#cryoet #teamtomo

Biohub@biohub.org · 2mo ago

In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn

In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn

ps. Interested in alternative ways to use LM data? Check out preprints from: Vojta Pražák + colleagues: www.biorxiv.org/content/10.6... Giulia Zanetti’s group: www.biorxiv.org/content/10.1... Both show how LM tomos can guide HM acquisition when events of interest are hard to spot from search maps

Huge thanks to Michael Grange (@michaelgrange.bsky.social), Fabian Eisenstein (@eisfabian.bsky.social) , and Victoria Garcia-Giner — really grateful for all their help and support on this work!

Would love everyone to give it a go! Cryo-ET is already a great tool to study structures in context — interleaved multi-magnification acquisition captures even more of that context, without compromising throughput or STA resolution Excited to see what people do with BIGSMALL! (6/6)

BIGSMALL is now implemented in SPACEtomo. Setup is easy: - define low mag parameters with SerialEM Search preset - add LM + HM targets in the same beam-shift group - acquire! (5/6)

BIGSMALL interleaving means there are minimal downsides: ✔️ Maintains throughput (no added stage movements) ✔️ Preserves high-resolution data We reach 3.7 Å for the 𝘌. 𝘤𝘰𝘭𝘪 50S subunit in both interleaved and conventional acquisitions. (4/6)

Cryo-ET isn’t just about high-resolution structures. Low-mag data lets you analyse cell biology across much larger spatial scales. We show this by mapping fenestrations in the RER across extended regions in 𝘊. 𝘦𝘭𝘦𝘨𝘢𝘯𝘴. (3/6)

BIGSMALL captures both high-resolution structures and large-scale cellular context at the same time, by acquiring high- and low-magnification images at every tilt in a tilt-series. This gives: 🟩 large FOV, low mag tomos 🟥 high res, high mag tomos from the same sample region (2/6)

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

CUNY and SUNY colleagues: ICYMI Please sign and share this letter to the NY Congressional Delegation asking them to protect NIH and NSF funding! docs.google.com/forms/d/e/1F...

New York's Congressional Delegation Must Defend Federal Research Funding for CUNY & SUNY. List of signatories.

FACULTY & STAFF: ADD YOUR NAME BELOW TO THIS OPEN LETTER WRITTEN BY THE DEFENDING RESEARCH FUNDING WORKING GROUP OF THE PROFESSIONAL STAFF CONGRESS/CUNY Federal investment in scientific research gene...

docs.google.com

Savage-Dunn lab@cswormlab.bsky.social · last yr.

CUNY and SUNY colleagues - join us in signing this open letter to the NY State Congressional Delegation! docs.google.com/forms/d/e/1F...

MIT News reports on focused research organizations (FROs), non-profit startups, developed by some of our group members and alumni. FROs can work on problems that are a poor fit for academia or the for-profit startup world. news.mit.edu/2025/former-...

Former MIT researchers advance a new model for innovation

Developed by former MIT researchers, focused research organizations (FROs) undertake large research efforts and have begun to contribute to scientific advances.

news.mit.edu

The amazing Sofia Lövestam initiated the below project, when she became interested in the vault particles that we sometimes observe in #cryoEM images of brain-derived #amyloid filaments. www.biorxiv.org/content/10.1...

Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps

The vault protein is expressed in most eukaryotic cells, where it is assembled on polyribosomes into large hollow barrel-shaped complexes. Despite its widespread and abundant presence in cells, the bi...

biorxiv.org

This isn’t just numbers on a table–it’s my lab and the labs of my colleagues. Terminated grants mean shuttered projects, lost talent, and stalled progress against deadly pathogens. The damage to science–and to public health–is real and immediate. Thanks @peterhotezmdphd.bsky.social for speaking out.

Prof Peter Hotez MD PhD DSc(hon)@peterhotezmdphd.bsky.social · last yr.

Devastating NIH cuts to infectious disease research, eviscerating our nation’s capacity to fight emerging infections and pandemic threats. From @sanders.senate.gov report out yesterday www.sanders.senate.gov/wp-content/u...

