Really happy to see this work published in NAR, from Hideki Taguchi's lab at Institute of Science Tokyo. First authors Soyoka Sakamoto and Hayato Ito did an amazing job bridging in vitro translation studies with live-cell single-mRNA imaging. academic.oup.com/nar/article/...

Upstream non-AUG initiation and ribosomal −1 frameshifting in ATXN8OS CTA/CTG repeat-associated translation

Abstract. Microsatellite repeat expansions contribute to the pathogenesis of many neurodegenerative disorders. In spinocerebellar ataxia type 8 (SCA8), abn

academic.oup.com

New Toettchlab paper alert! Harrison Oatman just published his beautiful work combining software design for "smart" microscopy+optogenetics and some really beautiful work understanding EGFR-driven collective cell migration. www.cell.com/cell-systems...

New paper! How do RNAs "know" where to go inside a cell? We dug into the sequence elements that route RNAs to the right place. It turns out that, in mammals, they're surprisingly massive (>200 nt), multipartite, and wonderfully complicated. 🧵

Excellent, concise summary of the threats of recent OMB proposal and suggested action items. DEADLINE TO SUBMIT PUBLIC COMMENTS IS JULY 13, 2026 AT 11:59 PM EASTERN TIME elizabethginexi.substack.com/p/what-we-ne...

What We Need to do NEXT: OMB’s Proposed Federal Financial Assistance Rule (OMB-2026-0034)

What OMB’s Proposed Federal Financial Assistance Rule Means in Practice

elizabethginexi.substack.com

TL;DR: We've identified more than 100 cases of apparent manipulation in Thermo Fisher Scientific's antibody verification data. @sholtodavid.bsky.social @johanduchene.bsky.social reeserichardson.blog/2026/05/28/h...

How much of Thermo Fisher’s antibody data has been manipulated?

We’ve documented more than 100 instances of apparent data manipulation in Thermo’s catalog

reeserichardson.blog

The paper is now published in Current Protocols!! 🎉🥳 currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1... Some personal story below 👇 , and technical details in the linked post that featured the preprint 👉

Automated Optimization of Bacterial Tracking Pipelines With TrackMate 8

Quantitative analysis of bacterial dynamics in time-lapse microscopy requires robust tracking pipelines, yet selecting and optimizing algorithms for specific experiments remains challenging. Indeed, ...

currentprotocols.onlinelibrary.wiley.com

Jean-Yves Tinevez@jytinevez.bsky.social · 6mo ago

New preprint alert! We developed an automated framework to optimize tracking pipelines for bacteria, along with a nice integration of tools for bacteria imaging, in the recently released TrackMate 8 (Fiji plugin). www.biorxiv.org/content/10.6... 👇🧵

i visited DSHB @dshb-antibodies.bsky.social 2 years ago, incredible how such a critical community scientific resource is run so efficiently, economically and humbly. they maintain 1000s of mAbs at cost, and are fully self-supported from just a couple lab rooms!

Mark Peifer (He, him)@peiferlabunc.bsky.social · 2mo ago

By the way, @dshb-antibodies.bsky.social is a non-profit that sells quality antibodies to many proteins at exceptionally reasonable prices, without AI generated faux-validation images. Check them out. dshb.biology.uiowa.edu

I'll be writing a few posts about new features in Micro-Manager. If you are interested in those, please follow @micro-manager.bsky.social.

@micro-manager.bsky.social · 3mo ago

Development of Micro-Manager code has accelerated quite a bit with availability of the new generation of AI coding tools (and the need to provide functionality for microscopes in our core at UCSF). Follow these developments here: nicost.github.io/microscope-t...

For 40 years the 8-nt ‘Kozak Sequence’ was thought to mark sites of translation initiation. In a new study, we revise this model by identifying an ~80-nt sequence—the extended Translation Initiation Sequence (eTIS)— that guides ribosomes to correct start sites. 🧵 www.biorxiv.org/content/10.6...

Wonderful opportunity to hear about the latest progress in fluorescent probe technology ⬇️

Janelia Conferences@janeliaconf.bsky.social · 6mo ago

📢 Applications are open! Join us in October to explore next-generation fluorescent proteins, biosensors & imaging advances + machine learning and computational approaches to accelerate design. 🛏️ Meals & lodging covered; no registration fee Apply by May 21 ➡️ janelia.news/FPS26 @agtebo.bsky.social

Single-mRNA imaging and modeling reveal coupled translation initiation and elongation rates

Single-mRNA imaging and modeling reveal coupled translation initiation and elongation rates

Mammalian cells dynamically scale translation initiation to match distinct elongation rates, preventing ribosome crowding and preserving protein synthesis homeostasis across diverse transcripts.

buff.ly

Hey y’all 👋 repost magic appreciated. The Solecki lab is recruiting multiple postdocs at St. Jude for a chromatin imaging project at the edge of live-cell imaging, neuronal cell biology, chromatin regulation, and quantitative image analysis. Ever dreamed of touring chromatin like this? We CAN 🔥

The hidden rules behind mRNA transport. A collaborative study between @simonbullock11.bsky.social & @carter-lab.bsky.social’s groups has found a structural code that explains how cells selectively transport mRNAs. Read more: mrclmb.ac.uk/news-events/... #LMBNews @cellbiol-mrclmb.bsky.social 🧪

A self-localized ultrafast pencil beam can be applied for near-diffraction-limited, high-throughput volumetric imaging in intact tissues and in vitro models. www.nature.com/articles/s41...

