Arindam Ghosh, PhD
@arindam92.bsky.social
Research Group Leader, DKFZ NCT WERA #Biophysics #Imaging #Immunology #Receptors https://arindam-ghosh-lab.com/
(1/4) We are very excited to share our latest preprint from @sauer-lab.bsky.social: Mega Expansion Microscopy (Mega-ExM)! We combine iterative hydrogel expansion with whole-proteome NHS labeling and post-expansion immunostaining, reaching tunable expansion factors up to ~1500× in whole cells 🤯
bioRxiv Biophysics@biorxiv-biophys.bsky.social · 2mo agoStructural cell biology by mega-expansion microscopy https://www.biorxiv.org/content/10.64898/2026.08.05.743040v1
Got the last word in this great perspective on the state of fluorescent indicators in @nature.com by @dianakwon.bsky.social www.nature.com/articles/d41...
How genetically encoded sensors have lit up neuroscience
Tools that track specific molecules in neurons have enabled researchers to probe previously unexplored aspects of neurobiology — although important caveats remain.
nature.com
Happy to share that I will be starting my independent research career as a Research Group Leader at the German Cancer Research Center (DKFZ). Starting from January 2026, my group will be based at DKFZ National Center for Tumor Diseases (NCT WERA), Würzburg, Germany.
👏 Congratulation to the 🏆 winners of Student Award held by #PicoQuant during 30th anniversary of our annual workshop on Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Sciences. #WS30 We were proud to once again support the next generation of scientists. #PicoQuantStudentAward
🚨 New Preprint! Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons. ▶️ www.biorxiv.org/content/10.1...
Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
nature.com
Great news: I received ERC Advanced grant #ERCAdG @erc.europa.eu CaptuRel! It aims at intelligent nanomaterials undergoing bioinspired cycle of capture and photorelease of bioactive molecules for sensing and controlling (bio)chemical gradients. Thanks to my Team, @cnrs.fr and @unistra.fr!
De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...
🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...
New paper out! Would you like to fluorescently label the plasma membrane in live, fixed or permeabilized cells? I tested different lipid structures and labelling approaches to decide what makes a good membrane labelling probe for such imaging experiments! www.biorxiv.org/content/10.1...
Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure
Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membr...
biorxiv.org
Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩 We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨
JungmannLab@jungmannlab.bsky.social · last yr.Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution We are excited to present our latest work published in @natcomms.nature.com www.nature.com/articles/s41...
Collaborative work with the Shechtman Lab and @heilemannlab.bsky.social. Check it out here: www.biorxiv.org/content/10.1...
One-click image reconstruction in single-molecule localization microscopy via deep learning
Deep neural networks have led to significant advancements in microscopy image generation and analysis. In single-molecule localization based super-resolution microscopy, neural networks are capable of...
biorxiv.org
www.researchsquare.com/article/rs-6...
Open-source Sub-Nanometer Stabilization System for Super-resolution Fluorescence Microscopy
Recent advances in fluorescence nanoscopy have pushed resolution to the 1-10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments. Achieving this...
researchsquare.com
Our work on lattice light-sheet TDI-DNA-PAINT and anti-CD20 immunotherapy antibodies is now featured in The Scientist magazine www.the-scientist.com. Many thanks to Sneha Khedkar for writing this fantastic piece. www.the-scientist.com/new-microsco...
New Microscopy Technique Challenges Therapeutic Antibody Classification
A super-resolution microscopy technique offers an unparalleled glimpse into how monoclonal antibodies bind to their targets on cancer cells to induce cell death.
the-scientist.com
🔬Happy to share our new preprint, where brightness is used to identify single molecule emission in 2D/3D, with a single camera ! Part of the PhD work of @laurent-le.bsky.social and Surabhi, great collaboration with @emmanuelfort.bsky.social from @instlangevin.bsky.social @ismolab.bsky.social #SMLM
bioRxivpreprint@biorxivpreprint.bsky.social · 2y agoBrightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy https://www.biorxiv.org/content/10.1101/2025.03.02.639924v1
Congratulations to Rice Chemistry Prof. Anna-Karin Gustavsson for receiving the NSF CAREER. @gustavssonlab.bsky.social #NSFCAREER #RiceChemistry
Anna-Karin Gustavsson on LinkedIn: Rice’s Gustavsson receives NSF CAREER Award to investigate dynamics of…
Very honored and grateful to receive the NSF CAREER Award! Big thanks to the National Science Foundation (NSF) and to my fantastic research team and…
linkedin.com
Happy to share our newest paper in Ang. Chem. and big thanks to @nkaredla.bsky.social, @faldalf.bsky.social and @tao-chen.bsky.social 🙏 onlinelibrary.wiley.com/doi/10.1002/...
