🔬🧫 Biology friends. Who do you outsource cell line CRISPR editing to? We could do in my lab, but given people and time constraints I want to explore commercial $ to edit standard hES cell line - KO/cKO. Bonus if work is done in USA and not shipped to international facility.

SML Chef Pee Pee: The Crisper, The Better

Alt: SML Chef Pee Pee: The Crisper, The Better

static.klipy.com

Twenty years ago, Addgene co-founder Melina Fan, PhD, couldn’t get the plasmids she needed for her research, so she helped build a better way to share them. 🧬 On Aug. 1, Melina stepped into a new role as Chief Scientific Advisor. A thank-you and a look at what’s next:

blog.addgene.org

Thank You, Melina: A New Chapter After 20 Years

twp.ai

The reputation-laundering operation known as AlphaFold has been shut down. It changed culture in damaging ways, and similar protein-structure packages carry the mantle of black-box short-cuts that break the "chain of custody of why", offloading labor to often-haphazard and de-emphasized validation.

Google shuts down its Nobel-prize winning AlphaFold project as it focuses on Gemini - Engadget

DeepMind's award-winning AlphaFold team is no more.

engadget.com

Jed Brown@jedbrown.org · last mo.

The "chain of custody of why" is a load-bearing element of science. In numerical solutions of PDE (as in fluid and structural mechanics), the modern practice of verification (solving the equations right) and validation (solving the right equations) as distinct emerged from painful epistemic flailing

It's been a long time coming, but I'm so proud of my amazing wife @erinduffyphd.bsky.social starting her lab at Brown next year 🎉 Follow her for all things RNA in the brain! 🧠🧬

Erin Duffy, Ph.D.@erinduffyphd.bsky.social · 2w ago

The Duffy Lab is coming July 2027!! I’m thrilled to announce that I’ll be joining the Center for Alzheimer’s Disease Research at Brown University as an Assistant Professor of Molecular Biology, Cell Biology, & Biochemistry.

One of my teacher friends often says that people confuse "few people are able to do x" with "x reveals who is good" and I think about that a lot

The STRAIGHT-IN (Dual) kit is now available via @addgene.bsky.social! 🧬 A complete 24-plasmid toolkit for installing up to two orthogonal CLYBL landing pads, DASIT-based enrichment without FACS, and efficient large DNA integration in human cells. Link: www.addgene.org/kits/gallowa... 🧵

Plasmid errors are more common than expected, and not every method catches them all. Find the right approach in our webinar with plasmidsaurus 📅 July 15 | 12 PM ET ✅ Sanger, short + long-read ✅ Interpret quality metrics ✅ Pick the right approach Register: https://twp.ai/4htXC1

💯💯 endorse - thank you @kamounlab.bsky.social and colleagues!

Sophien Kamoun@kamounlab.bsky.social · 4w ago

Just published: Community Spirit: Plasmids for All How a decade of plasmid-wrangling at @thesainsburylab.bsky.social grew into a movement — and the unexpected friends now helping us open the doors wider. medium.com/p/community-...

“the academic community is enormously heterogeneous and it’s almost impossible to keep everyone happy” Never a truer word said in this analysis of downstream consequences of openness by @jacoates.bsky.social ripplingideas.org/2026/06/26/t...

The 1% club and the rocky road ahead for bioRxiv

The recent launch of qed’s “top 1%” preprint ranking has sparked controversy, with concerns about its arbitrary nature, potential harm to authors, and low-quality inclusions. Whil…

ripplingideas.org

If you’ve ever wished for hundreds of immune-specific transcription factors in barcoded lentiviral vectors, ready to use in high-throughput combinatorial screens… that’s a very specific wish. But we’ve got you, thanks to a deposit by Pereira Lab Check it out!

blog.addgene.org

A 400-Plasmid Collection for Making Immune Cells from Scratch

twp.ai

Heading to Barcelona for #FENS2026? Come find us at Booth 147! We'd love to chat about your research and what's new at Addgene. See you there! 🧠 https://twp.ai/4hrfwD

My first art credit! 💅 Art director Samuel Velasco and I wanted a new visual metaphor for the genome — not a code or a sequence, but its tangled, twisted physical form. More cool yarn sculptures inside! Also it's a great piece by @philipcball.bsky.social that will change the way you think about DNA

Quanta Magazine@quantamagazine.org · 2mo ago

The human genome is no blueprint or algorithm — it’s something else entirely, something living with promiscuous logic. Can new genomic AI models, such as Evo 2 and AlphaGenome, untangle these tricky knots or will the genome confound them, too? www.quantamagazine.org/why-the-huma...

