Mitochondrion on a block of walnut. I went all out on the sanding to make it super smooth on this one.

A small joy is to find a paper from the 80’s explaining something that seems to have somehow been forgotten.

any recommendation for a high throughput imaging system to sort zebrafish larvae based on fluorescence? Thinking about the VAST or AcquiferIM or..... Any input welcome!! #zebrafish

Believe it or not we have addressed this! Here you go: single gar = gar multiple gars, same species = garrison multiple gars, different species = garmada

Frank Hogan@charleyphogg.bsky.social · last wk.

A group of Gar can not be called a school it’s not tough enough

St. Jude scientists identified sub-compartments in the nucleolus that help lock ribosome building blocks in place. These findings highlight the role played by phase separation, shedding light on diseases linked to increased ribosome production. bit.ly/4bK3SzL

Research explains how nucleolus sub-compartments drive ribosome assembly

See how RNA and SURF6 sub-compartments surrounded by NPM1 help in ribosome biogenesis within the nucleolus granular component condensate

stjude.org

Alright I gotta go run a potentially soggy moth night Do me a kindness and close some states while I'm out talking urban biodiversity with my fellow Philadelphians 🥺 $10 to fill a bubble. Fill this map in: givebutter.com/USS2026

United States of Science 2026

By Skype a Scientist

givebutter.com

Sarah McAnulty, Ph.D.@sarahmackattack.bsky.social · last mo.

The 2026 United States of Science Challenge starts NOW! Each bubble represents classrooms we’ve matched with scientists this year. This a competition to fill bubbles with $10 donations to support @skypeascientist.bsky.social The first region to fill all bubbles wins. 👇🏻 givebutter.com/USS2026

I feel like I am echoing the editorial, but the Points of View and Points of Significance series were very influential to my scientific journey. I am thrilled to see Points of View come back, being helmed by @helenajambor.bsky.social who has already done great work amongst the microscopy community

Nature Methods@natmethods.nature.com · 2mo ago

We are very excited to bring back our popular Points of View column, written by @helenajambor.bsky.social! Check out her first column in our July issue about the usage of color scales in scientific figures. www.nature.com/articles/s41...

The #ThermoFisher #antibody scandal is an ugly reminder, I think, of how few people (including those at TF, it seems) are trained to properly validate antibodies before use. Some advice on antibody validation in the posting 👇 totalinternalreflectionblog.com/2022/09/18/h...

Hitting the target (a short guide to validating antibody specificity)

Antibodies are essential and ubiquitous tools in biology, but their unguarded usage can easily lead to error. Here’s how to validate their specificity, and ensure that they’re hitting the rig…

totalinternalreflectionblog.com

Reese Richardson@reeserichardson.bsky.social · 2mo ago

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

Oh and of course the editorial that was not supposed to be shared: diabetesjournals.org/care/article...

Misguided Brushes of a Pen Continue to Dismantle and Destroy Biomedical Research in the United States: We Can No Longer Afford Complacency and Fear. We Must All Act Now!

The opinions expressed in this editorial are the personal views of the authors (S.E. Kahn, C.A.M. Anderson, J.B. Buse, and E. Selvin) and do not represent

diabetesjournals.org

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

Run controls. Always. Anything short of that isn't science. Full stop.

Update on GeneFrame alternative for bacterial imaging. After discussion with us and others, SunJin lab have how launched a substitute for timelapse imaging in geneframes www.sunjinlab.com/product/ispa... Dimensions are very similar, price is reasonable ($105 for 250) #microsky

Séamus Holden 🔬🦠🧫@seamusholden.bsky.social · last yr.

Fellow bacterial cell biologists, message me if you use @thermofishersci.bsky.social Gene Frames for microscopy! Thermo are planning to discontinue these in September but have indicated they will look into this further. If I make list of labs using them Thermo might not kill the product Please RT🔬🦠

Thermo Fisher has responded to research integrity activists who spotted apparent manipulation in its antibody catalog: "In ... preparing antibody images for publication on our website, some images may have been adjusted to clarify for presentation purposes." More: cen.acs.org/research-int...

translated: we used faked results to try and convince you to buy our over-priced antibodies, and then offer a set of lies to justify it

Laurel Oldach@laureloldach.bsky.social · 2mo ago

Thermo Fisher has responded to research integrity activists who spotted apparent manipulation in its antibody catalog: "In ... preparing antibody images for publication on our website, some images may have been adjusted to clarify for presentation purposes." More: cen.acs.org/research-int...

Check out my "rough draft" of my instrument status page for the HCBI. Any feedback before I set it up on an HCBI GitHub? hbrown227.github.io/lab-instrume...

Combining biosensor imaging data with an animated plot of the quantified intensity, based on a blog I wrote earlier: thenode.biologists.com/crafting-plo... In this version I used a dark theme

#BlueSci question. Any recommendations for an (anti-rat) NIR 2y Ab to be used with a 730 nm laser line? The 2 obvious choices are AF750 or DyeLight755, with (on paper) AF750 brighter? Not sure about comparative photostability? No appropriate JaneliaFluors? @christlet.bsky.social any thoughts?

I think it would be cool to link up all the Imaging/Analysis YouTube Channels in the "Featured Channels" section of HCBI's YouTube homepage. What are some of the best we could highlight?

Some confetti to celebrate #FluorescenceFriday

What is the public opinion on color coding treatments in figures and then using the same colors used in the graph to outline matching representative images? Is it useful or am I being too extra?

Happy #FluorescenceFriday all! Checkout these beautiful basal bodies in airway #multiciliated cells expanded via U-ExM 😍 Images captured by the super talented @maneesha07.bsky.social on a #Nikon NSPARC 🔬.

Anyone (in the US) have strong opinions on what companies do the best fabric posters?

#HappyFluorescenceFriday #microscopycommunity - into Expansion Microscopy? Come to the Expansion Microscopy User Group Community Discussion Series! First session, Tue, March 10, on “Expansion Microscopy in Practice Series: Choosing the Right Protocol” Learn more: buff.ly/V4umk5E

St. Jude researchers and collaborators developed M-PACT, a tool that leverages AI to classify pediatric brain tumors using cell free DNA from cerebrospinal fluid. The approach could improve diagnosis and disease monitoring when tissue biopsy is not feasible. ow.ly/aUcU50Yh2bb

Classifying pediatric brain tumors by liquid biopsy using artificial intelligence

See how M-PACT, an AI-powered algorithm can sift through ctDNA in cerebrospinal fluid and molecularly classify tumors based on their DNA methylation pattern

ow.ly

Anyone ever tried super glue (cyanoacrylate) for mounting coverslips with stained mammalian cells, to be used for fluorescence microscopy? I'm looking for ways to conserve microscopy samples for a looooooong time...

A loaded question to scream about...which agarose do you use to mix your beads to measure the PSF? Any brand #lsfm #lightsheet

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

Quantitative Imaging: From Acquisition to Analysis

Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

meetings.cshl.edu

Defending PhD student, looking over their thesis: “If I knew then what I know now, I could’ve done all of this in like 9 months.” A thread about my favorite pioneering cave explorers and why I don’t think AI will ever “solve” biology.