Dan Grabarczyk
@dangrabarczyk.bsky.social
Structural biologist - currently ubiquitin ligases, previously DNA replication and bacterial metabolism Research Associate - Clausen Lab, IMP Vienna
Excited to share my paper! 🥳 Today in #Nature we reveal an 8 MDa Hdr electron bifurcating super-assembly in class I methanogens that couples the first and last steps of hydrogenotrophic methanogenesis. A lineage-specific adaptation to anaerobic niches. www.nature.com/articles/s41...
1/ Happy to share our preprint! The Mtr complex catalyses the second-to-last step of the methanogenesis and is an essential bioenergetic machine in most methanogenic archaea. Anaerobic cryo-EM of Mtr reveals a nitrogenase-like metallocluster bound at its active site! www.biorxiv.org/content/10.6...
Super excited to launch this search for a new endowed professor at the @biozentrum.unibas.ch! Join us to build a research cluster in Climate Biophysics! 🌍🌊🌾🔬🧪 This is a unique opportunity to create a community in Basel🇨🇭working together on this timely topic! ⏳🌡️ Questions? Please reach out! 👩🔬👨🔬
Biozentrum, University of Basel@biozentrum.unibas.ch · last mo.New Assistant (tenure-track) or Associate Professor position in Climate #Biophysics! Join our international research community to explore fundamental molecular mechanisms underlying climate-relevant biological processes. Apply by 7 Sept 2026! www.biozentrum.unibas.ch/de/open-posi...
Membrane protein solubilization and structure determination using de novo–designed proteins | Science www.science.org/doi/10.1126/...
Membrane protein solubilization and structure determination using de novo–designed proteins
Developing therapies and vaccines against integral membrane proteins is hindered by their extensive hydrophobic surfaces, which complicate production and structural analysis. Here, we describe a gener...
science.org
1/7 New preprint: we found that Chlamydomonas carries a giant and unexpectedly elaborate chloroplast RNA polymerase with 12 previously unknown subunits, overturning the idea that the complexity of this organellar enzyme emerged during plant terrestrialization! www.biorxiv.org/content/10.6...
The structure of a 2-MDa chloroplast RNA polymerase reveals unexpected evolutionary complexity
Transcription in chloroplasts depends on the Plastid-Encoded RNA polymerase (PEP), a bacterial-derived enzyme whose catalytic core remains encoded by the highly reduced genome inherited from the cyano...
biorxiv.org
Structural and mechanistic insights into the UBR4-KCMF1-Calmodulin complex @ispt-proteinterm.bsky.social academic.oup.com/proteincell/...
Structural and mechanistic insights into the UBR4-KCMF1-Calmodulin complex
Dear Editor,
academic.oup.com
🔍 Sample heterogeneity is both a challenge and an opportunity in #cryoEM data processing. In our latest #CryoSPARC case study we explore EMPIAR-10927, a dataset with acetogenin-bound mitochondrial Complex I, that reveals many more complexes according to the data processing strategy 👉 bit.ly/4uihh8V
Case Study: Discrete heterogeneity in a sample of Acetogenin-bound complex I (EMPIAR 10927) | CryoSPARC Guide
Processing EMPIAR-10927 including separating targets using Per-particle scales or custom settings in Ab-Initio Reconstruction, and using 3DVA to guide sub-classification choices.
bit.ly
New preprint. In S. sanguinis, a C5a peptidase-like protease (ScpH) polymerizes along eDNA, turning it into a catalytic biofilm scaffold. Great work from @robinanger.bsky.social in our lab, with Pelicic lab @lcbofficiel.bsky.social www.biorxiv.org/content/10.6...
Open Postdoc Position - Giant E3 ligases: mysterious mechanisms & non‑protein substrates. Interested? Join us to uncover how these molecular giants tag lipids, RNAs, sugars beyond proteins, on organelles and pathogens, to defend the cell. Details & Application here www.imp.ac.at/career/open-...
Preprint with new server~ Using in situ cryo-ET, we identify a previously uncharacterized higher-order architecture of urate oxidase in mouse liver. langtaosha.org.cn/lts/en/prepr...
A conserved helix-tiling architecture of urate oxidase organizes metabolism in peroxisomes
Cells organize metabolism within a crowded intracellular milieu, yet the structural logic and functional consequences of supramolecular enzyme assemblies in vivo remain poorly defined. Here, using in
langtaosha.org.cn
Excited to share our work on the structure and function of cytoplasmic lattices within mouse embryos. A collaborative effort with @niakanlab.bsky.social and work led by @kashishsingh.bsky.social and @inaharasimov.bsky.social . It is now out on BioRxiv: www.biorxiv.org/content/10.6...
Proper organelle regulation during mitosis is a must, but how do membrane-less condensates like PML bodies behave during cell division? In work led by Eric Aird, we found the balance of speckled proteins SP110 & SP100 to be the key. doi.org/10.1038/s415...
Registration now open for #EMBOUbiquitin. meetings.embo.org/event/26-ubi... Childcare, accessibility, and travel grants available. #ubiquitin #TargetedProteinDegradation #Proteostasis #UBL
Ubiquitin and ubiquitin-like proteins in health and disease
The ubiquitin system is fundamental to virtually all biological processes and is essential for maintaining cellular and organismal homeostasis. Recent technological advances are revealing new roles f…
meetings.embo.org
Rachel O'Dea@rnidhea.bsky.social · 8mo agoSAVE THE DATE! The EMBO workshop "Ubiquitin and Ubiquitin-like proteins in Health and Disease" will be on September 2026. Exciting line up of talks and an amazing location! Organised by @kulathu.bsky.social, @merbllab.bsky.social, @simonapolo.bsky.social, Claudio Joazeiro. Registration open soon.
