/content/10.1... Supported by @cziscience.bsky.social

Losing sleep over #iPSC transgene silencing after differentiation? Same here — too many times😭 So we built a PiggyBac construct that resists silencing. Expression stays ON, even after differentiation. Our #preprint is out👇 🔗 www.biorxiv.org/content/10.1... Supported by @cziscience.bsky.social

Prevention of Transgene Silencing During Human Pluripotent Stem Cell Differentiation

While high and stable transgene expression can be achieved in undifferentiated pluripotent stem cells, conventional transgene expression systems are often silenced upon differentiation. Silencing occu...

biorxiv.org