α-Synuclein N-terminal acetylation tunes both its structural and physicochemical properties to enhance binding to lipid bilayers @ispt-proteinterm.bsky.social pubs.acs.org/bichaw/artic...

N-Terminal Amine Chemistry Influences α-Synuclein Interactions with Lipid Bilayers

Abstract. α-Synuclein (αSyn) is an intrinsically disordered protein whose reversible membrane binding via amphipathic α-helix formation is central to its f

pubs.acs.org

More on NAC and cotranslational Nt-acetylation by NatB. Congratulations to the first authors Natalia and Pawel! rdcu.be/ftSay

Structural basis of cotranslational protein N-terminal acetylation by NatB in human cells

Nature Communications - N-terminal acetylation is one of the most common protein modifications. Here, the authors show how NAC recruits the essential enzyme NatB to translating ribosomes, revealing...

rdcu.be

New preprint: In collaboration with @yorgsvicen.bsky.social we show metacaspase9 and ATE N-degron pathway-dependent proteoform stability act to diversify UBP6 function during plant immunity www.biorxiv.org/content/10.6...

Functional diversification of UBP6 in plant immunity through N‑degron pathway regulation

Deubiquitylases are key proteolytic regulators of ubiquitin-dependent cellular processes, catalyzing the removal or remodelling of ubiquitin modifications on substrate proteins, including those target...

biorxiv.org

After more than a decade of work, this amazing study is out in Cell 🧬 Global genetic interaction network of a human cell mapping conserved principles and informing functional interpretation of gene co-essentiality profiles 🔗 cell.com/cell/fulltext/S0092-8674(26)00345-4

Global genetic interaction network of a human cell maps conserved principles and informs functional interpretation of gene co-essentiality profiles

CRISPR perturbation of ∼4 million gene pairs in human HAP1 cells maps ∼89,000 genetic interactions, revealing a hierarchical network that links genes to complexes, pathways, and cellular processes and...

cell.com

Structural and mechanistic insights into the UBR4-KCMF1-Calmodulin complex @ispt-proteinterm.bsky.social academic.oup.com/proteincell/...

Structural and mechanistic insights into the UBR4-KCMF1-Calmodulin complex

Dear Editor,

academic.oup.com

📽️ G&D Tapes 📽️ G&D Author, Lucas Unger, tells us about an auto-regulatory loop between HNF1A and Hedgehog signaling that dictates stem cell lineage specification. New in #genesdev: ➡️ https://genesdev.cshlp.org/content/40/11-12/805.full Simona Chera & Cell Fate Lab‬ Lucas Unger Luiza Ghila

Structure of the E3 ligase CRL2ZYG11B with substrates reveals the molecular basis for N-degron recognition and ubiquitination. @ispt-proteinterm.bsky.social @cp-cellreports.bsky.social www.cell.com/cell-reports...

Structure of the E3 ligase CRL2ZYG11B with substrates reveals the molecular basis for N-degron recognition and ubiquitination

Liu et al. define how the CRL2ZYG11B ubiquitin ligase assembles and recognizes Gly/N-degron substrates by cryo-EM. Structures with an NLRP1 Gly/N-degron and SARS-CoV-2 ORF10 reveal extended substrate-...

cell.com

After a wonderful four day, @giglionelab.bsky.social wraps up #proteintermini2026 Grazie, Carmela for a fantastic meeting and we look forward to Heidelberg in 2028!

Well, the conference dinner is absolutely hellish #proteintermini2026

#Proteintermini2026 is about to begin! This beautiful room in the heart of Palermo will be our home for the next four days 🤩

And we’re off! @giglionelab.bsky.social introduces FEBS Workshop #Proteintermini2026 Looking forward to four days of great science

As predicted, the food at #Proteintermini2026 is delicioso! Trying hard to resist these ricotta-filled pastries with candied orange

1/ Sharing my article in TIBS: "Science is always unfinished: rethinking the demand for complete stories." The cost of that demand: delayed dissemination, cherry-picked data, and an endless "one more experiment" loop. authors.elsevier.com/a/1n0XU3S6Gf...

The countdown to Palermo is on! 🇮🇹 The FEBS Workshop "Protein Termini 2026" is set. • 151 participants from all over the world. • >50% ECRs! • 30+ travel grants. Thx to sponsors: FEBS, IUBMB, CDBC, ARS, and others.. See you at Palazzo dei Normanni! #ProteinTermini #FEBS #Proteostasis

Excited to co-organize the upcoming FASEB Science Research Conference (SRC) on Protein Lipidation: Enzymology, Signaling, Therapeutics! July 13-15, 2026. Where: Yours Truly DC Hotel | Washington, DC. Register & Submit your abstract here: events.faseb.org/event/protei... Deadline Extended!

New review out on protein N-terminal formylation - a stress-inducible modification of eukaryotic proteins #N-degron @ispt-proteinterm.bsky.social www.nature.com/articles/s12...

N-terminal formylmethionine as a degron and a specific signal in proteostasis and stress adaptation - Experimental & Molecular Medicine

N-terminal methionine formylation, a protein modification once thought to be exclusive to bacteria and organelles, also occurs on proteins synthesized in the cytosol of yeast and human cells, challeng...

nature.com

It's finally out! With this new paper, Salma and Monica show that hypoxia-regulated ERFVIIs are not immediately destabilised during reoxygenation: they instead persist in the nucleus to control oxidative stress genes. www.nature.com/articles/s41...

