Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by @anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...

How long does it take Caulobacter to adhere? Introducing the Trapezoid 🚨 Guillaume & Sukanya (@brunlabcaulo.bsky.social), et al. capture real-time adhesion with optical tweezers. Now online doi.org/10.1021/acsnano.6c03697 @pubs.acs.org #BacterialAdhesion #Biofouling #Caulobacter #OpticalTweezers

TL;DR: We've identified more than 100 cases of apparent manipulation in Thermo Fisher Scientific's antibody verification data. @sholtodavid.bsky.social @johanduchene.bsky.social reeserichardson.blog/2026/05/28/h...

How much of Thermo Fisher’s antibody data has been manipulated?

We’ve documented more than 100 instances of apparent data manipulation in Thermo’s catalog

reeserichardson.blog

ICYMI: a quick calculation of transmembrane domain-containing proteins in a proteome. If you need to use this info: the code is in the post, or you can cite it since all quantixed posts have their own DOI courtesy of Rogue Scholar. doi.org/10.59350/0ah...

Stephen Royle@steveroyle.bsky.social · 4mo ago

How many proteins in the human proteome have transmembrane domains? And of those, how many examples with n TMDs are there? Couldn't find an answer, so I calculated it from Uniprot data with some #RStats magic (and I did some model organisms while I was at it). 🧪 quantixed.org/2026/04/16/m...

doi.org/10.1038/s415...

🧵 Our new paper is out today in molecularcell.bsky.social! After 7 years of a truly intriguing discovery journey, we can finally share what controls how molecules move inside living E. coli cells. Spoiler: it's not what the field thought. A thread 👇 📄 doi.org/10.1016/j.molcel.2026.03.018

www.pnas.org/doi/10.1073/...

Life-like behavior emerging in active and flexible microstructures | PNAS

Many organisms leverage an interplay between shape and activity to generate motion and adapt to their environment. Embedding such mechanical feedba...

pnas.org

1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵 🔗 www.nature.com/articles/s41... #CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature

Cas9 structures explain topology sensing and off-target activation.

nature.com

Please check out our newest work on fluorogenic speed imager probes, in which we combine the strengths of speed-optimized imagers and fluorogenic imagers. #SuperResolution #DNAPAINT #microscopy www.biorxiv.org/content/10.6...

DNA-PAINT with photocaged DNA labels = PhotoPAINT🔬. Why? No need for washing or mixing 👉🏻 higher accuracy in DNA-PAINT & other super-res methods. Fantastic collab with Alex Heckel & driven by the 'magic team' Nina Kaltenschnee and Marina Dietz 💪 doi.org/10.1002/anie... @goetheuni.bsky.social

Heilemann Lab@heilemannlab.bsky.social · 6mo ago

🔬we have been playing with 'an old tool from (DNA) chemistry' 🧪 and used that for DNA-based confocal/super-res microscopy ... these are the last recordings for the revision and were recorded last night ... 🙏 thanks to R1 for this suggestion:

β-barrel nanopores designed for insertion into thick block copolymer membranes https://www.biorxiv.org/content/10.64898/2026.03.13.711555v1

Neat paper from Schwille lab. They demonstrate constriction of synthetic vesicles using important components of the bacterial cell division machinery, FtsZ and FtsN. They induce strong constriction of Z-rings in vesicles just by adding the cytoplasmic domain of FtsN www.nature.com/articles/s41...

Optimizing spatial organization of FtsZ rings for large-scale constriction in synthetic cells - Nature Communications

Spatially regulated membrane constriction is an important milestone in reconstituting minimal cell division. Here the authors engineer a truncated system where the cytosolic domain of FtsN forms large...

nature.com

New paper alert! Authors introduce 𝗚𝟱𝗠, 𝗮 𝗗𝗡𝗔-𝗣𝗔𝗜𝗡𝗧-𝗼𝗽𝘁𝗶𝗺𝗶𝘇𝗲𝗱 𝗚𝗮𝘂𝘀𝘀𝗶𝗮𝗻 𝗠𝗶𝘅𝘁𝘂𝗿𝗲 𝗠𝗼𝗱𝗲𝗹𝗶𝗻𝗴 𝗮𝗹𝗴𝗼𝗿𝗶𝘁𝗵𝗺 that accurately determines true molecular position, even at the 𝗥𝗮𝘆𝗹𝗲𝗶𝗴𝗵 𝗹𝗶𝗺𝗶𝘁, and 𝗶𝗺𝗽𝗿𝗼𝘃𝗲𝘀 𝗲𝗳𝗳𝗲𝗰𝘁𝗶𝘃𝗲 𝗿𝗲𝘀𝗼𝗹𝘂𝘁𝗶𝗼𝗻 𝗯𝘆 ~𝟳𝟬%. Huge congratulations to all authors! www.nature.com/articles/s41...

