Very happy to share my postdoc paper is out now in @natcellbio.nature.com titled "Tissue flow acts as a guidance cue for immune cell polarization and directional migration". If you love imaging and immune cells, this is for you! #microscopymonday www.nature.com/articles/s41... A 🧵: 1/n

This is why we love #CellMigratuon ! This beautiful story is now out, very interesting role for RhoA and also the cover, Neutrophils on the 'Rho'ad Congrats to all authors, especially to former colleagues and friends Full @science.org www.science.org/doi/10.1126/...

Adaptive motility enables neutrophils to rapidly navigate confined capillaries

Neutrophils maintain migration speed in confined capillaries through adaptive actomyosin remodeling.

science.org

Pablo J. Sáez@pjsaez.bsky.social · 2y ago

How from the #preprint, super cool story on the role of #cytoskeleton by my former lab Spearheaded by dear friend and cell biologist extraordinaire Mathieu Deygas (Piel, Vargas lab @institutcurie.bsky.social) Data from in vitro to in vivo. Full at www.biorxiv.org/content/10.1... #scicomm #SciSky

Check out our preprint on the structural mechanisms of how LEM2 and ESCRTs seal the nuclear envelope. Awesome collaboration with @karenpalacior.bsky.social who brought my structures to life 💃🔬❄️🌀 @hummerlab.bsky.social www.biorxiv.org/content/10.6...

Any1 is a #phospholipid scramblase involved in #endosome biogenesis. From Jieqiong Gao, @labvanni.bsky.social, Christian Ungermann and colleagues: rupress.org/jcb/article/... 📕 From our #Lipid and #Membrane collection: rupress.org/jcb/collecti... #EMBOLipidCode

🚨New preprint alert 🚨 "Pancreatic cancer cells breach endothelial barriers through protrusion-driven invasion or endothelial retraction" www.biorxiv.org/content/10.6...

Immune cells are fascinating 🤩 . Here, an embryonic myeloid cell (pre tissue-resident mac) ( 🔴 ) is seen in its (almost) natural tissue environment ( 🔵 ) of ectodermal cells. The cell is in a 2.5D-3D environment and looks it is being "swallowed up" by the surrounding cells 😁. #FluorescenceFriday.

I never get bored of watching my favorite lipid, PI(4,5)P2 - especially in real time! In this case imaged with a HaloTag-biosensor and 50 pM JF646.

PRISM: A Plasmid-based Reporter for Intracellular Spectral Microscopy by Jonathon Nixon-Abell and team: www.biorxiv.org/content/10.6...

loved this read, so touching!! 🥹🥹

MRC Laboratory of Molecular Biology@mrclmb.ac.uk · 5d ago

50 years after Barbara Pearse’s seminal discovery of clathrin, Frances Brodskey and Scottie Robinson met Barbara to reflect on the lasting impact her work has made to the field of cell biology. Read the interview here: onlinelibrary.wiley.com/doi/10.1111/... #LMBAlumni @cellbiol-mrclmb.bsky.social

Really cool! Smaller than GFP and reversibly binding last-generation rhodamine dyes. De novo pan-rhodamine binders for fluorescence microscopy from mammalian cells to extremophiles www.cell.com/cell/fulltex... Congrats @bohuang.bsky.social @rhodamine110.bsky.social @mullinslab.bsky.social et al

☕This Review highlights advanced imaging methods and available biosensors that can be used to elucidate the composition and dynamics of membrane contact sites. 👉https://rdcu.be/4uDgJ3upkD1a www.nature.com/articles/s41...

Advanced imaging of membrane contact sites - Nature Cell Biology

The detailed study of membrane contact sites is challenging owing to their small size and highly dynamic nature. This Review highlights advanced imaging methods and available biosensors that can be us...

nature.com

ClC-7 links PIKfyve inhibition to Rab-dependent LRRK2 activity at lysosomes www.biorxiv.org/content/10.1...

ClC-7 links PIKfyve inhibition to Rab-dependent LRRK2 activity at lysosomes

Increased activity of leucine-rich repeat kinase 2 (LRRK2) confers Parkinson's disease risk. LRRK2 dynamically localizes to lysosomal membranes in response to various stresses, yet the mechanisms by w...

biorxiv.org

#Integrins and #tetraspanins mediate CD36-#actin coupling and regulate CD36 organization and signaling. New study from Soma Jana, Huong-Tra Ngo, Aparajita Dasgupta, Khuloud Jaqaman and colleagues (UT Southwestern Medical Center): rupress.org/jcb/article/... #Biophysics #CellSignaling

Happy #FluorescenceFriday! This is the cortical actin cytoskeleton of a macrophage imaged using eSRRF and @spirochrome.com's SPY555-FastAct_X.