N-terminal cysteine dependent proteolysis of RGS4/5 is not sensitive to physiological oxidative stress | Preprint by Ya-Min Tian, Haeun Kim, Peter Ratcliffe & Thomas Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron #cysteine www.biorxiv.org/content/10.6...

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation www.pnas.org/doi/10.1073/...

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation | PNAS

The Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...

pnas.org

Resistance to prolonged darkness in plants steered by N-terminal acetylation | New study by Gong et al. Markus Wirtz lab @giglionelab.bsky.social Tanja Bange lab #NatB #autophagy #N-degron #proteostasis www.nature.com/articles/s41...

The ribosome-associated N-terminal acetyltransferase B coordinates global proteostasis and autophagy in plants by creating Ac/N-degrons - Nature Communications

Protein N-terminal acetylation profoundly influences protein fate. Here, the authors establish NatB as a central regulator of the interplay between ubiquitin–proteasome system and autophagy, and highl...

nature.com

Online Now: NAC promotes co-translational protein folding at the ribosomal tunnel exit Online now:

NAC promotes co-translational protein folding at the ribosomal tunnel exit

Santos et al. reveal NAC’s co-translational interactome, showing compartment-specific nascent chain interactions in human cells. NAC engages emerging chains at the ribosomal tunnel exit via a ribosome-oriented hydrophobic pocket, promoting early domain folding without stabilizing intermediate structures. These findings establish NAC as a co-translational chaperone integrating folding with protein biogenesis.

dlvr.it

Congratulations Jake @mediocre-jake.bsky.social, Bekky Feltham and team on their phenomenal effort in putting together the E3-ome, published in Cell www.cell.com/cell/fulltex.... An extremely valuable resource for ubiquitin researchers.

The E3-ome gene-centric compendium reveals the human E3 ligase landscape

The E3-ome defines the human repertoire of ubiquitin E3 ligases, creating a unified resource that maps their diversity across the ubiquitin and ubiquitin-like systems. By consolidating fragmented know...

cell.com

WEHI (The Walter and Eliza Hall Institute of Medical Research)@wehi-research.bsky.social · 5mo ago

WEHI researchers have led a major global effort to create the first authoritative atlas for E3 ligases, resolving more than 18 years of inconsistencies in the ubiquitin field. Study led by Dr Ngee Kiat ‘Jake’ Chua & Dr Rebecca Feltham in @cellpress.bsky.social www.wehi.edu.au/news/new-enz...

How are histones acetylated as they emerge from the ribosome? We show that NAA40 cooperates with the NAC complex to enable co-translational acetylation of H2A/H4, revealing NAC as a coordinator of nascent protein modification. Read more about our collaboration with @kirmizislab.bsky.social #cryoEM

NAA40 and NAC cooperate in co-translational histone acetylation in humans

Nature Communications - N-terminal histone acetylation by human NAA40 alters epigenetic signaling and affects gene regulation. Here, the authors combine biochemistry and cryo-EM to unravel the...

rdcu.be

Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry: Cell Systems www.cell.com/cell-systems.... How great would it be to sequence proteins without a gene-template! We might be approaching this soon.

Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry

Highly efficient hyperthermal acidic proteases combined with hybrid-fragmentation schemes provide five times more unique peptide reads than trypsin or chymotrypsin, greatly boosting confidence in de n...

cell.com

A wonderful collection of contemporary topics and perspectives on secretion and Golgi biology edited by Akihiko Nakano and Jaakko Saraste. The Golgi Network, Volume II @SN and link.springer.com/book/10.1007...

The Golgi Network, Volume II

This book explores the interaction of Golgi with different organelles such as ER, the vesicular system, and protein complexes that execute these tasks.

link.springer.com

My review “Co-translational control of protein stability and quality in plants” is now online at @jxbotany.bsky.social, in which I describe how co-translational processing and ribosome-associated quality control together establish protein stability and fate early in synthesis. tinyurl.com/5dhhz9h6

Co-translational control of protein stability and quality in plants

Abstract. Proteostasis relies on the coordinated control of protein synthesis, folding, modification and degradation, and an increasingly clear picture is

tinyurl.com

8 days left to register | 28 invited speakers | Keynotes from F. Ulrich Hartl, Roland Beckmann and Michael Rapé | #chaperones #degradation #ubiquitin #cryoEM #acetylation #lipidation #ribosomes #proteins proteintermini.org/meeting/

Actin N-terminal maturation can be blocked by a butynamide stereoprobe which acts as an ACTMAP inhibitor. Preprint by Xiong et al. Benjamin Cravatt and Bruno Melillo. @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.6...

Join us in Palermo! FEBS Workshop Protein Termini 2026: the power of protein termini across bacteria, plants, and animals—from ribosome biology to proteostasis and applications. Deadline: 3 March 2026 proteintermini.org/meeting #ProteinTermini #Proteostasis #FEBS #EMBO @iubmb.bsky.social

Join us at the @crick.ac.uk for the 2026 meeting of the UK proteostasis community! We especially encourage students and postdocs to attend and share their work. All talks (except the keynotes) will be selected from abstracts.

Proteostasis UK@proteostasisuk.bsky.social · 6mo ago

📣 UK Proteostasis Meeting 2026 – Registration Now Open! I’m delighted to share that registration is now open for the UK Proteostasis Meeting 2026, hosted by The Francis Crick Institute on 20–21 July 2026
. Please register here(lnkd.in/ervXMzWN) and through Eventbrite for payment (lnkd.in/eTxqjnQy)