Our new preprint @biorxivpreprint.bsky.social, led by @katsuminami.bsky.social‬ et al.! 🎉 www.biorxiv.org/content/10.6... Machine learning-assisted Repli-Histo labeling and single-nucleosome imaging reveal distinct transcription-dependent chromatin constraints across euchromatin and heterochromatin.

The replication-risk sequence is an essential bacterial DNA sequence linked to DNA gap formation. For the first time, we directly observed this gap formation at single-molecule resolution! We show that the gaps occur exclusively on the lagging strand and reveal why. www.biorxiv.org/cgi/content/...

1/ Out today in Science (@science.org): a result a decade in the making, and one that changed how I think about what cells are doing when they grow. We found a bacterium that grows slower than it could. And we found the molecular switch that lets us reverse it.

If you have experience in biochemistry/cryo-EM, are interested in DNA replication, and would like to do a postdoc in New York - our lab has positions available! Just DM or email me with your CV.

Last (?) preprint as a postdoc in the Merrikh lab! Awesome collaborative effort with graduate student Oyku! I hope people give it a read!

bioRxivpreprint@biorxivpreprint.bsky.social · 2mo ago

Polynucleotide Phosphorylase Stops Replication Restart by Degrading the RNA within R-loops during Replication-Transcription Conflicts https://www.biorxiv.org/content/10.64898/2026.06.02.729377v1

I am delighted to share our latest preprint: doi.org/10.64898/202.... Fantastic work from my past and present lab members @flofournes.bsky.social, Sandra Martin and Nicolas Pellaton. Also many thanks to our precious collaborators @gruberlab.bsky.social and to @snsf.ch for funding this research.

Join us in Nottingham in September for the UK DNA replication meeting 🧬. Super EarlyBird deadline today and abstract deadline in 1 month. www.eventsforce.net/biochemsoc/f...

Amazing work from the Dewar lab!

James Dewar@jamesmdewar.bsky.social · 2mo ago

🧬 ⛓️‍💥 Our paper is now out in @natsmb.nature.com ! We examined the fate of replication forks that collapse at single-strand DNA breaks (SSBs) in vertebrates 🐸, comparing the consequences of collapse at single vs convergent forks. www.nature.com/articles/s41...

Today we are very pleased to share our latest article available now online @narjournal.bsky.social about the role of Slx4 scaffold and Fun30 (SMARCAD1 homolog) in DNA damage response regulation and replication fork protection against Top1-DNA crosslinks. academic.oup.com/nar/article/...

Our H1 paper is out #ScienceAdvances: www.science.org/doi/10.1126/... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

Check out our latest work in collaboration with @kazu-maeshima.bsky.social and @masaashimazoe.bsky.social We show that H1 is a dynamic, multivalent “liquid-like” glue of chromatin in cells: it bridges multiple nucleosomes & exchanges partners frequently to enable both compaction and flexibility

Kazuhiro Maeshima@kazu-maeshima.bsky.social · 4mo ago

Our H1 paper is out #ScienceAdvances: www.science.org/doi/10.1126/... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

New review in @cp-trendsgenetics.bsky.social on the fascinating world of chromatin organization in Asgard archaea. Very nice joint effort with @jvhooff.bsky.social www.sciencedirect.com/science/arti...

Chromatin organization in Asgard archaea: histones, SMC complexes, and the archaeal roots of eukaryotic chromatin

The genomes of organisms across the tree of life are structurally and functionally organized into chromatin. In eukaryotes, within an organelle called…

sciencedirect.com

“I think it’s a real mistake to forget where the pipeline for all this begins.” John Diffley reflects on the importance of basic research, his mission to decode DNA replication and recreate it in the lab, and the scientific optimism of the 1960s that helped shape it. www.crick.ac.uk/news/2026-03...

We are excited to be recruiting into 3 Associate Professorship's in @oxfordbiochemistry.bsky.social. Come join us as a colleague and benefit from our vibrant and multidisciplinary environment. Reach out to me if you have any questions. Please repost! (tinyurl.com/48deybuu) (tinyurl.com/4pdvjaft).

Do you work on DNA or RNA protein complexes? 🧬 Come to Machines on Genes this June in Crete! ☀️ Super early discount is ending this Sunday so register now! 😃

Alessandro Costa@costalaboratory.bsky.social · 7mo ago

8–11 June 2026 | 📍 Heraklion, Crete 🇬🇷 Get ready for Machines on Genes 2026, the 94th Harden Conference by the @biochemsoc.bsky.social — four days of molecular mechanisms and friendly discussions! 🧬☀️🔬❄️ Organised by @lapassmore.bsky.social, Dana Branzei, me.

Xiaofeng combined 2D live-cell single-particle tracking with 3D simulations to specifically measure the bacterial nucleoid accessibility and viscosity! Our preprint describes how these nucleoid properties respond to cellular processes! Code is available too. www.biorxiv.org/content/10.6...

Papers are like buses... You wait for ages, then two come along at once. Huge congrats to @bornanovak.bsky.social and @jefflotthammer.bsky.social for pushing and driving every aspect of this work, preprinted ~1 year ago to the day (Friday before BPS), now published! www.nature.com/articles/s41...