PHP.eB is now available via Addgene's Packaged on Request service. Not sure if it's the right capsid for your experiment? Our Viral Vectors 101 post on systemic capsids includes a decision tree and comparison table to help you choose.

blog.addgene.org

Viral Vectors 101: Systemic Capsids

twp.ai

This free webinar from my colleagues is tomorrow! Get an overview and intro to using #CRISPR in the lab - great for students or anybody new to the topic 🧬🧪💻 June 18, 12pm ET / 9am PT

Addgene@addgene.bsky.social · 2mo ago

At CRISPR 101 on June 18, 2026 you'll: ✅ Learn how CRISPR/Cas9 works ✅ Compare common CRISPR systems ✅ Identify the right system for your research Free, live, and presented by Addgene's own application scientists. Register today:

Excited to share our first red ACh sensor! 🔴 We developed GRAB_rACh1h, the first genetically encoded red fluorescent sensor for recording acetylcholine dynamics in vivo. (1/3)

😎 to have this out! --> stay tuned, this is a series about 📊#dataviz for #science 🧪

Nature Methods@natmethods.nature.com · 2mo ago

We are very excited to bring back our popular Points of View column, written by @helenajambor.bsky.social! Check out her first column in our July issue about the usage of color scales in scientific figures. www.nature.com/articles/s41...

🧪New from me for @nature.com: I do a lot of writing about technological innovation, but sometimes, the simpler tool really is best. For this story, I asked field and bench scientists to tell me about their favorite, most basic tools! www.nature.com/articles/d41...

In the field — and sometimes in the lab — the simplest tool is best

Technology has led to unprecedented innovation, but common household items can help make research more reproducible — and accessible.

nature.com

Neuroscientists, we’ll see you in Barcelona! 🧠Addgene is heading to #FENS2026 (July 6-10). Stop by Booth 147 during the expo (July 7-10) to chat about how we can support your research with plasmids, viral vectors, and more. Plus, enter our raffle for a chance to win Addgene swag! 🎉

At CRISPR 101 on June 18, 2026 you'll: ✅ Learn how CRISPR/Cas9 works ✅ Compare common CRISPR systems ✅ Identify the right system for your research Free, live, and presented by Addgene's own application scientists. Register today:

www.labroots.com

CRISPR 101: Getting Started with CRISPR

twp.ai

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

Has anyone observed mNeonGreen getting brighter after photobleaching?

PHP.eB — an AAV9 derivative known for efficient, brain-wide transduction via systemic delivery — is now available through Addgene's Packaged on Request service. What cargo do you need packaged? https://twp.ai/4hqr5l

Today I'm posting a new blog about an astonishing scientific own goal. Hundreds of papers have reported using a totally wrong antibody to investigate the tumor suppressor p16. This mistake has happened because scientists have muddled the names of two proteins. forbetterscience.com/2026/06/02/m...

In the latest issue of @jcellsci.bsky.social, with @lankylaste.bsky.social, Lina Gerontogianni and Gavin Kelly at @crick.ac.uk, a reanalysis of images from the IDR to answer the age-old question: How do you tell statistical significance from biological significance? doi.org/10.1242/jcs....

TL;DR: We've identified more than 100 cases of apparent manipulation in Thermo Fisher Scientific's antibody verification data. @sholtodavid.bsky.social @johanduchene.bsky.social reeserichardson.blog/2026/05/28/h...

How much of Thermo Fisher’s antibody data has been manipulated?

We’ve documented more than 100 instances of apparent data manipulation in Thermo’s catalog

reeserichardson.blog

Two popular CRISPR KO libraries just got easier to use! Jacquere and Julianna from the John Doench and Root labs are now available as ready-to-use lentiviral preps at Addgene. Human KO: https://twp.ai/9OVfvi Mouse KO: https://twp.ai/4hqQje

The use of antibodies in science is robust, but notoriously complicated due to a myriad of factors related to storage and implementation. This… this does not help. Do better Thermo Fisher.

Sholto David@sholtodavid.bsky.social · 3mo ago

Surprised to discover that Thermo Fisher appears to show a fake western blot for the validation of one of their p53 antibodies. I've added a diagram to show the very similar bands. This does not appear to be one of the "published figures" but their own internal data.

New plasmids from Kai Johnsson now online! 💡 The self-labeling CLIP-tag2 achieves almost 1000-fold faster tagging than the original when combined with a new substrate optimized for live-cell imaging. https://twp.ai/4hrKYy

Scientists shared their standout days for this @nature.com feature. Thx to @nicoleackermans.com @bwjones.bsky.social @aishikghosh.bsky.social @peixotoraquel.bsky.social & others for their stories! If you have a story, please send it my way for part 2!

Best. Day. Ever. What does a good day in science look like?

Struggling to remember why you picked a career in science? Here are some heart-warming tales to jog your memory.

nature.com

I’m not sure if I ever shared on Bluesky this article we wrote on how almost everyone giving career advice is susceptible to survivorship bias, but I think it was good and you should read it www.nature.com/articles/d41...

Beware survivorship bias in advice on science careers

For objective careers advice, talk to those who left science as well as those who stayed.

nature.com