Gina Simon, Doris Hellerschmied @hellerschmiedlab.bsky.social and colleagues @unidue-zmb.bsky.social dissect stress-induced proteome remodelling at the Golgi–endosome interface. Highlight: journals.biologists.com/jcs/article/... Article: journals.biologists.com/jcs/article/...

If you are interested in how single cells are able to learn, check out this beautiful article by @niko-kukushkin.bsky.social www.sciencedirect.com/science/arti...

Cellular memory: Learning without building

A new study reveals that habituation in the single-celled ciliate Stentor coeruleus uses the same calcium and kinase signaling as animal memory but do…

sciencedirect.com

Thank you to all the friends, collaborators, lab members and mentors who made this happen!

As we are sending back the revised version of our pooled prime editing gene tagging paper, I am happy to share a website for design of prime tagging libraries, including some images, all generated from a single pooled transduction and in situ sequencing. primetagging.org

Very happy to share our recent preprint describing #Golgi protein quality control #PQC in mammalian cells. #proteasome #ubiquitin #ChemBio Fantastic work by @joaodiamantino.bsky.social and team! #proudPI @unidue-zmb.bsky.social @imprs-lm.bsky.social

bioRxiv Cell Biology@biorxiv-cellbio.bsky.social · 6mo ago

Selective proteasomal degradation from the Golgi apparatus membrane https://www.biorxiv.org/content/10.64898/2026.02.04.703791v1

The latest preprint from our lab! Lipid droplets promote the aberrant liquid-liquid phase separation of alpha-synuclein leading to impaired energy homeostasis www.biorxiv.org/content/10.1...

Lipid droplets promote the aberrant liquid-liquid phase separation of alpha-synuclein leading to impaired energy homeostasis

Alpha-synuclein (αSyn) inclusions, termed Lewy bodies, are the characteristic neuropathological feature of Parkinson's disease. Growing evidence points towards a role of aberrant liquid-liquid phase s...

biorxiv.org

www.oncologypipeline.com/apexonco/tri...

Triple meeting 2025 – Halda’s proximity play in prostate

Early data with the RIPTAC HLD-0915 look competitive.

oncologypipeline.com

Very happy to be in Nashville today for #zebrafishzunday before my talk @vanderbilt.edu tomorrow, so here's a sneak preview of differential clearance of monomeric (turquoise) vs. oligomeric (red) amyloid beta from the zebrafish brain

Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...

Cells can form patterns within themselves just like embryos do. How? Connie Yan's new preprint shows how the anterior-posterior cytoskeleton pattern in Stentor is dictated by regionalized scaffolding proteins www.biorxiv.org/content/10.1...

Be my neighbor! @rwjms.bsky.social Neuroscience is hiring! jobs.rutgers.edu/postings/258...

Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩

STAR Protocols@cp-starprotocols.bsky.social · 12mo ago

Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9 #protocol #starprotocols #cellpress

Very happy to see our contribution to a better understanding of Hsp70/90 #chaperones online! Congrats and a big thank you to all co-authors, collaborators and reviewers! #proteostasis @poepsel-lab.bsky.social @crc1430.bsky.social @unidue-zmb.bsky.social

Nature Structural & Molecular Biology@natsmb.nature.com · last yr.

New online: Conformational plasticity of a BiP–GRP94 chaperone complex

Excited to share our lab’s first publication! A review on how pooled protein tagging with ligandable domains is unlocking proteome-scale biology. Spearheaded by the talented @kelarrimore.bsky.social. www.frontiersin.org/articles/10....

Frontiers | Utilizing small molecules to probe and harness the proteome by pooled protein tagging with ligandable domains

Multifunctional ligand-binding domains have revolutionized cell biology by enabling the precise visualization and manipulation of fused proteins of interest ...

frontiersin.org

So excited to share that I am one of the new @hhmi.org freeman hrabowsi scholars with so many other amazing new PIs. Check out the full list here www.hhmi.org/programs/fre...

The 2025 Freeman Hrabowski Scholars | HHMI

Freeman Hrabowski Scholars are outstanding early career faculty who have the potential to become leaders in their research fields.

hhmi.org

Our ‘Cell Scientist to Watch’ is Francesca Bottanelli @franbottanelli.bsky.social‬. We discover more about Fran’s career path, her approach to scientific mentorship and her dream to start a band! journals.biologists.com/jcs/article/...

Excited to see this out in @science.org today!! www.science.org/doi/10.1126/...