Back to @mblscience.bsky.social Woods Hole - teaching on the Pair Up Microscopy Course. Great experience and awesome participants. Wild - last time here I was a student (Physical Biology of the Cell Course in 2018). Now introducing my lab. Thanks Abhishek Kumar for organising and the invitation.

If people of means are interested in establishing a 2025 “Institute for Advanced Study” for displaced/defunded biomedical scientists, I know many people who will help, including myself. @mcuban.bsky.social @arcadiascience.com @arcinstitute.org

John Skiles Skinner@skiles.blue · last yr.

Let's talk about these two department store owners: brother and sister Louis Bamberger and Caroline Bamberger Fuld. From Newark, New Jersey. They are random people, basically. But in the late 1920s, with fascism taking over Europe, they made a choice that profoundly changed the world: 🧵

Just found out my two related NSF grants (on discovery of phages that target antibiotic resistance vectors and building synthetic systems to understand the biology of those vectors) were also caught in Harvard’s mass grant termination

Here’s another spectacle of New York spring entomology: Eastern tent caterpillars (Malacosoma americanum) are among the most social lepidopterans. In May, you can find their silken tents, in which they aggregate (and defecate).

They have three daily feeding bouts, during which they exit the nest and move together to distant feeding sites. They mark their route with silk and trail pheromone. Successful “scouts” can even recruit the colony to new food sources. They’re kind of the ants among the butterflies. Woodstock, NY.

My lab at NYU is hiring a postdoc interested in RNA biology and local translation in neurons following axonal injury/neurodegenerative diseases!! Apply here: apply.interfolio.com/165681

Tomorrow at the Systems Virology Journal Club, @tamanash.bsky.social will present his beautiful work with @harmitmalik.bsky.social on optimizing temperature-dependent trade-off between protein production and processing in alphaviruses through stop codon selectivity pubmed.ncbi.nlm.nih.gov/40249804/

Shira Weingarten-Gabbay@shiraweingarten.bsky.social · 2y ago

@wmschneider.bsky.social and I are thrilled to invite you to the 7th series of the International *Systems Virology Journal Club*! 🎉 🥂🌟 We are hosting a stellar line-up of speakers working at the intersection of virology and systems biology. Registration (free): forms.gle/edTyLwR8jWkh...

Groundbreaking study in Cell from @leventallab.bsky.social: phospholipid asymmetry is a defining feature of the plasma membrane and cholesterol fills the holes — major implications for how this membrane works. A #lipidtime must-read! www.cell.com/cell/abstrac...

Cell membranes sustain phospholipid imbalance via cholesterol asymmetry

This work challenges a major assumption in cell biology by showing that lipid bilayers can have drastically different phospholipid abundances between their two leaflets. This lipid abundance asymmetry...

cell.com

Rachael, thank you for this. We can all benefit from the example of your courage and wisdom in speaking out.

Rachael Sirianni, PhD@docsirianni.bsky.social · last yr.

Hey Bluesky, I was interviewed about the attacks on science for NPR’s All Things Considered. Listen, below! www.wbur.org/news/2025/03...

Structural insights into the coupling between VCP, an essential unfoldase, and a deubiquitinase. New study from Lauren Vostal, Tarun Kapoor and colleagues @rockefelleruniv.bsky.social: rupress.org/jcb/article/... #StructuralBiology #Biochemistry #ProteinHomeostasis #CryoEM

WE GOT THE COVER!!!

Science Magazine@science.org · last yr.

In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion. Learn more in this week's issue: scim.ag/3FA3Ygq

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨

Science Magazine@science.org · last yr.

In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion. Learn more in this week's issue: scim.ag/3FA3Ygq

Congratulations to first authors @rufeili.bsky.social and @tshindmarsh.bsky.social !!!!

Here is the paper, worth a read for those interested in behavior, neuroscience, and mating competition: www.cell.com/cell/fulltex...

Proud that one of my photographs has made the cover of Cell. (first 'official' photograph publication) Very happy to contribute the pic and highlight the latest publication of the Ruta lab.

In preparation for the lecture tomorrow, I opened "The Cell in Development and Heredity" by E.B. Wilson (Columbia Univ), and realized that this 3rd edition was published in 100 years ago. So many things happened since then, including the fact that I, a Japanese Prof in NY, own and read the book.