Self-localized ultrafast pencil beam for volumetric multiphoton imaging - Nature Methods

A self-localized, ultrafast pencil beam can be readily introduced into multiphoton microscopes and applied for near-diffraction-limited, high-throughput volumetric imaging in intact tissues and in vit...

nature.com

New preprint alert! Together with Tetsuya Yamamoto at Hokkaido University, we propose a “polymer micelle” model of transcriptional bursting. www.biorxiv.org/content/10.6...

Meet MagLuc: a magnetically-controlled luminescent protein. This is a foundational tool for remote control of biological processes, also known as "magnetogenetics". Can you tell when the magnetic field turns on and off? From @mariaingaramo.bsky.social and the team at @nonfictionlabs.bsky.social

Two weeks left for abstract submission - please do register; it's a great UK meeting, and lots of opportunities for ECRs to share their work 👇

Chris H. Hill@chillzaa.bsky.social · 4mo ago

The @biochemsoc.bsky.social Translation UK 2026 meeting will be at @universityofleeds.bsky.social from 30th June - 2nd July. Fantastic keynote speakers including @jbquerido.bsky.social, @nickywhiffin.bsky.social, Nicolas Locker Register and submit your abstract here: tinyurl.com/muy5kvax

💥 Paper published 💥 Curious how #SARS2 has changed since jumping into humans? We were too! journals.plos.org/plosbiology/... 🧵 🧵 🧵

Evolution of a truncated nucleocapsid protein enhances SARS-CoV-2 fitness by suppressing antiviral responses

SARS-CoV-2 has evolved to optimize its replication fitness in the human host. This study shows that a nucleocapsid gene mutation enhances production of a truncated protein that boosts viral fitness in...

journals.plos.org

Please RT! The Dept of Chemistry at UC Irvine invites applications for a tenured faculty position in Synthetic Organic Chemistry. Deadline: July 1, 2026. More information about the position can be found here: recruit.ap.uci.edu/JPF10109 @uciphyssci.bsky.social

Professor in Synthetic Organic Chemistry

University of California, Irvine is hiring. Apply now!

recruit.ap.uci.edu

there goes the central dogma, i guess. is nothing sacred anymore?

Maxim Greenberg@maxvcg.bsky.social · 4mo ago

There are Science papers and then there are *I just dropped and shattered my mug of coffee Usual Suspects style* Science papers www.science.org/doi/10.1126/...

Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

nature.com

My first, first author paper is now on #ScienceAdvances ! Linker histone H1 is a liquid-like "glue" condensing chromatin, which revises textbooks! 📖✨ science.org/doi/10.1126/sc… Huge thanks to @kazu-maeshima.bsky.social , for supervision. Amazing collab with @rcollepardo.bsky.social ’s group! 1/

My first PhD student‘s, Kavan Gor, paper on 5-color single-molecule imaging of co-transcriptional RNA folding is out in @science.org Advances! He finds how RNA modification enzymes, antisense oligonucleotides and ribosomal proteins re-route nascent RNA folding. See more details in EMBL post below!

EMBL@embl.org · 4mo ago

(1/2) EMBL researchers led by @olivierduss.bsky.social have developed a microscopy technique that enables them to observe individual RNA molecules being produced and folding into different shapes. Learn more: www.science.org/doi/10.1126/...

No Kings Fort Collins

A high-affinity split-HaloTag for live-cell protein labeling. And much more. www.nature.com/articles/s41...

A high-affinity split-HaloTag for live-cell protein labeling

Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...

nature.com

And in yesterday's journal club, we discussed this nice paper on transforming antibodies into intrabodies from @tim-stasevich.bsky.social et al. The authors put together a pipeline comprising ProteinMPNN to design a stabler protein sequence for the scaffold while keeping the antigen-binding loops.

AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications

AI-powered pipeline converts antibodies into functional intrabodies, enabling live-cell imaging of peptides and histone marks.

science.org

1/ 🚀 Excited to release: napari-mcp - agentic control of napari from any MCP-capable AI assistant! Use it for interactive image processing, analysis, and visualisation! Really cool project from @ilan-theodoro.bsky.social from my team! #napari #bioimageanalysis #MCP @biohub

🚨📰Now online in its full form www.nature.com/articles/s41...

FidlTrack: high-fidelity structure-aware single particle tracking resolves intracellular molecular motion in organelles sensing APP processing - Nature Communications

Tracking fast molecules in crowded organelles is error-prone, obscuring dynamic processes like Alzheimer’s secretase activity or secretory sorting. Here, authors present FidlTrack, a structure-aware m...

nature.com

Avezov lab@avezovlab.bsky.social · 6mo ago

Our new paper's out: FidlTrack—structure-aware single-particle tracking benchmarks/boost SPT fidelity With it we resolve with sub-organelle res. e.g. BACE1 amyloidogenic APP cleavage #Alzheimers, ER exit events, map nanobody binding in realtime in ER/organelles 🔬🧠#SingleMolecule rdcu.be/e3Ris

Probing cancer-specific vulnerabilities? The Yeo Lab's RBP CRISPR KO In Vivo Library targets 206 RNA-binding proteins prioritized from screens in KRAS-, BRCA1-, and MYC-driven cancers. Compact size + dox-inducible Cas9 make it perfect for in vivo screens in tumor xenografts.

Kinetic proofreading as a mechanism for transcriptional specificity: www.biorxiv.org/content/10.6... Very nice follow up to their review from last year genesdev.cshlp.org/content/39/1... Non-equilibrium models may be key to understanding specificity in transcriptional regulation!