New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚

Oh wow!

Yamuna Krishnan@krishnanyamuna.bsky.social · 2mo ago

1/ Mitochondrial Na⁺ links metabolism, Ca2+ signaling and disease. We know how it gets in, how it gets out, and proteins that use it. But: we've never actually seen Na⁺ in single mitochondria in live cells. We started with an unusual observation, went down a rabbit hole & found some cool biology .

The best place for biophysics!

Cees Dekker@ceesdekker.bsky.social · 2mo ago

WORK: our Bionanoscience department at TU Delft is looking to hire an Assistant Professor in Biophysics of Living Systems. Apply! Deadline 30 July careers.tudelft.nl/job/Delft-As... RT=nice

Bacteria don't need signaling cascades to rewire gene silencing. They use DNA 🧬 twist. ➕Overwind → H-NS forms a filament. ➖Underwind → H-NS bridges and silences. 📣Topology is the switch. See our preprint for more details: doi.org/10.64898/202...

bioRxiv Biophysics@biorxiv-biophys.bsky.social · 3mo ago

A DNA-binding protein senses DNA superhelicity to switch between bridging and nucleoprotein filament formation https://www.biorxiv.org/content/10.64898/2026.05.23.727375v1

Is gene regulation only dictated by biochemistry, or are we missing the bigger physical picture? 🧬✍️ Find our latest review article with Sarah A. Harris and Wilma K. Olson in Current Opinion in Structural Biology, where we advocate for "Physical Genomics” doi.org/10.1016/j.sb...

A new high-resolution microscopy technique enables simultaneous visualization of up to 12 biomolecules in cancer cell nuclei, offering detailed insights into nanoscale nuclear organization. doi.org/hbzd5k

High-resolution imaging shines light on nanoscale nuclear organization

Researchers at the Indian Institute of Science (IISc) have implemented an advanced microscopy technique to visualize multiple biomolecules inside the nucleus of a cancer cell simultaneously at incredibly high resolution.

phys.org

Our latest paper is online. www.nature.com/articles/s41... We expanded the speed-optimized DNA-PAINT sequences from 6 to 12 — enabling faster, higher-resolution multiplexed super-resolution imaging of up to 10 cellular targets. Here's the story. 1/n

High-speed multiplexed DNA-PAINT imaging of nuclear organization using an expanded sequence repertoire - Nature Communications

Banerjee, Anand, and colleagues present an expanded speed-optimized DNA-PAINT sequence repertoire. This repertoire allows for improved multiplexing capability in super-resolution imaging, maintaining ...

nature.com

Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort. www.biorxiv.org/cgi/content/...

Happy to share our Current Opinion review on the key challenges in accurately predicting 3D distances between chromatin segments and computing their dynamics. Thanks to @djost-physbiol.bsky.social @lucagiorgetti.bsky.social for the invitation to write this review! @shuvadipdutta.bsky.social

Thanks for highlighting our work on combining AI-based organelle dynamics detection in phase contrast with fluorescence! www.nature.com/ncomms/edito... @natcomms.nature.com and Nelio Rodriguez. Spearheaded by @wl-stepp.bsky.social with collaborators @jclandoni.bsky.social @maweigert.bsky.social

How short can a degron be? A compact, genetically encoded, 9-aa tunable degron that drives MDM2-dependent degradation enabling precise control of protein levels from yeast to human cells. Thanks to @eswarappalab.bsky.social for the opportunity to explore this in yeast. #Degron #Yeast #CellBiology 🧬

Eswarappa Lab IISc@eswarappalab.bsky.social · 6mo ago

Interested in tunable protein degradation? Check out our new preprint describing a compact, portable degron derived from MTCH2: doi.org/10.64898/202... Led by Lekha EM Saubhik Som Ramon Boro Sandeep Eswarappa Great collaboration w/ Anubhav Dhar Eepsitha @syncellbiolab.bsky.social #iiscbangalore

Interested in tunable protein degradation? Check out our new preprint describing a compact, portable degron derived from MTCH2: doi.org/10.64898/202... Led by Lekha EM Saubhik Som Ramon Boro Sandeep Eswarappa Great collaboration w/ Anubhav Dhar Eepsitha @syncellbiolab.bsky.social #iiscbangalore

I’m honored to be part of the next GRS Single Molecule Approaches to Biology, thanks Chairs @qmsmith.bsky.social and Alba Gomez Segalas for organizing this great event! The GRS is a wonderful opportunity for young scientists to share their exciting science and build communities. See you there!

Quentin Smith@qmsmith.bsky.social · 7mo ago

🗣️ Keynote Speaker: @sulianamanley.bsky.social 👥 Mentoring Session: Careers Across Biophysics featuring @valdavinson.bsky.social @timcraggs.bsky.social @sulianamanley.bsky.social @esisamakis.bsky.social 📈 Selected talk and poster presenters: You! ☀️ Please repost, share and we'll see you in June!

