jake
@mediocre-jake.bsky.social
Postdoc interested in #ubiquitin #lipid #innateimmunity #E3ligases #endoplasmicreticulum https://findaresearcher.wehi.edu.au/chua.n Postdoc (WEHI) https://www.wehi.edu.au/laboratory/feltham-lab/ PhD (UNSW) https://x.com/brownlabunsw?lang=en&mx=
Interested in non-proteinaceous ubiquitination, induced proximity or glycogen? Then you should have a look at our newest article, “Ubiquitination of glycogen and metabolites in cells and tissues”, just published in Nature: www.nature.com/articles/s41...
Resource @cellcellpress.bsky.social @wehi-research.bsky.social @mediocre-jake.bsky.social The E3-ome gene-centric compendium reveals the human E3 ligase landscape www.cell.com/cell/fulltex...
Now online! The E3-ome gene-centric compendium reveals the human E3 ligase landscape
The E3-ome gene-centric compendium reveals the human E3 ligase landscape
The E3-ome defines the human repertoire of ubiquitin E3 ligases, creating a unified resource that maps their diversity across the ubiquitin and ubiquitin-like systems. By consolidating fragmented knowledge, this framework provides a foundation for studying ubiquitin signaling and accelerating discovery.
dlvr.it
New Online! STING signalling as a mediator between lipid metabolism and innate immunity
STING signalling as a mediator between lipid metabolism and innate immunity
Nature Reviews Molecular Cell Biology, Published online: 30 March 2026; doi:10.1038/s41580-026-00967-zThis Comment discusses the bidirectional interplay between STING signalling and lipid homeostasis, highlighting the emerging therapeutic relevance of this regulatory network.
dlvr.it
Vila, Messaoud-Nacer, Taffoni, @nadinelaguette.bsky.social et al show that DNA-PKcs, a major DNA damage response actor, interacts w/ cyclic dinucleotides through its kinase domain, ensuring CDN-associated signal termination, while CDNs inhibit DNA-PKcs catalytic activity rupress.org/jem/article/...
Ubiquitin ligase CHFR impairs Tie2 signaling via K48-linked ubiquitylation and degradation of Akt1 in endothelial cells https://www.biorxiv.org/content/10.64898/2026.03.31.715582v1
So delighted to have been part of this fantastic collaboration spearheaded by Ngee Kiat 'Jake' Chua (蔡毅杰) and Rebecca Feltham! Congratulations on bringing together so many experts in the field to make such an important resource 🎉 it has been a pleasure! www.sciencedirect.com/science/arti...
The E3-ome gene-centric compendium reveals the human E3 ligase landscape
To define and systematically characterize the human E3 ubiquitin ligase (E3) landscape, we generated the E3-ome, a compendium of E3s encoded by the hu…
sciencedirect.com
Amazing to be able to work with the team in Otago Many fundamental discoveries on what we know about RING E3s were made by Peter Mace at Catherine Day in New Zealand Always a pleasure to go back to basics and apply it with a modern lens @otagobiochemist.bsky.social
Otago Biochemistry@otagobiochemist.bsky.social · 4mo agoLatest publication with a contribution from Otago Biochemistry. The E3-ome gene-centric compendium reveals the human E3 ligase landscape www.sciencedirect.com/science/arti...
Out on the 2nd April 2026 issue of Cell Should really build a Bluesky thread around the E3-ome development Thanks for sharing! It wasn't a straightforward path so many more cool E3s to be uncovered!
Waggoner Lab@labwaggoner.bsky.social · 4mo agoResource @cellcellpress.bsky.social @wehi-research.bsky.social @mediocre-jake.bsky.social The E3-ome gene-centric compendium reveals the human E3 ligase landscape www.cell.com/cell/fulltex...
Congratulations Jake @mediocre-jake.bsky.social, Bekky Feltham and team on their phenomenal effort in putting together the E3-ome, published in Cell www.cell.com/cell/fulltex.... An extremely valuable resource for ubiquitin researchers.