Accurate predictions of disordered protein ensembles with STARLING - Nature

The deep learning model STARLING can generate accurate ensembles of intrinsically disordered regions of proteins using only protein sequence as input.

nature.com

Borna Novak@bornanovak.bsky.social · last yr.

Excited to announce the newest member of the flock - STARLING (conSTruction of intrinsicAlly disoRdered proteins ensembles efficientLy vIa multi-dimeNsional Generative models). www.biorxiv.org/content/10.1...

To kickstart this new Bluesky account: We're hiring! The Bertolin Lab @dundee.ac.uk is looking for a postdoc to work on DNA-end homeostasis & genome stability, funded by Wellcome Trust Award. Exciting science, fantastic environment, founding role in the lab. Details in the flyer — get in touch! 👇

🧬🔬🎥 @science.org Live-cell single-molecule dynamics of eukaryotic RNA polymerase machineries | Science www.science.org/doi/10.1126/...

Live-cell single-molecule dynamics of eukaryotic RNA polymerase machineries

Eukaryotic gene expression is orchestrated by RNA polymerases (RNAPI, II, and III) and associated factors, yet their real-time dynamics remain obscure. Using single-molecule tracking in living yeast, ...

science.org

We are excited to share that our latest work from the lab aimed at understanding how the tRNA nuclease SLFN11 is activated in response to DNA damage and replication stress has just been published in @natcellbio.nature.com! Open access link: www.nature.com/articles/s41... (1/9)

We’re looking for a postdoc to join the Hendrickson lab! The project will involve dissecting the molecular mechanisms of a fascinating mobile element in our honeybee biocontrol phages. Sound like something you would be interested in? Get in touch! Details: jobs.canterbury.ac.nz/jobdetails/a...

New paper in collaboration with @unterholznerlab.bsky.social @cejkalab.bsky.social IFI16 protects stalled replication forks. www.sciencedirect.com/science/arti...

IFI16 senses and protects stalled replication forks

Replication stress is a key driver of DNA damage and genome instability. Here, we report that replication stress induces an inflammatory response in t…

sciencedirect.com

After 4 years of joint effort of my Lab and Dr. Llorente Team we took for first time SCRaMbLE to a bacterial system! This allowed us to massively reordered the main chromosome of Vibrio natriegens! biorxiv.org/content/10.6... Here's a 🧵

Creating bacterial genomic diversity through large-scale reconfigurations reveals phenotype robustness to organizational genome change

The ability to generate genomic diversity expands opportunities for understanding and engineering biology. Here, we demonstrate on-demand generation of diversity in bacterial genome configurations and...

biorxiv.org

Single-molecule tracking of RNA-DNA hybrid removal enzymes important for lagging-strand replication www.biorxiv.org/content/10.6...

Single-molecule tracking of RNA-DNA hybrid removal enzymes important for lagging-strand replication

The formation of RNA-DNA hybrid (RDH) primers by primase is an essential step in the recruitment of DNA polymerase during replication initiation and for the synthesis of each Okazaki fragment on the l...

biorxiv.org

OPPORTUNITY: McGill Plant Science is looking for an expert in plant pathology, plant breeding, or horticulture in the context of the Canada Impact+ Research Chairs Program (senior established researcher). Contact: www.mcgill.ca/plant/contact Program: lnkd.in/eavfNu7e Please re-post.

Delighted to share a new paper from our lab, a study led by Mathieu Bergé looking at how an endogenous toxin targets replication to induce competence in the pneumococcus. dx.plos.org/10.1371/jour...

A toxin/antitoxin system targeting the replication sliding-clamp induces competence in Streptococcus pneumoniae

Author summary The environment in which bacteria live puts them under a great deal of stress, forcing them to adapt constantly, either temporarily or permanently. Streptococcus pneumoniae, a pathogeni...

dx.plos.org

A cool genetic system to study site-pecific replication fork collapse and repair from the brilliant @winterhalterlab.bsky.social www.nature.com/articles/s41...

Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading - Nature Communications

DNA damage can lead to cell death. Here, the authors show that a simple cut on either strand of DNA can inactivate bacterial chromosome replication. Surprisingly, only a core set of recombination prot...

nature.com

My PhD students Aoi Otsuka @masaashimazoe.bsky.social et al. published @csf-jscb.bsky.social 🎉 Single-nucleosome imaging in an oncogene-inducible human carcinogenesis model shows biphasic chromatin dynamics (1–3 d same; 5–7 d ↑; back by week 4). Congrats! 👉 doi.org/10.1247/csf....

Proud of contributing to this work on archaeal chromosome segregation! Congrats, Rachel and Steve! www.nature.com/articles/s41...

In a new paper, Rashid & Roy characterize a novel RNA-binding helicase, HZL-1. The protein regulates reproductive developmental quiescence in C. elegans through inhibition of key mRNAs, and sits on the axis between conserved AMPK & TOR metabolic signaling pathways. journals.plos.org/plosbiology/...

AMPK modulates a DEAH box RNA-helicase to attenuate TOR signaling and establish developmental quiescence in Caenorhabditis elegans

Caenorhabditis elegans adapts to starvation and other stressors by transiting through the quiescent developmental stage dauer, but the underlying mechanisms remain unclear. This study shows that TOR a...

journals.plos.org