We developed a 'smart STED microscope' to detect rare objects/events in cells, and unraveled the 3D morphology of a nuclear membraneless organelle (paraspeckle). Amazing collaboration with Sjoerd Stallinga, Bernd Rieger (TU Delft) and @mixmue.bsky.social 🙏 (1/3) www.nature.com/articles/s41...

Excited to share a new preprint from a team of researchers I am part of (funded by an NIH grant). The paper helps us understand a question I have been interested in for a while: why are bacteria so resilient? www.biorxiv.org/content/10.1... @meyerroc.bsky.social @juliebiteenlab.bsky.social

(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen! Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org! doi.org/10.1073/pnas... 👇🧵

Thank you for having me here, Mike. Big thanks to all the organizers. It was fantastic meeting. Looking forward to the next editions of #SMLMS2025

Heilemann Lab@heilemannlab.bsky.social · 12mo ago

Next on stage is Mahipal Ganji from the IIT Bangalore @ganjilab.bsky.social shares great work on stacking interactions in DNA/RNA and multplexed-DNA PAINT to measure nuclear ultrastructure #SMLMS2025

📢 Dear lovers of prokaryotic chromosomes: Registration for the Biology & Physics of Chromosomes meeting has been extended until 7 August ⏳. Join us! @biophychrom.bsky.social @danielabarilla.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social #microsky

Talk slots full but we can take late breaking poster abstracts until August 7th. Hope to see you in York to hear about your chromosome story 🧬🦠🧫 www.eventsforce.net/biochemsoc/f... @vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social @biophychrom.bsky.social

And the story is published now!🍾 www.nature.com/articles/s41... Open access link: rdcu.be/exnOc Many years in the making, great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social Thanks @ukri.org BBSRC & @leverhulme.ac.uk for funding, reviewers & editor!! 🙏 #microsky #archaeasky

Coupling chromosome organization to genome segregation in Archaea - Nature Communications

Proteins SegA and SegB are important for chromosome segregation and organization in archaea of the order Sulfolobales, but mechanisms are unclear. Here, Kabli et al. uncover patterns and mechanisms th...

nature.com

Daniela Barillà@danielabarilla.bsky.social · 2y ago

New preprint from our lab! Story started long ago that took many years in the making. Great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social groups. Generously funded by BBSRC @ukri.org & @leverhulme.bsky.social How do #archaea segregate their chromosome? #microsky #archaeasky

Very much looking forward for #SMLMS2025

🔬 Are you working with single-molecule super-resolution methods (SMLM, MINFLUX) and would like to meet experts in the field and discuss your work? 👉🏻 consider attending the SMLMS 2025 Meeting in Bonn 👉🏻 abstract submission is open until June 27th 👉🏻 www.smlms.org @smlm-symposium.bsky.social

Such fantastic news! I had the privilege of working with Cees — an incredibly inspiring scientist. His research truly deserves this recognition. Huge congratulations, Cees!

Cees Dekker@ceesdekker.bsky.social · last yr.

Wow, I feel greatly honored by being awarded the 2026 Biochemical Society Award for Significant Breakthrough or Achievement, as was just announced! www.biochemistry.org/about-us/new... It is wonderful that the Biochemical Society honors our contributions to single-molecule biophysics in this way!

Latest #CDlab paper online in @labonachip.rsc.org: pubs.rsc.org/en/content/a... Here, 1st authors Alex Joesaar & Martin Holub developed a microfluidics platform to extract and study full length megabase-size genomes in microfluidic chambers (where they are protected against shearing by flow)!

Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?

In @molecularmicro.bsky.social: two special issues on Prokaryotic Chromosome Organization. Many excellent contributions, including a vision article: 'Future directions of the Prokaryotic Chromosome Field', outcome of BioPhyChrom 2023 @biophychrom.bsky.social onlinelibrary.wiley.com/doi/10.1111/...

Future Directions of the Prokaryotic Chromosome Field

In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of ....

onlinelibrary.wiley.com

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

The kindest of gentle reminders. We have one week to go on one #PhDPosition and one #PostdocPosition in single-molecule dielectrophoresis. Do you like single molecules , advanced optics and nanotechnology? More details in the thread👇 Dear Bluesky poplulation, please help with reposting

Sergii Pud@sergiipud.bsky.social · 2y ago

Hi all, I am very excited to share two positions (PhD and PostDoc) in my group on a EU-funded project ProAct. More about the positions below. Please share with your colleagues! This will be a great project to work on and also we offer a great environment at the BIOS group

Thermodynamic principles link in vitro transcription factor affinities to single-molecule chromatin states in cells www.biorxiv.org/content/10.1... ▶️40-fold flanking sequence effect on TF binding ▶️Motif recognition beats kinetics ▶️Minutes-long residence times ▶️Models of TF-nucleosome competiton

This is a fantastic community to be part of. Don’t miss the chance!

Grainger Lab@graingerlab.bsky.social · 2y ago

If you're interested in chromosome biology (DNA folding, gene regulation, replication, polymer modelling and more) we're organising the below this autumn. It'd be great to see you there. Please share if you can. :-)