Intracellular protein editing enables incorporation of noncanonical residues in endogenous proteins

The ability to study proteins in their native cellular context is crucial to our understanding of biology. In this work, we report a technology for intracellular protein editing, drawing from split in...

science.org

George Burslem@gburslem.bsky.social · 2y ago

Ever wanted to edit a protein sequence inside a living mammalian cell? Maybe to site specifically incorporate a non-canonical residue? Perhaps you’d like to do a pull down without an antibody? Or use a small molecule fluorophore to image an endogenous protein? 1/4

Excited to to see our collaboration with @gburslem.bsky.social published today! This is part of our ongoing long term goal of achieving scalable non-disruptive and direct imaging and perturbation of the endogenous proteome

George Burslem@gburslem.bsky.social · last yr.

Excited to see this out in @science.org today!! www.science.org/doi/10.1126/...

Thrilled to see our work now in @cp-molcell.bsky.social! It was an incredible privilege to do my postdoc with @ophirshalem.bsky.social and work alongside the super talented Steph Sansbury and our amazing co-authors. Excited to keep building on this research in our new lab at @rwjms.bsky.social!

Ophir Shalem@ophirshalem.bsky.social · last yr.

Check out our paper, now extensively revised and published in @cp-molcell.bsky.social! This has been a real tour de force led by Stephanie Sansbury and @ysereb.bsky.social and we are very excited about the findings and future potential of this approach www.cell.com/molecular-ce...

Bsky vs Twitter engagement difference now 50-fold...

#StandUpForScience2025

New paper online today! Check out @cewc9.bsky.socials review on how acetylation mediated protein degradation impacts cancer! Thanks for the invite to contribute to the 10th anniversary special issue of Trends in Cancer! www.sciencedirect.com/science/arti...

Acetylation: a new target for protein degradation in cancer

Acetylation is an increasing area of focus for cancer research as it is closely related to a variety of cellular processes through modulation of histo…

sciencedirect.com

We have a postdoc position available for a very cool collaborative project! Synthetic chemistry expertise is a must! Unfortunately due to the funding source, this position is only available to US citizens or permanent residents. chemistryjobs.acs.org/job/postdoct...

I am extremely proud to see our work about Hepatitis B now published in @cellpress.bsky.social From basic research to identifying the key step of infection & a potential first drug for curing this devastating disease. This has been an incredible journey.🧵 www.cell.com/cell/fulltex...

A nucleosome switch primes hepatitis B virus infection

A reconstituted cccDNA platform facilitated the discovery of an early chromatinization event that promotes transcription of the hepatitis B virus (HBV) regulatory X gene. The use of a small-molecule c...

cell.com

Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! arxiv.org/abs/2502.12675 1/n

Fast Antibiotic resistance-Based gene editing of mammalian cells with CRISPR-Cas9 (FAB-CRISPR)

Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency causing low knock-in rates. We ...

arxiv.org

1/n Excited to share our new preprint! Using live imaging in Drosophila embryos, we show that RNA Pol II clusters switch from sites of initiation to elongation during zygotic genome activation and that they are stably associated with an active gene: www.biorxiv.org/content/10.1...

The Trump NIH is banning hires of new students and postdocs. This is devastating for NIH research and will result in a lost generation of researchers at the institution. Heard from a applicant today: "A lot of people are banking their entire careers on the [NIH student programs]..." #NIH 🧪 1/

The Serebrenik Lab is hiring! Join us in unraveling proteostasis with pooled protein tagging and manipulation! Interested postdocs can learn more & meet other excellent labs at the Rutgers Postdoc Peek (Apr 3-4, 2025). Apply by 1/8/25: rutgers.ca1.qualtrics.com/jfe/form/SV_...

Single cells can learn without a brain, but how? Our new preprint from @deeparajan.bsky.social describes a model for habituation in Stentor coeruleus, based on receptor internalization and recycling, along with new experiments inspired by the model. 1/n www.biorxiv.org/content/10.1...

Happy to share our new paper where we explore why dCas9-KRAB protein levels drop so drastically after neuronal differentiation in WTC11 and KOL2.1J iPSC lines. www.biorxiv.org/content/10.1...

CRISPR associated enzymes are mislocalized to the cytoplasm in iPSC-derived neurons resulting in KRAB-specific degradation

The use of CRISPR-associated enzymes in iPSC-derived neurons for precise gene targeting and high-throughput gene perturbation screens offers great potential but presents unique challenges compared to ...

biorxiv.org