My blog post explaining the situation in greater detail: reeserichardson.blog/2026/08/25/a... 2/🧵

At least 15 companies are selling antibodies using faked validation data

Popular vendors’ catalogs feature more than 18,000 manipulated images

reeserichardson.blog

www.nature.com/articles/s41... Substrate curvature and stiffness equivalence in governing cell mechanosensing

Membrane PI(4,5)P2 and ErbB2 abundance regulate ErbB receptor signaling through receptor oligomerization and activation https://www.biorxiv.org/content/10.64898/2026.08.24.746611v1

journals.plos.org/plosbiology/... Wow, this is kind of significant. GFP or FLAG tag Coronin makes the protein relocalise more to F-actin, while endogenous proteins don't. The paper also says that Coronins dont do actin-related functions. Wonder what'd happen if the linkers for the tags are longer?

Reassessment of the roles of coronin proteins as actin effectors and in signaling

Coronins are evolutionarily conserved proteins involved in many biological processes, but exactly how coronin proteins exert their function is unclear. This study shows that peptide or fluorescent pro...

journals.plos.org

I think I could spend the next lifetime at the microscope and never get sick of it ....

Check out this new paper from Stefan Lechner's lab showing with minflux nanoscopy how PIEZO1 channels cluster and invagininate the membrane. Glad we could make a small contribution to this nice story! www.science.org/doi/full/10....

Cluster nanoarchitecture and structural diversity of PIEZO1 at rest and during activation in intact cells

MINFLUX nanoscopy reveals subcellular distribution and conformational diversity of PIEZO1 channels in intact cells.

science.org

a good review and some tea makes a geat evening!! so lucky

I missed this beautiful work showing that myelin compaction depends on actin disassembly. It has SMLM, correlative AFM with a clever fluorescent nanoscale reporter of compaction, in vivo cell-specific actin disassembly using deActs… check it out! www.biorxiv.org/content/10.6...

Take a look at our new paper on the structure of the EHD2 that, upon oligomerization, forms rings that remodel the plasma membrane and form caveolae. Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...

Lipids build the membranes of all cells, but identifying their precise functions is extremely challenging. Here’s our attempt to change that: How to find and understand lipid-protein interactions, by Katelyn Cook & company. www.biorxiv.org/content/10.6...

www.nature.com/articles/s41...

Resolving endogenous protein organization in cells with nanometer resolution - Nature Communications

Eilts and colleagues combine a double-homogenisation expansion microscopy approach with dSTORM, revealing molecular details of endogenous protein complexes, including microtubules, clathrin lattices a...

nature.com

New preprint! How does a cell "know" how big to make its nucleus? This has puzzled biologists since the 1800’s. We show the answer is physics: colloid osmotic pressure. 🧵1/5

bioRxiv Biophysics@biorxiv-biophys.bsky.social · 2mo ago

Nuclear size and physical properties of the nucleoplasm are determined by colloid osmotic pressure at the nuclear envelope https://www.biorxiv.org/content/10.64898/2026.07.21.739918v1

Controlled blinking of upconverting nanoparticles - no bleaching means attractive potential as an SMLM probe! www.nature.com/articles/s41...

💡New Preprint! How does a cell control which way a membrane bends? ESCRT-III has the remarkable ability to reshape membranes and drive reverse-topology fission, yet a fundamental question has remained: what determines the direction of membrane remodeling? www.biorxiv.org/content/10.6...

Direction of ESCRT-III-dependent membrane bending emerges from bilayer asymmetry

In cells, ESCRT-III is unique in mediating fission of membrane necks from inside, a process called reverse-topology fission. Yet, in vitro, the complex primarily assembles outside membrane necks and m...

biorxiv.org

A new, rather unexpected glycolipid class. Very well done study. Given how they look structurally, I think it makes a lot of sense to test these lipids against a panel of GPCRs. Lipid biology keeps surprising me. I like that. www.biorxiv.org/content/10.6...

New Toettchlab paper alert! Harrison Oatman just published his beautiful work combining software design for "smart" microscopy+optogenetics and some really beautiful work understanding EGFR-driven collective cell migration. www.cell.com/cell-systems...