Leaflet‐specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers
Polymer-supported or tethered lipid bilayers serve as versatile platforms for mimicking plasma membrane structure and dynamics, yet the impact of polymer supports on lipid bilayers remains largely un...
onlinelibrary.wiley.com
Science, research & public health face unprecedented attacks in the U.S. We stand with our American colleagues & support the Stand Up for Science call. Join the global march tomorrow, incl. in Paris! 🧪✊ 📍 March 7, 13:30 – Place Jussieu 🔗 More info: standupforscience.fr #StandUpForScience
I'm thrilled to share that our review article, "Molecular Level Super-Resolution Fluorescence Imaging," has now been published online (www.annualreviews.org/content/jour...)! We provide a comprehensive overview of fluorescence super-res methods that push the limits towards molecular-scale imaging.
Molecular Level Super-Resolution Fluorescence Imaging | Annual Reviews
Over the last 30 years, fluorescence microscopy, renowned for its sensitivity and specificity, has undergone a revolution in resolving ever-smaller details. This advancement began with stimulated emis...
annualreviews.org
Online now! Fluorescent labeling strategies for molecular bioimaging. Marcel Streit, Made Budiarta, Marvin Jungblut, and Gerti Beliu. www.cell.com/biophysrepor...
Fluorescent labeling strategies for molecular bioimaging
Super-resolution microscopy (SRM) has transformed biological imaging by circumventing the diffraction limit of light and enabling the visualization of cellular structures and processes at the molecula...
cell.com
🆕 Our new functionalized docetaxel probe enables superior visualization of dense mitotic spindle structures during cell division by expansion microscopy! ▶️ pubs.acs.org/doi/10.1021/...
Functionalized Docetaxel Probes for Refined Visualization of Mitotic Spindles by Expansion Microscopy
Visualizing the ultrastructure of mitotic spindles, the macromolecular machines that segregate chromosomes during mitosis, by fluorescence imaging remains challenging. Here we introduce an azido- and ...
pubs.acs.org
Check it out, powerful new method for optical imaging of the live ECM, from @hhmijanelia.bsky.social Group Leader Kayvon Pedram #proudofalumni #glycotime www.nature.com/articles/s41...
Live imaging of the extracellular matrix with a glycan-binding fluorophore - Nature Methods
Rhobo6 is a cell-impermeable small-molecule fluorophore that displays reversible fluorogenic binding to glycans, making it a general, wash-free, and non-perturbative label for the extracellular matrix...
nature.com
Aaaand that’s a wrap at Asia Pacific Microscopy Congress 2025 in sunny Brisbane, attended by a big team from Single Molecule Science. Lots of exciting new 🔬 tools and translation in biomedical investigations. @ijayas.bsky.social @arindam92.bsky.social
Want to explore the world of time-resolved fluorescence #microscopy? Sign up for our online course and benefit from theoretical as well as practical sessions in an interactive format. Topics: data analysis, FCS, FRET, FLIM, and more. May 13-16, 2025. ➡️ www.microscopy-course.org
Thrilled and honored to present a plenary talk at the 13th Asia Pacific Microscopy Congress (APMC13) in Brisbane, Australia, on February 7, 2025, hosted by the Australian Microscopy and Microanalysis Society (AMMS).
New paper out💥 in Nano Letters @pubs.acs.org High throughput single particle profiler with simple confocal imaging. We used this new method to study protein-lipid interactions, drug membrane interactions and biophysics of small particles from human donors: pubs.acs.org/doi/10.1021/...
Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy
Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high t...
pubs.acs.org
🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.
If you have a passion for single-molecule super-resolution microscopy🔬, there are great news to share: the SMLMS conference series will continue this year and will be held in Bonn/Germany Aug 27th to 29th ✨- please save the date and join us, we are looking forward to meeting you there👇🏻
SMLMS 2025@smlm-symposium.bsky.social · 2y agoWelcome to our blue space! SMLMS 2025 is actively in the making! Soon we will be able to reveal our amazing speaker line-up here in Bonn! Stay tuned! While waiting, maybe already bookmark smlms.org! 👈 See you in August 2025 in Bonn!
How can you enhance the visibility of your next publication or increase your chances of getting your next research proposal accepted? How to get the illustration you need in just a few days? How to find an illustrator who is on the same wavelength with you?😉
