John Rubinstein
@johnrubinstein.bsky.social
CryoEM, Bioenergetics, V-ATPases (especially in synaptic vesicles and lysosomes), OxPhos (especially in mycobacteria as a TB drug target) Scientist, The Hospital for Sick Children Professor, U of Toronto 👨👩👧🔬🚲 rubinsteinlab.org COI: structura.bio
Introducing CsMT: A new CryoSPARC workflow for high-resolution microtubule reconstruction based on novel PF-pair classification! You can reconstruct MTs entirely in CryoSPARC—zero custom scripts needed. Inspired & built through a fantastic collaboration with the Cianfrocco lab! tinyurl.com/kdpm86tw
Please repost! #microsky🦠 The Glickman Lab at MSK is recruiting! Projects in Mtb pathogenesis, basic biology of mycobacteria, and mycobacterial cancer therapies. See our publications here: tinyurl.com/3f386f4x Send CV and cover letter to: glickmanmycolab@gmail.com
scholar.google.com
Huge congratulations to Professor Jacob Tsimerman of the University of Toronto on receiving the Fields Medal — one of the highest honours in mathematics. Nearly a century after Canadian mathematician J.C. Fields established this award in Toronto, it comes home.
🚀 Can automation solve preferred orientation in your #cryoEM datasets? After automating processing across 21 GPCR datasets in #CryoSPARC, we updated the workflow to be even more robust to preferred orientation, achieving improved map quality over manual processing. Read more 👉 bit.ly/3RyOIGG
📢 Super excited to promote that our #Immunology department is recruiting a Tier 2 Canada Research Chair in Immunobiology of Chronic Disease!🎉🥳 Please note this is a targeted recruitment with more details via QR code. I am lucky to work here in such a collaborative environment Please share widely
✉️ The latest #CryoSPARC Newsletter is out! Highlights include: 🔹 Sept 2026 workshop hosted with SickKids 🇨🇦 🔹 New cryosparc-tools video 🔹 New case study on discrete heterogeneity 🔹 Small-protein #cryoEM advances 🔹 Dynamic masking guide (v5) Read more here: mailchi.mp/b8fb173dac5c...
CryoSPARC workshop in Toronto! Beyond consensus refinements: Advanced cryo-EM data processing with CryoSPARC Sept 24-25, 2026 (part of the Canadian Cryo-EM Nexus) Register here: cvent.me/dL1wem Please see details in the link or the image Alt text @structurabio.bsky.social @sickkidsto.bsky.social
CryoSPARC workshop in Toronto! Beyond consensus refinements: Advanced cryo-EM data processing with CryoSPARC Sept 24-25, 2026 (part of the Canadian Cryo-EM Nexus) Register here: cvent.me/dL1wem Please see details in the link or the image Alt text @structurabio.bsky.social @sickkidsto.bsky.social
New preprint! Karin Schumacher's postdoc Nadja used our SidK approach to purify Arabidopsis V-ATPase & Mariia Khamina determined its structure. The structure shows unique plant V-ATPase features and that TLDc proteins (here OXR5) bind V-ATPase in all eukaryotes. 🌱 tinyurl.com/mw9xaszk
🚨 We are recruiting at the Ottawa Hospital Research Institute, in the Regenerative Medicine Program ! If your are passionate about basic science, and dream to develop your own research program, please apply! #Ottawa #Canada #Recruitment #Scientist #Research Full description at the link below:
Tier 2 Canada Research Chair in Vascular Links to Neurodevelopment
Follow us on LinkedIn Posting Reason: New Position Job Type: Contingent Worker Anticipated Duration in Months (for contracts and temporary assignments): Job Family: Academic Leadership and Faculty # o...
uottawa.wd3.myworkdayjobs.com
📢 We're hiring! We are looking for an Assistant or Associate Professor to join our team at Utrecht University. If you're excited about leading an independent research programme in a collaborative #virology community, we'd love to hear from you. Please share! 👇🦠 #AcademicJobs #FacultyPosition
Thankfully quarantine for MV Hondius cruise passengers just ended without any new cases, but it is always a good time for Molecule of Month to explore how a family of rodent-borne viruses can cause severe illness in humans...
