The final version of @florentwaltz.bsky.social's Chlamy chlororibosome "hat" is now online at @natplants.nature.com 🌾 📜 nature.com/articles/s41477-026-02361-1 🧪🔬 #TeamTomo The charge complementarity in the arm domain is striking. Blue & red proteins wrapped around each other, like a barber pole💈

Florent Waltz@florentwaltz.bsky.social · 6mo ago

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

OK— so @phaips.vd.st & @florentwaltz.bsky.social have been testing extensively, and it's pretty clear that MissAlignment may be the best thing to happen to #TeamTomo in the last couple years. One issue: we have to re-process all our data again, hopefully for the last time 😅. Congrats and THANK YOU!!

Cryo-ET is often framed as a tool for in situ protein structure. But what if the real revolution is contextualization? I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures. tinyurl.com/localmembrane

Think globally, act locally: Redefining organellar membrane environments through cryo-electron tomography

Early enthusiasm for the “cellular revolution” in cryo-electron tomography (cryo-ET) was largely driven by the promise of resolving protein structures…

sciencedirect.com

It's out! 🥳 In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET. 📝 Paper: www.nature.com/articles/s41... 🧵 Thread: bsky.app/profile/madi... #teamtomo #proteomics #AlphaFold

In situ architecture of plasmodesmata in Physcomitrium patens resolved by cryo-electron tomography - Nature Plants

Using cryo-electron tomography, the authors reveal how plasmodesmata (plant cytoplasmic bridges) are gated by cell wall remodelling and how helical protein assemblies scaffold internal membranes, with...

nature.com

Marcel Dickmanns | 🌿❄️🔬@madic.bsky.social · last yr.

New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Thrilled to share the updated video of my paper in Nature with Maciej (under supervision of @simonemattei.bsky.social & @jomaalab.bsky.social) Proud to use Cryo-ET to uncover how hibernation factors are key to translation restart! To model PTMs in situ and render animations fulfilled my PhD dream!🥹🎉

EMBL@embl.org · 3mo ago

Despite its name, SNOR, a newly discovered protein, is hardly asleep on the job. EMBL researchers’ newest technologies allowed their scientific collaboration to discover a tiny protein with a big role in awakening cells from dormancy to restart protein synthesis. www.embl.org/news/science...

Oxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteria www.biorxiv.org/content/10.6... Use of isotope labels and cryo-CLEM-FIB-SIMS to study microbial communities by Hannah Ochner. Collaboration with @kiranrpatil.bsky.social @jmghigolab.bsky.social

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

Big news for correlative cryogenic SMLM/ET! With my colleague Soheil, we (Mahamid and @jonasries.bsky.social labs) indicate lamella-specific illumination can allow 500-1500x higher laser intensities, opening massive new possibilities for correlative imaging #teamtomo www.biorxiv.org/content/10.6...

See also our preprint about the microscope itself, which went up earlier this afternoon! www.biorxiv.org/content/10.6...

Amazing paper. A megadalton enzyme complex that: - combines both electron bifurcation AND confurcation steps - has a new type of iron-sulfur cluster - a new kind of weird electron transfer path - super charges electrons to reduce aromatic rings - also powers respiration 😳

Ben Engel@cellarchlab.com · 4mo ago

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬 Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

Mother Nature is still the undisputed Queen of organic chemistry!

Tomas Pascoa@tomaspascoa.bsky.social · 4mo ago

1/ Excited to share our preprint! 🥳 Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine www.biorxiv.org/content/10.6...

We @embl.org wrote a short preprint about optimizing for maximum completeness in #cryoet analysis. We looked at template matching, VPP imaging, and tilt-series refinement parameters, and why averaging ribosomes helps even if you aren’t interested in their biology 🏭 www.biorxiv.org/content/10.6...

How do “LEGO-like” electron-bifurcating modules combine to drive degradation in BTEX-contaminated ecosystems? Check out our latest preprint, where we use cryo-EM and cryo-ET to reveal how the 1 MDa BCRII complex powers extremely endergonic aromatic ring reduction. www.biorxiv.org/content/10.6...

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬 Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

Tomas Pascoa@tomaspascoa.bsky.social · 4mo ago

1/ Excited to share our preprint! 🥳 Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine www.biorxiv.org/content/10.6...

1/ Excited to share our preprint! 🥳 Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine www.biorxiv.org/content/10.6...

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

We identified the maltoside detergent DM as an effective and easy solution to address preferred orientation in cryo-EM. Helped me switch from graphene grids. Special congrats to Maria Rafiq! @landerlab.bsky.social @nanigrotjahn.bsky.social @wisemanlab.bsky.social #cryoEM doi.org/10.64898/202...

Our latest @biorxivpreprint.bsky.social: "Prion-like transmission of human tau strains in the mouse brain" 🥳 With Michel Goedert and Masato Hasegawa. www.biorxiv.org/content/10.6...

ROCKET 🚀 inference-time optimization of AlphaFold to fit structural data is published! rdcu.be/fa9YH Since our preprint, we’ve pushed it to regimes where other methods break: low resolution, weak signal, real experimental edge cases. Here’s what we learned: 1/15

AlphaFold as a prior: experimental structure determination conditioned on a pretrained neural network

Nature Methods - ROCKET improves experimental structure elucidation by integrating implicit structural knowledge from OpenFold, a trainable reimplementation of AlphaFold2, with X-ray...

rdcu.be

#TeamTomo if you want to use CTF-corrected 3D subtomos from AreTomo3 in RELION v.5.1 you can use this updated script: github.com/Phaips/aretomo3torelion5

Meet Stoic from @daniil-litvinov.bsky.social and @ninjani.bsky.social: embeddings to predict stoichiometry of protein complexes from sequence fast and accurately 🧬🧩💻🤩 www.biorxiv.org/content/10.6...

Excited to announce I’m starting a new lab at @gmivienna.bsky.social (Vienna BioCentre) in Sept 2026 We study how carbon fixation is inherited and maintained in algae using cryo-ET, imaging, environmental sampling&more Looking for people who want to build the lab together, get in touch! #teamtomo

I've written a review on what I think is an extremely exciting direction in cryo-EM: Cryo-EM of endogenous membrane proteins in their native lipid bilayer Open access in Quarterly Reviews of Biophysics: doi.org/10.1017/S003...

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

Check out this cool thread on the latest preprint by Gaurav Kumar, @james-r-barrett.bsky.social, @phaips.vd.st from our lab and colleagues, dissecting the physics of Rubisco condensates! You're in for some stunning #cryoET of the pyrenoid - in Chlamy, of course 😉 Congrats to all involved!

James Barrett@james-r-barrett.bsky.social · 6mo ago

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid

Please share! My group at @zmbp-tuebingen.bsky.social is offering a post-doctoral position (4 years). We look for a structural biologist with experience in Cryo-EM/Cryo-ET to investigate the mechanisms of host invasion by pathogenic fungi. Deadline February 28th! uni-tuebingen.de/universitaet...

LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery. Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure. 🔗 doi.org/10.64898/202... #CryoET

Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social Huge collaborative effort! So glad to see the community already using it to develop new resources & tools. Check it out here: shorturl.at/z4i4c #CryoEM #CryoET

We are thrilled to share our new pre-print on the molecular anatomy of nuclear proteasome in human sperm cells! #In-cellcryo-ET + #SPA + #LM! @piotrkolata.bsky.social @a-dsantos.bsky.social, @tomdendooven.bsky.social!! doi.org/10.64898/202...

IsoNet2 determines cellular structures at submolecular resolution without averaging https://www.biorxiv.org/content/10.64898/2025.12.09.693325v1