The E3-ome gene-centric compendium reveals the human E3 ligase landscape
The E3-ome defines the human repertoire of ubiquitin E3 ligases, creating a unified resource that maps their diversity across the ubiquitin and ubiquitin-like systems. By consolidating fragmented know...
cell.com
WEHI (The Walter and Eliza Hall Institute of Medical Research)@wehi-research.bsky.social · 5mo agoWEHI researchers have led a major global effort to create the first authoritative atlas for E3 ligases, resolving more than 18 years of inconsistencies in the ubiquitin field. Study led by Dr Ngee Kiat ‘Jake’ Chua & Dr Rebecca Feltham in @cellpress.bsky.social www.wehi.edu.au/news/new-enz...
A breakthrough in lipid imaging: spatial lipid distributions in organelles! Fantastic work from @nadlerlab.bsky.social 🤯
André Nadler@nadlerlab.bsky.social · 5mo agoIt’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density. Lipid-CLEM, now out in @natcellbio.nature.com brought to you by @mathilda95.bsky.social changes that: www.nature.com/articles/s41...
Glückwunsch, Bruderherz! Really outstanding work by @bartellab.bsky.social and Schulman Lab. This is how transatlantic research is done 🇺🇸🇩🇪.
Jakob Farnung@jakobfarnung.bsky.social · 5mo agoThe E3 ubiquitin ligase mechanism specifying target-directed microRNA degradation (TDMD) is now published! 🎉🍾 We, @bartellab.bsky.social and Schulman lab, describe how 2-RNA factors control protein degradation by recruiting an E3 ligase. @mpibiochem.bsky.social www.nature.com/articles/s41...
Cofilin-1 is a redox-sensitive guard of the NLRP3 inflammasome @natimmunol.nature.com www.nature.com/articles/s41...
Ever wonder how IDRs and folded domains work together to control function? Using ubiquilins, multidomain proteins involved in protein degradation, we show in our new preprint that IDRs aren’t just linkers, but regulate ensemble and function. Read it here: doi.org/10.64898/202...
Intramolecular interactions between folded and disordered regions shape ubiquilin structure and function
Multidomain proteins consist of folded domains connected by intrinsically disordered regions. The flexibility afforded by the disordered regions coupled to the structure and surface chemistry of folded regions allows for unique structural and functional features in these proteins. Yet how intramolecular interactions between disordered regions and folded domains affect multidomain protein structure and function remain poorly understood. Here we use a range of biophysical and computational approaches to measure the intramolecular interactions between the folded domains and disordered regions of ubiquilins (UBQLNs) - essential components of protein quality control that shuttle poly-ubiquitinated client proteins to proteasomal degradation or autophagy. Starting with the yeast UBQLN homolog Dsk2, we find that interactions between two folded domains located at the opposite ends of UBQLN bring about a closed conformation. The prevalence of this closed conformation, however, is modulated by intramolecular interactions involving the disordered regions and folded STI1 domain at the center of the protein. Simulations and analysis of UBQLN homologs across multiple eukaryotic lineages reveals that these disordered:folded domain interactions exist in some UBQLN homologs but are absent in others, indicating possible fundamental differences in function among proteins with the same multidomain architecture. ### Competing Interest Statement The authors have declared no competing interest.
biorxiv.org
UK Proteostasis Meeting 2026 – Reminder: Register Today 📅 20–21 July 2026 at The Francis Crick Institute, London 🗓 Abstract deadline: 1 May 2026 💷 Fees: £45 (Student/Postdoc) | £75 (Group Leader) 🔗 Register: forms.gle/dWz2qztKgftB... 🔗 Payment: www.eventbrite.co.uk/e/uk-proteos...
In this Perspective, North, @doroteaartscience.bsky.social, Shoemaker et al. highlight the rapidly expanding role of LC3-interacting regions in autophagy. rupress.org/jcb/article/... 📕 Part of #Autophagy 2026 👉 rupress.org/jcb/collecti... #GRC
Scientists: How long have you waited for journal editors to decide whether the journal sends your manuscript out for review? I’m at 2+ months with a “prestigious” journal — not easy when working in a competitive field. What’s the longest you’ve experienced? #AcademicPublishing #PeerReview
1/3 Thrilled to see our paper led by Kirandeep Deol is out in Nature Structural & Molecular Biology! CRISPR screens to uncover a role for vitamin B2 metabolism in stabilizing FSP1 via FAD, revealing a new way vitamins regulate ferroptosis. www.nature.com/articles/s41...
Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis - Nature Structural & Molecular Biology
Here, Deol et al. use genetic screens in gene-edited reporter cell lines to identify regulators of ferroptosis suppressor protein 1 (FSP1) expression and stability. They show that vitamin B2 metabolis...
nature.com
Lipids Meet Immunity: Metabolic Control of cGAS-STING Having worked in lipid metabolism and cGAS-STING, I have an appreciation for the regulation of cGAS-STING by lipids, which I co-wrote. www.mdpi.com/2813-7086/3/... @wehi-research.bsky.social @sahmri.bsky.social
Lipids Meet Immunity: Metabolic Control of cGAS-STING Having worked in lipid metabolism and cGAS-STING, I have an appreciation for the regulation of cGAS-STING by lipids, which I co-wrote. www.mdpi.com/2813-7086/3/... @wehi-research.bsky.social @sahmri.bsky.social
This is a video summarizing our recent paper (www.nature.com/articles/s41...) youtu.be/eNY2CRlYTRo
A multivalent adaptor mechanism drives the nuclear import of proteasomes - Nature Communications
Nuclear protein homeostasis relies on proteasome import into the nucleus. Here the authors identify how assembled human proteasomes are transported across the nuclear pore complex and reveal a mechani...
nature.com
New VCP/p97 review article. Report on the excellent VCP meeting at CalTech last year and great overview on VCP and VCP disease. doi.org/10.1016/j.nb...
Thrilled to be hosting Brenda Schulman, @aakritijain.bsky.social, @crismayorruiz.bsky.social @davidbalchin.bsky.social, @hemmo-lab.bsky.social, Ian Ganley, @kirsteinlab.bsky.social, Juliette Fedry, @elliottlab.bsky.social & @plantophagy.bsky.social - BIG THANKS for accepting the invitation 🙏
VCP/p97’s accessory adapters (FAF1, FAF2, UBXN7) boost unfolding and subsequent proteasomal degradation. #ubiquitin. Our paper is out now: Science Advances www.science.org/doi/10.1126/...
The accessory adapters FAF1, FAF2, and UBXN7 accelerate proteasomal degradation by increasing prior p97-mediated substrate unfolding
A group of accessory adapters positions the p97 adapter Ufd1 to boost substrate unfolding and subsequent proteasomal degradation.
science.org
I am incredibly excited to share that I will start my independent lab at the @unidue-zmb.bsky.social at the @unidue.bsky.social as Junior Professor of Cellular Biochemistry. Research in my lab has the goal to decipher the ubiquitin code! There are multiple open positions! (1/3)
Allosteric PROTACs: Expanding the Horizon of Targeted Protein Degradation | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...
Allosteric PROTACs: Expanding the Horizon of Targeted Protein Degradation
Proteolysis-targeting chimeras (PROTACs) have transformed the concept of chemical intervention in biological systems by co-opting the ubiquitin–proteasome system to selectively degrade proteins. A key...
pubs.acs.org
The date on the poster says February 5…should be March 5, right?
Super excited to present out work at @mitotalks.bsky.social this Thursday!! Hope to see you there!
@mitotalks.bsky.social · 5mo agoWe talk a lot about mitochondrial stress, but how about how do they keep themselves balanced? Good thing our speaker @nieminm.bsky.social knows everything about that, and will tell all on the next MITOtalk. Usual time and place. See you there?
Congratulations to the winners of this year's Art of Science Image contest at #bps2026 🥇 1st Place: Yupeng Li, University of Illinois Urbana-Champaign 🥈 2nd Place: Jessica Heebner, Thermo Fisher Scientific 🥉 3rd Place: Sohaib Abdul Rehman, Harvard University
Temporal proteomic and phosphoproteomic dynamics during neuronal differentiation of iPSC www.science.org/doi/10.1126/...