Molecular mapping in DNA-PAINT via modified Gaussian Mixture Modeling - Nature Communications

Standard algorithms fail to fully exploit the spatial information in DNA-PAINT. Here, the authors present G5M, an algorithm providing molecular maps by accurately inferring biomolecule positions at di...

nature.com

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells. www.nature.com/articles/s41... rdcu.be/eQVxt

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods

4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

nature.com

I was invited by @focalplane.bsky.social to write a short description about it: focalplane.biologists.com/2025/12/12/a...

A new version of TrackMate with improved integration of key AI algorithms and track edition improvements - FocalPlane

A new version of TrackMate with improved integration of key AI algorithms and track edition improvements -

focalplane.biologists.com

Jean-Yves Tinevez@jytinevez.bsky.social · 8mo ago

The new version of TrackMate, the v8.1 has just been released. It builds upon the recent v8, linked below, and offers two main improvements 👇

A Cinderella story: raising CLIP-tag from the ashes to shine in fluorescence labeling. www.biorxiv.org/content/10.6...

#HappyMicroscopyMonday #microscopycommunity-Join the AIMM user community on Friday, March 6, for a talk by Hannah Somers- “Stop Reinventing the Protocol: MicroHub, a Searchable Hub for Microscopy Knowledge” Learn more: buff.ly/kZSIkSJ

Final version @nature.com of our paper describing unconventional multicellular development in a choanoflagellate inhabiting an extreme environment. A ton of new data since the first @biorxivpreprint.bsky.social preprint (which we've kept updating). A brief 🧵 (carried over from the old place)

Nature Portfolio@natureportfolio.nature.com · 5mo ago

A study in Nature shows that the single-celled form of a tiny, aquatic organism can turn into a multicellular version by three different routes. The discovery adds insight to the possible origins of multicellular life, suggesting a previously unrecognized degree of flexibility. 🧪

🙏 This study was powered by fantastic collaborations — especially with @abelljonny.bsky.social & @joechambers.bsky.social with contribution form @felixmendu.bsky.social @lasergroup.bsky.social @franbottanelli.bsky.social 🔬🧠 www.nature.com/articles/s41...

Avezov lab@avezovlab.bsky.social · 6mo ago

Our new paper's out: FidlTrack—structure-aware single-particle tracking benchmarks/boost SPT fidelity With it we resolve with sub-organelle res. e.g. BACE1 amyloidogenic APP cleavage #Alzheimers, ER exit events, map nanobody binding in realtime in ER/organelles 🔬🧠#SingleMolecule rdcu.be/e3Ris

Continuous mass photometry by single molecule trapping https://www.biorxiv.org/content/10.64898/2026.02.02.703204v1

Ragaller, F., Sjule, E., Urem, Y. B., ..., Blom, H., & Sezgin, E. (2024). Quantifying Fluorescence Lifetime Responsiveness of Environment-Sensitive Probes for Membrane Fluidity Measurements. The journal of physical chemistry. B, 128(9), 2154–2167. #EpithelialMechanics buff.ly/YjCWSfP

A protein called Uninflatable acts as a switch between glial cells growing and glial cells wrapping themselves around axons. 🔗 buff.ly/WRdybO3

Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody. Its binding turns on and off with a magnet. This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos. www.nature.com/articles/s42...

The new version of TrackMate, the v8.1 has just been released. It builds upon the recent v8, linked below, and offers two main improvements 👇

Jean-Yves Tinevez@jytinevez.bsky.social · 9mo ago

We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji. It ships many new features, detailed below, but that are articulated around the following:

📢 Paper alert 📢 Chirality is known to be important for the movement of microorganisms and active matter. In our new paper out today in @natphys.nature.com, we show that chirality is used by malaria parasites to control their motion patterns: doi.org/10.1038/s415... Here comes a 🧵 ... (1/9)

Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡

StayRose: A photostable StayGold derivative redshifted by genetic code expansion

Photobleaching of fluorescent proteins often limits the acquisition of high-quality images in microscopy. StayGold, a novel dimeric GFP recently monomerized through sequence engineering, addresses thi...

jbc.org

Just published in Nat. Neurosci. Led by J.P. Ferrari-Souza, this study used imaging & fluid biomarkers to reveal that microglia modulate Aβ-related astrocyte reactivity, which in turn contributes to AD progression. @erzimmer.bsky.social @natureportfolio.nature.com www.nature.com/articles/s41...

Microglia modulate Aβ-dependent astrocyte reactivity in Alzheimer’s disease - Nature Neuroscience

Microglia influence amyloid-β effects on astrocyte reactivity in the living brain of individuals with Alzheimer’s disease. This phenomenon further contributes to cognitive impairment via tau phosphory...

nature.com

I did not expect that: Large Language Models are invertible: arxiv.org/abs/2510.15511

Language Models are Injective and Hence Invertible

Transformer components such as non-linear activations and normalization are inherently non-injective, suggesting that different inputs could map to the same output and prevent exact recovery of the in...

arxiv.org