PDB101: Molecule of the Month: Hantavirus
Family of rodent-borne viruses that can cause severe illness in humans
pdb101.rcsb.org
Incredibly proud of Messi Haile @mehsehret.bsky.social, a founding member of the Ho Lab, for her beautiful work uncovering the inner workings of a central piece of the malaria parasite invasion machinery: the malarial moving junction, out today in @cellpress.bsky.social! tinyurl.com/4p3md2eb 🦠❄️🔬
Our evil ORFeome paper is out! In collaboration with @alex-stark.bsky.social, we screened ~4,000 viral proteins and secreted effectors from bacteria and parasites, covering hundreds of diverse pathogens, for phenotypes in human cells.
Honored to be elected to EMBO,together with Manu, Julian and ex-benchmate @wojtekgalej.bsky.social. I’m grateful to all past and present Iab members for their amazing work and support from colleagues, mentors and family over the years.
MRC Laboratory of Molecular Biology@mrclmb.ac.uk · last mo.Congratulations to LMB Group Leaders Emmanuel Derivery, Kelly Nguyen and Julian Sale, who have all been elected members of @EMBO.org! Read more about their research at the LMB here: mrclmb.ac.uk/news-events/... #LMBNews @deriverylab.bsky.social @kellythd-nguyen.bsky.social @juliansalelab.bsky.social
Come join the new Novo Nordisk Foundation Center for Protein Design as a group leader Cool science in a great scientific environment in wonderful and very family friendly Copenhagen There are two positions that can either be at TT assistant or associate prof level employment.ku.dk/tenure-track...
Tenure-track Assistant Professor or Associate Professor of De novo Protein Design
The Novo Nordisk Foundation Center for Protein Design at the University of Copenhagen seeks to appoint two new group leaders in de novo protein design from 1 February 2027 or as soon as possible thereafter. These positions will be at the Assistant or Associate Professor level and associated with the Department of Biology (Faculty of Science) or the Department of Drug Design and Pharmacology (Faculty of Health and Medical Sciences).
employment.ku.dk
Our biology department at Université Laval has a faculty position in Cell Biology, Molecular Biology, or Genetics. We have an Institute of Integrative and Systems biology, we are unionized, and Québec City is a wonderful mix of culture and access to nature. AMA universityaffairs.ca/search-jobs/...
Search Jobs - University Affairs
universityaffairs.ca
Probably the best thing I ever posted on that other site, wish I could permanently transplant it to my timeline here.
For years, the #cryoEM community viewed ~50 kDa as the practical lower size limit for SPA. This was challenged by Kim et al., who introduced the HR-HAIR method and demonstrated structure determination of proteins below 30 kDa using #CryoSPARC. Read more 👉 bit.ly/3RHMxAt
CryoSPARC | The Resolution Revolution Is Still Ongoing
High-Resolution Ab-Initio Reconstruction: Extending Cryo-EM to Smaller Particles with CryoSPARC™
bit.ly
Janet Rossant to receive #UofT honorary degree for her world-renowned research & leadership in biomedical sciences #UofTGrad26 📜 uoft.me/crf
We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇
Thrilled to announce that the Department of Microbiology at the University of Washington, School of Medicine is hiring at the Assistant Professor level (tenure-track)! The Pacific Northwest is an awesome place to live and do science 🧪🦠. Join us! apply.interfolio.com/185622 Applications due 8/1.
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Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs doi.org/10.1038/s415...
🔬 We’ll be at the 6th NY Area #CryoEM Meeting on May 27, presenting recent developments in #CryoSPARC for Homogeneous Ab Initio Refinement and workflows for small proteins. bit.ly/4dYMUze We’re also proud to support the meeting as a sponsor — stop by our poster to learn more!
Long shot, but, with lots of labs turning from crystallography to cryo-EM I’m wondering if anyone knows of labs potentially looking to offload some of their old crystallography supplies to a PUI with potential screening hits and really eager students but nothing to harvest with, etc.?
One, perhaps two, of our postdocs are moving on to faculty positions this year (more news soon!). While they're truly irreplaceable, it does mean that we have openings for new members to join our team studying #cilia. If interested, please apply: academicpositions.harvard.edu/postings/15526