We are excited to be recruiting a new tenure track group leader in the Structural Studies Division at MRC LMB! It is an amazing place to start your own lab. @mrclmb.bsky.social Please get in touch if you have any questions. www.nature.com/naturecareer...
Research Group Leader Tenure Track - Structural Studies - LMB 2775 - Cambridge, Cambridgeshire (GB) job with MRC Laboratory of Molecular Biology (LMB) | 12854636
Research Group Leader Tenure Track Starting Salary £67,979 to £76,868 per annum MRC Laboratory of Molecular Biology, Cambridge, UK The MRC Laborato...
nature.com
Sjors Scheres@sjorsscheres.bsky.social · 6mo agoPlease spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳 mrc.tal.net/vx/mobile-0/...
The new ChimeraX CubeNTube plugin allows erasing parts of maps using cube, cylinder, and custom shapes and has undo. Created by Tamino Cairoli. Available from ChimeraX menu Tools / More Tools....
Our paper is out in @natcomms.nature.com! APOBEC3s drive mutagenesis in cancer. We uncover a novel pathway keeping them in check. The key is APOBEC3 binding of cellular RNAs, which simultaneously controls their nuclear localization and shields them from degradation. Read it here: rdcu.be/e4qaa
To regulate gene activity, proteasomes - waste disposal machines of cells - must enter the nucleus. IMP Researchers now show how the adaptor protein AKIRIN2 helps ferry this massive complex through the nuclear pore: https://www.nature.com/articles/s41467-026-69162-0
Some cool E3 ligase structures here with calmodulin-mediated oligomerisation. Also interesting collection/processing to deal with preferred orientation. www.biorxiv.org/content/10.6...
biorxiv.org
@lorenzoorts.bsky.social previously worked as a postdoctoral fellow at @impvienna.bsky.social in Austria. She will establish a new lab at IMB to investigate how mRNA translation is activated in the early embryo. Welcome to IMB Laura! 💐 Read more here: www.imb.de/about-imb/ne...
After a long (but fun!) postdoc journey, I am very excited to share that I will start my own lab in the Ubiquitin Signalling Division at WEHI. We will study how ubiquitin controls mitochondrial health and its role in neurodegenerative disease. Hiring soon!
WEHI (The Walter and Eliza Hall Institute of Medical Research)@wehi-research.bsky.social · 6mo agoSay hello to our incoming lab heads! 👋 Excited to announce Dr Danika Hill, Dr Jacki Heraud-Farlow and Dr Sylvie Callegari will soon be establishing new labs at WEHI. More info: www.wehi.edu.au/news/excepti... bsky.app/profile/sylv... bsky.app/profile/jack... bsky.app/profile/prec...
When RNA Degradation 🤝 meets 🤝 Protein Degradation! tinyurl.com/E3TDMD In a collaboration of @bartellab.bsky.social and Schulman lab, we show that, in target-directed microRNA degradation (TDMD), 2-RNA-factors recruit an E3 ligase and induce the degradation of not only a protein but also RNA (1/5).
Vaults. They are cell biology's greatest puzzle! This preprint from Martin Beck's lab shows them docked on ER membranes with a ribosome inside. What on earth is going on there?? #CellBiology #WTFology www.biorxiv.org/content/10.6...
The vault associates with membranes in situ
The eukaryotic vault particle is a giant ribonucleoprotein complex that assembles into an iconic barrel-like cage. Its cellular function has remained elusive despite extensive characterization. Using ...
biorxiv.org
SAVE THE DATE! The EMBO workshop "Ubiquitin and Ubiquitin-like proteins in Health and Disease" will be on September 2026. Exciting line up of talks and an amazing location! Organised by @kulathu.bsky.social, @merbllab.bsky.social, @simonapolo.bsky.social, Claudio Joazeiro. Registration open soon.
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
Filament formation and NAD processing by noncanonical human FAM118 sirtuins
Nature Structural & Molecular Biology - Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that...
rdcu.be
A new Review discusses how degrons of different types, with an emphasis on high-order structural ones, regulate substrate-E3 interactions and how their dysregulation is linked to human diseases www.nature.com/articles/s41...
Degrons and degradation signals beyond short linear motifs - Nature Chemical Biology
Degrons are degradation signals in target proteins to direct recognition and degradation by the ubiquitin–proteasome system. Wang et al. introduce degrons of different types, with an emphasis on high-...
nature.com
An Asgard archaeon with internal membrane compartments Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors! www.biorxiv.org/content/10.1...
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
Split-site ubiquitination gives ZNFX1 new power in RNA defense @cellcellpress.bsky.social www.cell.com/molecular-ce... www.sciencedirect.com/science/arti...
Split-site ubiquitination gives ZNFX1 new power in RNA defense
Recent work by Grabarczyk et al. uncovers the molecular mechanism by which ZNFX1, an interferon-stimulated gene, employs a novel split-site E3 ligase domain structure to ubiquitinate both protein lysi...
cell.com
Elaborate machinery for inserting proteins into the outer membrane of bacteria www.nature.com/articles/d41...
Elaborate machinery for inserting proteins into the outer membrane of bacteria
Bacteria in the gut use an unusual molecular apparatus to insert complex proteins into their outer membrane.
nature.com
